常用荧光染料的激发及发射波长

荧光素名称激发波长(nm)发射波长(nm)备注1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM(5-Carboxyfluorescein)4925185-HAT(HydroxyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamine)5785676045915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5466477-Hydroxy-4-methylcoumarin360449,4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow470550Acriflavin436520AcriflavinFeulgenSITSA355-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFPAlexaFluor350™346342442441AlexaFluor430™431540AlexaFluor488™495,492519,520AlexaFluor532™531,532553,554AlexaFluor546™556,557572,573AlexaFluor568™577,578603AlexaFluor594™590,594617,618AlexaFluor633™632650AlexaFluor647™647666AlexaFluor660™668698AlexaFluor680™679702AlizarinComplexon530-560,580580,624-645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345347425444AMCA-X353442AminoactinomycinD555655Aminocoumarin346350442445Aminomethylcoumarin(AMCA)345347425444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-Cy7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYellow7GLL450480Atabrine436490ATTO-TAG™CBQCA465560ATTO-TAG™FQ486591Auramine460550AurophosphineG450580Aurophosphine450-490515BAO9(Bisaminophenyloxadiazole)365395BCECF(highpH)492,503520,528BCECF(lowpH)482520BerberineSulphate430550BetaLactamase409447,520BFPblueshiftedGFP(Y66H)381,382,445,447,blueshiftedGFPBlueFluorescentProtein383448(Y66H)BlueFluorescentProteinBFP/GFPFRETBimane398490Bisbenzamide360461Bisbenzimide(Hoechst)360461bis-BTC=RatioDye,Zn2+455/405529/505RatioDye,Zn2+BlancophorFFG390470BlancophorSV370435BOBO™-1462481BOBO™-3570602Bodipy492/515490515Bodipy493/503533549Bodipy500/510509515Bodipy505/515502510Bodipy530/550528547Bodipy542/563543563Bodipy558/568558569Bodipy564/570564570Bodipy576/589579590Bodipy581/591584592Bodipy630/650-X625642Bodipy650/665-X647665Bodipy665/676605676BodipyFl504,505511,513BodipyFLATP505514BodipyFl-Ceramide505511BodipyR6GSE528547BodipyTMR542574BodipyTMR-Xconjugate544573BodipyTMR-X,SE544570BodipyTR589617BodipyTRATP591620BodipyTR-XSE588616BO-PRO™-1462481BO-PRO™-3544570BrilliantSulphoflavinFF430520BTC-RatioDyeCa2+464/401533/529RatioDyeCa2+BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+Calcein494517CalceinBlue373440CalciumCrimson™588,589611,615CalciumGreen501,506531CalciumGreen-1Ca2+Dye506531Ca2+DyeCalciumGreen-2Ca2+506/503536Ca2+CalciumGreen-5NCa2+506532Ca2+CalciumGreen-C18Ca2+509530Ca2+CalciumOrange549575576CalcofluorWhite385,395,405437,440,445Carboxy-X-rhodamine(5-ROX)576601CascadeBlue™377398399420423CascadeYellow399400550552Catecholamine410470CCF2(GeneBlazer)CFDA494520CFP-CyanFluorescentProtein430,433,436,(453)474,475,476,(501)CyanFluorescentProteinCFP/YFPFRETChlorophyll480650ChromomycinA436-460470ChromomycinA445575CL-NERF(RatioDye,pH)504/514540RatioDye,pHCMFDA494520CoelenterazineCa2+Dye,bioluminescence(429)465Ca2+Dye,bioluminescence,nativemoleculeCoelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescenceCoelenterazinef(437)473Ca2+Dye,bioluminescenceCoelenterazinefcp452Ca2+Dye,bioluminescenceCoelenterazineh(437)464Ca2+Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+Dye,bioluminescenceCoelenterazineip441Ca2+Dye,bioluminescenceCoelenterazinen(431)467Ca2+Dye,bioluminescenceCoelenterazineO460575CoumarinPhalloidin387470C-phycocyanineCPMMethylcoumarin384469MethylcoumarinCTC400-450602CTCFormazanCy2™489506Cy3.18554568Cy3.5™581598Cy3™514552554566570Cy5.18649666Cy5.5™675695Cy5™649666670Cy7™710,743767,805CyanGFP433(453)475(501)cyclicAMPFluorosensor(FiCRhR)500517CyQuantCellProliferationAssay480520CellProliferationAssayDabcyl453Dansyl340578DansylAmine337517DansylCadaverine335518DansylChloride372518DansylDHPE336517Dansylfluoride356noneDAPI359461Dapoxyl403580Dapoxyl2374574Dapoxyl3373574DCFDA504529DCFH(DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR(Dihydorhodamine123)505534Di-4-ANEPPS496705Di-8-ANEPPS(non-ratio)488498605713DiA(4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate(DCFH)505535DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5))644665DIDS341415Dihydorhodamine123(DHR)505535DiI(DiIC18(3))549,551565Dinitrophenol349DiO(DiOC18(3))484,487501,502DiR748780LipophilicTracerDiR(DiIC18(7))750779DM-NERF(highpH)497/510540RatioDye,pHDNP349Dopamine340490-520DsRed558583RedfluorescentproteinDTAF494520DY-630-NHS621660HemicyanelabelforproteinsandDNADY-635-NHS634664Hemicyanelabelforpr