从动物组织中提取线粒体的方法

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1.,210095;2.,510640;3.,510642),,ATP,,,,,,,,,,,,,,,,,,1,,,(),,,,1,2,,,,,2(1),,15min(2)ABC,3(1)8.08.5g,(2)50mlA,(3)A,,B100ml(4)(DY892I)(520s)(5)B140ml,50ml(6)600g10min(7)4,(8)10000g15min,,(9)B,1(10)10000g15min,,C(11)8000g10min,(12)3mlC(13)6,,,4(1)pH7.4A:Sucrose34.2,ED2TA2.92,Tris2HCl15.76,KCl3.4296,1000ml(2)pH7.4B:Sucrose34.2,EDTA2.92,Tris2HCl15.76,KCl3.4296,BSA5,1000ml(3)pH7.4C:41.906,Sucrose23.94,Tris2HCl3.152,KH2PO40.6845,EDTA22Na0.00584,1000ml5:JanusGreenB,,,,,,,,,5(1)10g/,1,3;,1g,750mg/,10(2)()0.06%,3(3),1,7(4),,,3,6,6,,,,,16ChineseJournalofVeterinaryMedicine2006(42)2©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1),,,(ATP,,)DNA,,,,(2),,,,,,2,,(3),,,(4),,,,,,(5),,,,,,,,(6),,,,(7)1(08)37,(-18),(25)12h,,,,,()1,2,3,4(1.,130118;2.,130051;3.,750105;4.,130061),,,,11:95088,5,,,,;10,,,,239,,104/min,,50/min;;,,;,;,,;,,:,,,,,,5,3,,,,,,199752919,50mg,3012,60mg,20mg,20,,,,,,,262006(42)2ChineseJournalofVeterinaryMedicine