桃花多糖的提取及其抗氧化活性研究

,,3(,041004):,SavageFentonBcauchampNBT,1mg/mL,5417%9417%,C,:;;;PrunuspersicaBatsch[1],,,,,,,,,,,20,,,,,,;,[2,3]1(),(),(),(),,,60,2211:30g,3,3h,,,380%,2h,,,,,212:120903h,,,4000r/min20min,,395%,24h,,,,[4,5]213:,Sevage,412(51),20min,,4000r/min,233,30%H2O25020min,3,95%,,60,,214:651ChineseTraditionalandHerbalDrugs4020093:2009205215:(30470296):(1985),E2mail:gczsxll@1631com3E2mail:guiqiny@dns1sxnu1edu1cn,,MolishFenling2[6,7]Molish,;,;2,;,21521511:[810]H2O2Fe2+Fenton,OH,OH,510nm,,Smironff,6mmol/LFeSO42mL(C)2mL,6mmol/LH2O22mL,,10min,6mmol/L2mL,,30min510nm(Ai),(Aj),(A0),,1=[1-(Ai-Aj)/A0]100%1CFig11ComparisonofeffectsbetweenpeachblossompolysaccharideextractsandvitaminCsolutiononhydroxylfreeradicalscavengingC1mg/mL,C5417%5116%,C,(P0105)21512:BcauchampNBT[11],110-4mol/L015mL0101mol/L1mL310-6mol/L015mL,015mL(C),pH7185mL,20W,,20min,,,560nm,[R=1/(A-A)100%],22CFig12ComparisonofeffectsbetweenpeachblossompolysaccharideextractsandvitaminCsolutiononultraoxygenanionscavnging,C,80%1mg/mlC9413%9012%,C,(P0105)3,,C,,,,,,,,,751ChineseTraditionalandHerbalDrugs402009:[1]1[M]1:,19831[2]1[J]1,1994,25(1),402441[3],1[J]1,2000,11(S),902981[4]FurutaH,TaroT,JyunkoT,etal1Extractionofwater2solublesoybeanpolysaccharidesunderacidicconditions[J]1BiosciBiotechBiochem,199812300223051[5],,,1[J]1,2007(2):342361[6]1[M]1:,19941[7]1[M]1:120031[8],,1[J]1,2007128422851[9],,1[J]1:,2007:572601[10],,,1[J]1,2006:1821[11],1[J]1,2008:18511,2,13,1,3,2(11302,100039;21,100037;31,110016):HPLCKromasilC18(250mm416mm,5m);2,;110mL/min;30;208nm9RSD3%,:;;PotentillaanserinaL1,,,,,,[17],SFDA,HPLC,1Agilent1100()()302,98%,10,302P1anserinaL1,12211:KromasilC18(250mm4161Table1SamplesourcesofRadixPotentillaeAnserlnae16273849510mm,5m);(A)2(B),:030min(20%B40%B),3040min(40%B55%B),4060min(55%B80%B);110mL/min;30;208nm212:,320200g/mL,0145m,213:210g,,70%50mL,12h,110h,70%,30%,D2101(15g),(150mL),70%(150mL),851ChineseTraditionalandHerbalDrugs4020093:2009209216:(2004BA901A25):(1981),,,Tel:[phone]E2mail:houluxing810620@1631com3Tel:[phone]E2mail:cgm1004@vip1sina1com