E.-coli-常用菌株大全

E.coligenotypesContents[hide]1Nomenclature&Abbreviations2MethylationIssuesinE.coli3Commonlyusedstrainso3.1AG1o3.2AB1157o3.3B2155o3.4BL21o3.5BL21(AI)o3.6BL21(DE3)o3.7BL21(DE3)pLysSo3.8BNN93o3.9BNN97o3.10BW26434,CGSCStrain#7658o3.11C600o3.12C600hflA150(Y1073,BNN102)o3.13CSH50o3.14D1210o3.15DB3.1o3.16DH1o3.17DH5αo3.18DH5αTurbo(NEB)o3.19DH10B(Invitrogen)o3.20DH12S(Invitrogen)o3.21DM1(Invitrogen)o3.22E.cloni(r)5alpha(Lucigen)o3.23E.cloni(r)10G(Lucigen)o3.24E.cloni(r)10GF'(Lucigen)o3.25E.coliK12ER2738(NEB)o3.26ER2566(NEB)o3.27ER2267(NEB)o3.28HB101o3.29HMS174(DE3)o3.30High-Control(tm)BL21(DE3)(Lucigen)o3.31High-Control(tm)10G(Lucigen)o3.32IJ1126o3.33IJ1127o3.34JM83o3.35JM101o3.36JM103o3.37JM105o3.38JM106o3.39JM107o3.40JM108o3.41JM109o3.42JM109(DE3)o3.43JM110o3.44JM2.300o3.45LE392o3.46Mach1o3.47MC1061o3.48MC4100o3.49MG1655o3.50OmniMAX2o3.51OverExpress(tm)C41(DE3)(Lucigen)o3.52OverExpress(tm)C41(DE3)pLysS(Lucigen)o3.53OverExpress(tm)C43(DE3)(Lucigen)o3.54OverExpress(tm)C43(DE3)pLysS(Lucigen)o3.55Rosetta(DE3)pLysSo3.56Rosetta-gami(DE3)pLysSo3.57RR1o3.58RV308o3.59SOLR(Stratagene)o3.60SS320(Lucigen)o3.61STBL2(Invitrogen)o3.62STBL3(Invitrogen)o3.63STBL4o3.64SURE(Stratagene)o3.65SURE2(Stratagene)o3.66TG1(Lucigen)o3.67TOP10(Invitrogen)o3.68Top10F'(Invitrogen)o3.69W3110o3.70WM3064o3.71XL1-Blue(Stratagene)o3.72XL1-BlueMRF'(Stratagene)o3.73XL2-Blue(Stratagene)o3.74XL2-BlueMRF'(Stratagene)o3.75XL1-Red(Stratagene)o3.76XL10-Gold(Stratagene)o3.77XL10-GoldKanR(Stratagene)4Othergenotypeinformationsources5ReferencesNomenclature&AbbreviationsAlistedgenenamemeansthatgenecarriesalossoffunctionmutation,aΔprecedingagenenamemeansthegeneisdeleted.Ifageneisnotlisted,itisnotknowntobemutated.ProphagespresentinwtK-12strains(F,λ,e14,rac)arelistedonlyifabsent.E.coliBstrainsarenaturallylon-anddcm-.F-=DoesnotcarrytheFplasmidF+=CarriestheFplasmid.ThecellisabletomatewithF-throughconjugation.F'[]=CarriesanFplasmidthathashostchromosomalgenesonitfromapreviousrecombinationevent.ThiscellcanalsomatewithF-throughconjugation.ChromosomalgenescarriedintheFplasmidarelistedinbrackets.rB/K+/-=The(B/K)definesthestrainlineage.The+/-indicateswhetherthestrainhasorhasn'tgottherestrictionsystem.mB/K+/-=The(B/K)definesthestrainlineage.The+/-indicateswhetherthestrainhasorhasn'tgotthemodification(methylation)system.hsdS=Bothrestrictionandmethylationofcertainsequencesisdeletedfromthestrain.IfyoutransformDNAfromsuchastrainintoawildtypestrain,itwillbedegraded.hsdR=ForefficienttransformationofclonedunmethylatedDNAfromPCRamplificationsINV()=chromosomalinversionbetweenlocationsindicatedahpC=mutationtoalkylhydroperoxidereductaseconferringdisulfidereductaseactivityara-14=cannotmetabolizearabinosearaD=mutationinL-ribulose-phosphate4-epimeraseblocksarabinosemetabolismcycA=mutationinalaninetransporter;cannotusealanineasacarbonsourcedapD=mutationinsuccinyldiaminopimelateaminotransferaseleadstosuccinateor(lysine+methionine)requirementΔ()=chromosomaldeletionofgenesbetweenthelistedgenes(mayincludeunlistedgenes!)dam=adeninemethylationatGATCsequencesexist;highrecombinationefficiency;DNArepairturnedondcm=cytosinemethylationatsecondCofCCWGGsitesexist.dam&dcmarethedefaultpropertiesandalwayselided,whiledam-ordcm-shouldbedeclareexplicitlyDE3=LysogenthatencodesT7RNApolymerase.UsedtoinduceexpressioninT7-drivenexpressionsystemsdeoR=regulatorygenethatallowsconstitutiveexpressionofdeoxyribosesynthesisgenes;permitsuptakeoflargeplasmids.SeeHanahanD,USPatent4,851,348.***Thishasbeencalledintoquestion,astheDH10BgenomesequencerevealedthatitisdeoR+.SeeDurfee08,PMID18245285.dnaJ=oneofthechaparoninsinactivated;stabilizessomemutantproteinsdut1=dUTPaseactivityabolished,leadingtoincreaseddUTPconcentrations,allowinguracilinsteadofthymineincorporationinDNA.StableUincorporationrequiresunggenemutationaswell.endA1=ForcleanerpreparationsofDNAandbetterresultsindownstreamapplicationsduetotheeliminationofnon-specificdigestionbyEndonucleaseI(e14)=excisableprophagelikeelementcontainingmcrAgene;presentinK-12butmissinginmanyotherstrainsgalE=mutationsareassociatedwithhighcompetence,increasedresistancetophageP1infection,and2-deoxygalactoseresistance.galEmutationsblocktheproductionofUDP-galactose,resultingintruncationofLPSglycanstotheminimal,innercore.TheexceptionalcompetenceofDH10B/TOP10isthoughttobearesultofareducedinterferencefromLPSinthebindingand/oruptakeoftransformingDNA.galE15isapointmutationresultinginaSer123-Pheconversionneartheenzyme'sactivesite.SeevanDie,etal.PMID6373734,Hanahan,etal.PMID1943786,andEcoSalISBN1555811647.--Dcekiert16:56,23January2008(CST)galk=mutantscannotmetabolizegalactoseandareresistantto2-deoxygalactose.galK16isanIS2insertion~170bpdownstreamofthegalKstartcodon.SeeEcoSalISBN1555811647.--Dcekiert16:56,23January2008(CST)galU=mutantscannotmetabolizegalactosegor=mutationinglutathionereductase;enhancesdisulphidebondformationglnV=suppressionofamber(UAG)stopcodonsbyinsertionofglutamine;requiredforsomephagegrowthgyrA96=mutationinDNAgyrase;conveysnalidixicacidresistance