玉米芯酶解发酵制备木糖醇的研究

FOODSCIENCEANDTECHNOLOGY20103510XylitolpreparedfromcorncobwithenzymeandfermentationYUXian-chun1,2,LIXiang-su3(1.BasisDepartment,YueyangVocational&TechnicalCollege,Yueyang414000;2.CollegeofMaterialandEngineering,CentralSouthUniversityofForestryandTechnology,Changsha410001;3.AcademicAdministration,UniversityofSouthChina,Hengyang421002)Abstract:XylitolwasmadefromcorncobwithCandidatropicalis.Theeffectsofthetimeofculturetemperature,pHvalue,inoculationsizeandtheculturevolumeinflaskonxylitolyieldwerediscussed.Theprocesswasoptimizedandanalyzedwithresponsesurfacemethodology,andquadraticregressionmodelwasfounded.Theresultshowedthatthexylitolyieldwas76.35%asculturetemperaturewas32.4℃,pHvaluewas5.7,theculturevolumeinflaskwas68.7%andinoculationsizewas8.7%(v/v).Keywords:xylitol;corncob;enzymeandfermentation;RSM1,2，3(1.4140002.4100013.421002)：。、pH、4。。32.4℃、pH5.7、68.7%8.7%(v/v)76.35%。：：TQ923：A：1005-9989(2010)10-0039-04：2010-02-06：(08D124)。：(1969—)。、、。。、、。。·39·FOODSCIENCEANDTECHNOLOGY20103510。11.1≥。、、、℃。、、、、、、℃。、、℃。1.2～∶℃℃。℃。、、、。℃。。×22.1、、。。、、。℃℃。。℃。。℃、、。。·40·FOODSCIENCEANDTECHNOLOGY20103510℃、、。。。℃、、。。。2.2、、。。“”“”“”、。、。、。、。℃、、、。。1-101/℃A273033pHB567/%C81216/%D5060702FPProb＞F2887.1714206.2321.61＜0.0001A305.811305.8132.05＜0.0001pHB76.15176.157.980.0128C77.58177.588.130.0121D432.401432.4045.31＜0.0001143.14159.54126.211012.623.730.079716.9353.39R2＝0.9528·41·FOODSCIENCEANDTECHNOLOGY20103510。3。、、。、。℃、、、。：[1],,.[J].,2005,(4):547-551[2],,,.[J].,2008,(6):113-114[3],.[J].,2009,(3):63-64[4].[J].,2007,(6):8-11[5],,.[J].,2008,(26):50-52[6],.[J].,2006,6(6):88-89/%7469.2564.559.755516.0014.0012.0010.008.006.505.006.005.507.00B:pHD:5Y=f(A,C)Y=f(A,D)Y=f(B,D)7570.7566.562.255870.0065.0060.0055.0030.0050.0027.0028.5031.5033.00A:C:/%757065605516.0014.0012.0010.008.0027.0028.5030.0031.5033.00A:D:/%T2010'(CFE2010)11CFE2010T。。CFE2005CFE。、、、、CFE。2009CFECFE、。CFE、、T、、2010。、、。、、、。--T·42·