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26820078FISHERIESSCIENCEVol.26No.8Aug.2007EPA1,2,1(1.,210093;2.,276005):EPA;;:Q914.82:C:100321111(2007)0820475203:2006-09-28;:2006-10-30.:(1970-),,,,:;E-mail:linyi_shi@163.com.:(1934-),,,,:;E-mail:liuzl@nju.edu.cn.,,(EPA,205n-3)EPA(),[1]EPA,,,EPA,[2]EPA,(Por2phyridiumcruentum)[3](Phaeodactylumtricornu2tum[425](Isochrysisgalbana)[6](Nannochloropsisoculata)(N.sp.)(Monodussubterraneus)[728]EPAEPA,,,EPA1()(photobioreactor),[9]:(1);(2);(3);(4)[10],0.45g/L,5cm,60%[11],:,[12],:(1);(2)CO2;(3),,(),(),,,CO2,,[13],2EPA,,Sukenik[14],816,EPA(3.8%);,,EPA1/2ChiniZit2telli[7]EPA,,,EPAEPA,[15]Richmond[16]PUFA,,,,[7,17]3,,,,,,,,,[18],,,,,[19]Hu[20](),(Sprulinaplatensis),[21],1.317cm,10cm,EPA,66.7278.2mg/m2,232.1515.7mg/m2,,,,4,CO2,,,[5][22],,,Asterio[23],0.01m/s,,0.02%0.04%(CMC),0.04m/s,5,EPA,[24]Garca[25]UTEX-640,,(C/N)C/N,[26](Chlo2rellasorokiniana),C/N20,[27]C/N,C/N6,,NaCl,Anna[28]NaCl41.8g/L(0.7mol/L),EPA,0.450.8mol/L[29]Cohen[30]0.252mol/LNaCl,NaClEPA,0.25mol/L,NaCl1mol/L,,430,EPA;2025,EPA[31]7,,20%30%,,,[32],,(FeCl3Al2(SO4)3),,,,[33],,-,,[34],,[35],Sukenik[36],EPA,EPA,8EPA,,,,,:[1]TapieroH,NguyenGB,CouvreurP,etal.Polyunsatu2ratedfattyacids(PUFA)andeicosanoidsinhumanhealthandpathologies[J].BiomedPharmacother,2002(56):2152222.[2]BarclayWR,MeagerKM,AbrilJR.Heterotrophicproductionoflongchainomega-3fattyacidsutilizingalgaeandalgae-likemicroorganisms[J].JApplPhy2col,1994(6):1232129.[3]CohenZ.ProductionofeicosapentaenoicandarachidonicacidbytheredalgaPorphyridiumpurpureum[G]//Ap2plewhite,T.H.(Ed.),ProceedingsofWorldConfer2enceonBiotechnologyfortheFatsandOilsIndustry.A2mericanOilChemists’sSociety,1988:2582287.[4]ChrismadhaT,BorowitzkaMA.Effectofcelldensityandirradianceongrowth,proximatecompositionandei2cosapentaenoicacidproductionofPhaeodactylumtricorn2utumgrowninatubularphotobioreactor[J].JApplPhy2col,1994(6):67274.[5]MolinaGrimaE,SanchezPerezJA.EffectofdilutionrateoneicosapentaenoicacidproductivityofPhaeodacty2lumtricornutumUTEX640inoutdoorchemostatculture[J].BiotechnolLett,1994(16):103521040.[6]MolinaGrimaE,GarcA,CamachoF,etal.OutdoorchemostatcultureofPhaeodactylumtricornutumUTEX67426640inatubularphotobioreactorfortheproductionofei2cosapentaenoicacid[J].BiotechnolApplBiochem,1994(20):2792290.[7]ChiniZittelliG,LavistaF.Productionofeicosapentae2noicacidbyNannochloropsissp.culturesinoutdoortu2bularphotobioreactors[J].JofBiotechnol,1999(70):2992312.[8]HuQ,HuZ,CohenZ,etal.Enhancementofeicosap2entaenoicacid(EPA)andS-linolenicacid(GLA)productionbymanipulatingalgaldensityofoutdoorcul2turesofMonodussubterraneus(Eustigmatophyta)andSpirulinaplatensis(Cyanobacteria)[J].JPhycol,1997(32):81286.[9]EiichiO,JoelLC.Designparametersofsolarconcentra2tingsystemsforCO2-mitigatingalgalphotobioreactors[J].Energy,2004(29):165121657.[10]SpektorovaL,CreswellRL,VaughanD.Closedtubu2larcultivators:aninnovativesystemforcommercialcul2tureofmicroalgae[M].WorldAquaculture,1997:39243.[11]RichmondA,VonshakA,AradSM.Algaebiomass[G]//ShelefG,SoederCJ,editors.NewYork:Elsevier;1980:65273.[12]MolinaGrimaE.Photobioreactors:lightregime,masstransfer,andscaleup[J].JournalofBiotechnology,1999(70):2312247.[13]WeissmanJC,GoebelRP,BenemannJR.Photobio2reactordesign:mixing,carbonutilization,andoxygenaccumulation[J].BiotechnolBioeng,1988(31):3362344.[14]SukenikA,ZmoraO,CarmeliY.Biochemicalqualityofmarineunicellularalgaewithspecialemphasisonlip2idcomposition..Nannochloropsissp[J].Aquacul2ture,1993(117):3132326.[15],.EPA[J].,2003(1):5210.[16]RichmondAE.Microalgaculture[J].CritRevBio2technol,1986(4):3682438.[17]RichmondA,ChengWZ.OptimizationofaflatplateglassreactorformassproductionofNannochloropsissp.Outdoors[J].JofBiotechnol,2001(85):2592269.[18]JanssenM,SlendersP,TramperJ,etal.PhotosyntheticefficiencyofDunaliellatertiolectaundershortlight/darkcycles[J].EnzymeMicrobiolTechnol,2001(29):2982305.[19]LawsEA.NutrientandlightlimitedgrowthofThalassi2osirafluiatilisincontinuousculturewithimplicationsforphytoplanktongrowthintheocean[J].LimnolOcean2ogr,1980(25):4552473.[20]HuQ,GutermanH,RichmondA.Aflatinclinedmod2ularphotobioreactorforoutdoormasscultivationofpho2toautotrophs[J].BiotechnolBioeng,1996(51):51260.[21]ZouN,RichmondA.Effectoflight-pathlengthinoutdoorflatplatereactorsonoutputrateofcellmassandofEPAinNannochloropsissp.[J].JournalofBiotech2nology,1999(70):3512356.[22],.[J].,1997,13(1):91297.[23]AsterioSM.Shearstresstoleranceandbiochemicalchar2acterizationofPhaeodactylumtricornutuminquasisteady-statecontinuouscultureinoutdoorphotobiore2actors[J].JBiochemicalEngineering,2003(16):2872297.[24]MayoAW,NoikeT.EffectofglucoseloadingonthegrowthbehaviorofChlorellavulgarisandheterotrotrophicbacteriainmixedculture[J].JWatRes,1994(28):100121008.[25]MC.GarcaC.MixotrophicgrowthofthemicroalgaPhaeodactylumtricornutuminfluenceofdifferentnitrogenandorganiccarbonsourcesonproductivityandbiomasscomposition[J].ProcessBiochemistry,2005(40):2972305.[26]RatledgeC.Biotechnologyofoilsandfats[G]//Ra2tledgeC,WilkinsonSG,editors.Microbiallipids,Vol.2.London:AcademicPress,1989:5672668.[27]ChenF,JohnsMR.Astrategy
本文标题:EPA的海洋微藻的规模化生产
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