中药质量评价技术的国内外研究现状及分析

综述进展中药质量评价技术的国内外研究现状及分析陶燕蓉1,陈曦2[]:分析中药质量评价研究现状,探讨中药质量评价的方法发展趋势:查阅近年有关中药质量评价相关文献,通过分析归纳与综合进行评述:目前中药质量控制主流仍然是以化学成分为中心的质量控制,但由于其在中药质量控制中所显示出的局限性,多种替代方法应运而生,并均表现出一定的优势:对具多成分特点的中药,从生物活性特征出发建立适合中药质量控制的新方法不仅必要,而且完全可行[]中药质量;评价技术;生物活性;Caco-2单层细胞模型;指纹图谱[]R2-031[]A[]1674-926X(2011)01-0059-04CurrentstatusofTraditionalChineseMedicine(TCM)qualityevaluationmethodsathomeandabroad/TAOYanrong1,CHENXi2//(1.DongZhMienHospitalofPekingTCMUniversity,Beijing100101,China;2.InstituteofMedicinalPlantDevelopment,ChineseAcademyofMedicalScienceandPekingUnionMedicalCollege,Beijing100193,China)[Abstract]Objective:ToanalyzethecurrentstatusanddiscussthedevelopmenttrendofTraditionalChineseMedicine(TCM)qualityevaluationmethods.Method:RelatedLiteratureswereresearchedinrecentyearsandreviewedbyanalysis,inductionandsynthesis.Result:ThemainstreamofTCMqualitycontrolbasedonchemicalconstituentatpresen.tBecauseofitslimitation,othersuperiorsubstitutemethodshadappeared.Conclusion:BecauseofthecomplicatedcompositionofTCM,itisnecessaryandfeasibletoestablishsomenewmethodswhicharesuitableforTCMqualitycontrolbasedonbiologicalactivitycharacters.Thesemethodswillhavebroadprospects.[Keywords]TCMquality;evaluationmethod;biologicalactivity;Caco-2cellmonolayermodel;fingerprint[]()(200909502-021)[]1.,,1001012.,,100193[](1965),,Te:l(010)84013153[](1973),,,,Te:l(010)62829207Emai:[e-mail][]20101201,,,,,,,1()(),,,,,,,,,,[1],2005()572,60%,20%,5%,[2],59中药与临床PharmacyandClinicsofChineseMateriaMedica2011;2(2),();[3],,;[4],,GAP;[5],,;[6],(,,,,,)2(),,[7][8],,,50,[9]2005()[10],()[11]()[12]()[13,14]()[8],,,:2.1[15,16]DNA,[17~22][23,24](-)[25~30],,,,[31]2.2[32,33],-,,,,2.3[34~37],,,,,,,,,,,,,,33.1Caco-2()[38~40],,Caco-2,,HPLC-MS,,60中药与临床PharmacyandClinicsofChineseMateriaMedica2011;2(2),3.2[41]HPLC,HPLC,,,,HPLC,,()4,,()(),,,,,:(),,,()?,?,,,?()?,(,),-Caco-2()()(),(),(),,(),(),(),??,?[][1],,,.[J].,2007,32(14):1377.[2].[J].:,2006,8(3):8.[3],,.[J].,2000,22(6),395.[4].GAP[J].,2001,3(10):17.[5],,,.[J].,1999,44(6):588.[6],.[J].:,2001,3(4):42.[7],,,.[J].,2006,29(6):55.[8].[J].,2001,17(1):1.[9],,,.[J].:,2007,9(6):36.[10].()[S].:,2005,57.[11],,.()[J].,2002,25(6):409.[12],,,.[J].,2001,15(3):56.[13],.[J].,1997,28(1):18.[14].[M].:,2006:179.61中药与临床PharmacyandClinicsofChineseMateriaMedica2011;2(2)[15]BeigiF,YangQ,LundahlP.Immobilizedliposomechromatographicanalysisofdrugpartitioningintolipidbilayers[J].JChromatogrA,1995,704(2):315.[16],,,.[J].,2001,29(10):1135.[17],,,.[J].,2002,30(2):231.[18]MaoXQ,LongL,LuoQZ,etal.ScreeningandanalysisofpermeablecompoundsinPtadixAngelicaSinensiswithimmobilizedliposomechromatography[J].JChromatogrB,2002,779:331.[19],,,.[J].,2006,11(7):776.[20]ShengLH,LiSL,KongL,etal.SeparationofcompoundsinteractingwithliposomemembraneincombinedprescriptionoftraditionalChinesemedicineswithimmobilizedliposomechromatography[J].JPharmBiomedAna,l2005,38(2):216.[21],,,.[J].,2004,32(12):1595.[22],,.[J].,2005,33(1):13.[23]WangH,ZouH,NiJ,etal.ComparativeseparationofbiologicallyactivecomponentsinRhizomachuanxiongbyaffinitychromatographywith1-acidglycoproteinandhumanserumalbuminasstationaryphases[J].Chromatographia,2000,52(7):459.[24]WangH,ZouH,NiJ,eta.lFractionationandanalysisofArtemisiacapillariesThunb.Byaffinitychromatographywithhumanserumalbuminasstationaryphase[J].JChromatogrA,2000,870:501.[25],.[J].:,2002,4(5):44.[26],,,.[J].,2002,30(2):195.[27]LundqvistA,LundahlP.Biomembraneaffinitycentrifugalanalysesofsoluteinteractionswithmembraneproteins[J].ChromatogrA,1999,852(1):93.[28]HaneskogL,ZengCM,LundqvistA,eta.lBiomembraneaffinitychromatographicanalysisofinhibitorbindingtothehumanredcellnucleosidetransporterinimmobilizedcells,vesiclesandproteoliposomes[J].BiochimBiophysActa,1998,1371(1):1.[29]HaneskogL,LundqvistA,LundahlP.Biomembraneaffinitychromatographicanalysisofnitrobenzyhhioinosinebindingtothereconstitutedhumanredcellnucleosidetransporter[J].JMolRecognit,1998,11(1-6):58.[30],,.[J].,1999,44(6):632.[31],,,.[J].,2007,5(1):1.[32],,,.[J].,2006,41(10):956.[33],,.TLC[J].,2005,36(1):132.[34],.[J].,2004:74.[35],,,.[J].,2007,32(14):1377.[36],,.:[J].,2002,27(5):393.[37],,.[J].,2004,35(11):1230.[38]ArturssonP,Correlationbetweenoraldrugabsorptioninhumanandapparentdrugpermeabilitycoefficientsinhumanintestinalepithelia(Caco-2)cells[J].BiochemBiophysResCommun,1991,175:880.[39],,.Caco-2[J].,2007,5(1):59.[40],,,.Caco-2[J].,2006,4(4):299.[41],,.-[A]..[M].:,2006,483.(:)62中药与临床PharmacyandClinicsofChineseMateriaMedica2011;2(2)