分子生物学专家

Page 1 of 3 EXAMINATIONCONTENTGUIDELINEEXAMINATIONMODELTheMB(ASCP)andMB(ASCPi)certificationexaminationiscomposedof100examinationquestionsgivenina2hour30minutetimeframe.Allexaminationquestionsaremultiple-choicewithonebestanswer.Thecertificationexaminationisadministeredusingtheformatofcomputeradaptivetesting(CAT).WithCAT,whenapersonanswersaquestioncorrectly,thenexttestquestionhasaslightlyhigherlevelofdifficulty.Thedifficultylevelofthequestionspresentedtotheexamineecontinuestoincreaseuntilaquestionisansweredincorrectly.Thenaslightlyeasierquestionispresented.Inthisway,thetestistailoredtotheindividual’sabilitylevel.Eachquestioninthetestbankiscalibratedforlevelofdifficultyandisassignedacontentareathatmatcheswiththesubtestareaofthecontentoutlineforaparticularexamination.Theweight(value)giventoeachquestionisdeterminedbythelevelofdifficulty.Therefore,theexamineemustanswerenoughdifficultquestionstoachieveascoreabovethepasspointinordertosuccessfullypassthecertificationexamination.EXAMINATIONSUBTESTSTheMB(ASCP)andMB(ASCPi)certificationexaminationquestionsencompassfourdifferentsubtestswithintheareaofMolecularBiology:MolecularScience,MolecularTechniques,LaboratoryOperations,andApplicationsofMolecularTesting.Eachofthesesubtestscomprisesaspecificpercentageoftheoverall100-questioncertificationexamination.Thesubtestsfortheexaminationaredescribedinthefollowingtable:SUBTESTSDESCRIPTIONEXAMPERCENTAGESMolecularScience(MS)BasicstructureandfunctionasrelatedtoprinciplesandtheoryofMolecularBiology25-30%MolecularTechniques(MT)Basicprinciplesandselect,prepare,perform,optimize,troubleshoot,andevaluateresultsofMolecularTechniques20-30%LaboratoryOperations(LO)Contamination,QualityAssurance,Guidelines/Regulations,Personnel,Safety15-25%ApplicationsofMolecularTesting(AMT)Disease-specificapplicationsofMolecularTechniques25-30%ForamorespecificoverviewofthefoursubtestareasontheMB(ASCP)andMB(ASCPi)certificationexamination,pleaserefertotheCONTENTOUTLINEonpages2–3.TECHNOLOGISTINMOLECULARBIOLOGY,MB(ASCP)INTERNATIONALTECHNOLOGISTINMOLECULARBIOLOGY,MB(ASCPi)EXAMINATIONCONTENTGUIDELINE&OUTLINEJune2014©AmericanSocietyforClinicalPathologyPage 2 of 3 EXAMINATIONCONTENTOUTLINETECHNOLOGISTINMOLECULARBIOLOGYINTERNATIONALTECHNOLOGISTINMOLECULARBIOLOGYExaminationquestions,whicharerelatedtothesubtestareasoutlinedbelow,willbeboththeoreticalandprocedural.Theoreticalquestionsmeasureskillsnecessarytoapplyknowledge,calculateresults,andcorrelatepatientresultstodiseasestates.Proceduralquestionsmeasureskillsnecessarytoperformlaboratorytechniques,evaluatelaboratorydata,andfollowqualityassuranceprotocols.I.MOLECULARSCIENCE(25-30%)A.NucleicAcidChemistry1.Sugars2.Bases3.Chemicalstructure4.Associatedproteins5.MutationsB.BasicMolecularTheory1.Replication2.Transcription3.Exons,introns,andsplicing4.Translation5.Chromosomestructure6.Extrachromosomalstructure(e.g.,phage,plasmid,mitochondrial)7.ProteinstructureC.BiochemicalReagents1.Polymeraseenzymesa.DNAb.RNA2.Endoandexonucleaseenzymes3.Reversetranscriptase4.DNAligase5.AssaydevelopmentanddesignD.Genetics1.Human2.MicrobialII.MOLECULARTECHNIQUES(20-30%)A.SeparationandDetection1.Electrophoresisa.Gel(includingagaroseacrylamideandpulsedfield)b.Capillary2.Blottingandprobingprocedures(includingwashingandstringency)3.Probehybridization4.Nucleicacidpurification5.Probestructure(e.g.,TaqMan,FRET,simple,beacon,scorpion)B.NucleicAcidAmplification1.Polymerasechainreaction(PCR)2.PCRvariations(e.g.,real-time,nested,multiplex,arrays,reversetranscriptase)3.BranchedDNA(bDNA)technology4.Sequencebased(NASBA)5.Transcription-mediatedtechnology(TMA)6.Stranddisplacementamplification(SDA)7.Loop-mediatedisothermalamplification(LAMP)8.Other(e.g.,hybridcapture,ligasechainreaction,cleavase)C.DNASequenceAnalysis1.SangerSequencing(e.g.,chainterminators)2.Nextgenerationsequencing3.Automatedsequenceanalyzer4.Other(e.g.,pyrosequencing)D.OtherTechniques1.DenaturingHPLC2.Meltcurvesanalysis3.Nucleicacidlabeling4.in-situhybridization(ISH)5.Restrictionfragmentlengthpolymorphism(RFLP)6.Epigeneticmodification7.Arraytechnology(e.g.,bead,microarray)8.Multiplexligation-dependentprobeamplification(MLPA)9.Massspectrophotometry10.Multi-locussequencetyping(MLST)Page 3 of 3 III.LABORATORYOPERATIONS(15-25%)A.Contamination(e.g.,biological,amplifiedandnon-amplifiednucleicacid)1.Prevention2.Monitoringanddetection3.EliminationB.QualityAssurance1.Specimencollection,preparation,transportandstoragea.Evaluatequalityandquantityofspecimenb.Evaluatequalityandquantityofnucleicacid2.Reagentselection,preparation(includingcalculations),storage,anddisposal3.Assayselectionandvalidation4.Resultcalculation,interpretationandreporting5.Qualitycontrolandproficiencytestinga.Assaycontrolsb.Proficiencytesting6.EquipmentandInstrumentation–principles,calibration,maintenance,troubleshootingandvalidationC.GuidelinesandRegulations1.TestSystemCategories:Analytespecificreagent(ASR),researchuseonly(RUO),in-vitrodiagnostics(IVD)andlabdevelopedtests(LDT)2.RegulationsandStandards:CLIA,TheJointCommission,CAP,CMS,