高效液相色谱仪性能的检验

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(197198)(,730000):O657.32:C:100623757(2009)0320197202.,,.(InstallationQualificationTest)(OperationalQualificationTest)(PerformanceQualificationTest).,2-3.,.,,.,.,,.,:[1].1,.:,AB,.,0.005in.PEEK,500psi.A,B0.1%/(V/V).UV,280nm,011-0.8AU.1.1,LC,.,(A,B,C,D).A,B0.1%,A-B,A-C,A-D.:2mL/min,100%A,4min,10%B,100%B,4min,..,%B.%B=-0%100%-0%x100(1)(QC)%B.,.,,.,,.1.2.1mL/min,15min0%100%B,100%B10min.1,,0%100%.QC,.:2009-06-29;:2009-09-01.:(1939-),,,,:.E-mail:[e-mail].©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(50%B).tG,,.,50%B10.2min,15min,VD=(10.2min-15min/2)x1mL/min=2.7mL.2C8C18.,//,1--4-..2.13(,20L60L).6,1--4-.RSD1%,,.2.21/2,2.1.100L,6RSD2%.3LC.1mL/min,10mL,10min2%(12s).4..(0.1mL/min),3000psi,.10,15%.,.,,.:[1]SnyderLR,DolanJW.High-performancegradientelution[M].Hoboken,NewJersey:JohnWiley&Sons,Inc,2007:145-151.891