eric-pcr

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,625014)ERIC,44bpERIC,ERIC2PCR,,ERIC;IRU;ERIC2PCR;;Q503A0517-6611(2007)18-05358-03CharacteristicandApplicationoftheEnterobacterialRepetitiveIntergenicConsensus2PCRLIYou2pingetal(CollegeofAnimalScienceandTechnology,SichuanAgriculturalUniversity,Yaan,Sichuan625014)AbstractEnterobacterialRepetitiveIntergenicConsensus(ERIC),akindofshortduplicationsequences,isfoundtoexistinthegenomeofprokaryoticor2ganism.Distributionandcopyofthesequenceinchromosomeisinterspecificspecificity.Accordingtothehighlyconservativecoresequence,44bp,reverseprimerisdesigned.ERIC2PCRcangenerateuniquefingerprintstoreflectthestructuralfeatureofbacterialstrains,andcanidentificationspecies.ERIC2PCRtechniquecanbeusedintheidentificationofbacterium,environmentalmonitoring,investigationintestinalfloraofhumanbeingsandanimals,whichisrapid,simpleandrepetitive.KeywordsERIC;IRU;ERIC2PCR;Characteristic;Application,,,,,PFGERFLPARDRARAPD2PCRDGGE2PCRERIC2PCR,,ERIC2PCRERICPCR,DNA,DNA,,1(ERIC)1990,Sharples[1]Escherichiacolitlsds3ERIC,[IRU(IntergenicRepetitiveUnit)],Hulton[2],ERIC(En2terobacterialRepetitiveIntergenicConsensus),[3]46,ERIC,,ERIC[4]2ERICERIC126bp,,,ERIC,,,Vibrocholerae,ERICsul5[2](1977-),,,,:3,,2007203223ERIC,3ERIC,3,;ERIC,DNA,,,;ERIC,mRNA;ERIC[5-7]3ERIC2PCR1999,Franklin[8]Wikstrom[9]RAPD,,,,,[10]ERIC2PCRPCR(RAPD)DNAERIC,44bpVersalovic[11]ERIC(ERIC152ATGTAAGCTCCTGGGGATTCAC23,ER2IC252AAGTAAGTGACTGGGGTGAGCG23),ERIC2PCRERIC,ERIC,DNA,,ERIC2PCRGillings[12],ERIC2PCRRAPD2PCR,,,RAPD2PCR,(22),PCR(RAPD2PCR40,ERIC2PCR52),,(503000bp),(),JournalofAnhuiAgri.Sci.2007,35(18):5358-5360©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[13];DNA,[14]4ERIC2PCR,ERIC2PCRERIC1997Gillings[12]DNA,ERIC,ERICGillingsERIC2PCRPCR(RAPD2PCR)[15]MG1655ERIC2PCR,ERIC2PCRERICDNA40,ERICERIC2PCR3kb,,ERICE.coliK212MG16551.1kb,,ERIC,ERIC,ERIC,ERICERIC,,PCR,,,ERIC,ERIC2PCRERIC,;ERIC,ERIC2PCR,(22),ERIC2PCRPCR5ERIC2PCRERIC2PCR,,,[16]5.1[17],ERIC2PCR[18219]62ERIC2PCR,,[20]ERIC2PCR5,TY7210,(ATCC1.1849),ERIC2PCR,ERIC2PCR,REP2PCR[21];89,ERIC2PCR[22];Rasschaert[23]5[(ERIC1R2ERIC2RREPIR2REP2IER2IC2RBOXAIR(GIG)5]PCR,ERIC1R2ERIC2R(GIG)5,3,1322;,3,5.21999DiGiovanni[24]ERIC2PCR,,RAPD2PCRARDRA,ERIC2PCR,[25]Gonzalez[26]ERIC2PCR,,;,,[27]DH5E226R,DNA,ERIC2PCR,DNA,;[28],ERIC2PCR,5.3ERIC2PCRKhan[29]ER2IC2PCR,[30]30DNAERIC2PCR,3027,,,[31]ERIC2PCRSouthern,,,,,ERIC2PCR,6GenomestoLife,,DNAERIC2PCR,,ERIC2PCRDNA,95353518ERIC2PCR©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[1]SHARPLESGJ,LLOYDRG.AnovelrepeatedDNAsequencelocatedintheintergenicregionsofbacterialchromosomes[J].NucleicAcidsResearch,1990,18(22):6503-6508.[2]HULTONCSJ,HIGGINSCF,SHARPPM.ERICsequences:anovelfamilyofrepetitiveelementsinthegenomesofEscherichiacoli,Salmonellatyphimuriumandotherenterobacteria[J].MolecularMicrobiology,1991,5(4):825-834.[3],.ERIC[J].,2002,25(4):354-357.[4]BACHELLIERS,CLEMENTJM,HOFNUNGM.ShortpalindromicrepetitiveDNAelementsinenterobacteria:asurvey[J].ResearchinMicrobiology,1999,150:627-639.[5]MERIONE,BECERRILB,VALLEF,etal.Deletionofarepetitiveextrgenicpalindromic(REP)sequencedownstreamformthestructuralgeneofEscherichiacoliglutamatedehydrogenaseaffectsthestabilityofitsmRNA[J].Gene,1986,50(2/3):305-309.[6]GILSONE,CLEMENTJM,PERRIND,etal.Palindromicunits:acaseofhighlyrepetitiveDNAsequenceinbacteria[J].TrendsinGenetics,1987(3):226-230.[7]GILSONE,PERRIND,HOFNUNGM.DNApolymeraseIandaproteincomplexbindspecificallytoE.colipalindromicunithighlyrepetitiveDNA:implicationsforbacterialchromosomeorganization[J].NucleicAcidsResearch,1990,18(13):3941-3952.[8]FRANKLINRB,TAYLORDR.CharacterizationofmicrobialcommunitiesusingrandomlyamplifiedpolymorphicDNA(RAPD)[J].JournalofMicrobiologicalMethods,1999,35(3):225-235.[9]WIKSTROMP,ANDERSSONAC,FORSMANM.Biomonitoringcomplexmicro2bialcommunitiesusingrandomamplifiedpolymorphicDNAandprincipalcom2ponentanalysis[J].FEMSMicrobiologyEcology,1999,28(2):131-139.[10],.[J].:,2000,7(2):48-51.[11]VERSALOVICJ,KOEUTHT,LUPSKIJR.DistributionofrepetitiveDNAse2quencesineubacteriaandapplicationtofingerprintingofbacterialgenomes[J].NucleicAcidsRes,1991,19(24):6823-6831.[12]GILLINGSM,HOLLEYM.RepetitiveelementPCRfingerprinting(rep2PCR)usingenterobacterialrepetitiveintergenicconsensus(ERIC)primersisnotnecessarilydirectedatERICelements[J].LettersinAppliedMicrobiology,1997,25(1):17-21.[13]DALLA2COSTALM,IRINOK,RODRIGUESJ,etal.Characterisationofdiar2rhoeagenicEscherichiacoliclonesbyribotypingandERIC2PCR[J].JournalofMedicalMicrobiology,1998,47(3):227-234.[14]HOUFK,ZUTTERLD,HOOFJV,etal.AssessmentofthegeneticdiversityamongarcobactersisolatedfrompoultryproductsbyusingtwoPCR2basedtyp2ingmethods[J].AppliedandEnvironmentalMicrobiology,2002,68(5):2172-2178.[15],,.MG1655ERIC2PCR[J].,2002,29(6):28-33.[16],.[J].,2003,43(2):264-270.[17],,.[J].,2005,32(2):5-9.[18],,.ERIC2PCR[J].,2003,19(7):879.[19],,.ERIC2PCR[J].,2003,25(2):195-197.[20]