模式生物斑马鱼在中药研究中的应用-陈磊

434ActaPharmaceuticaSinica2012,47(4):434−4391,2*,1(1.,510006;2.,510515):,,:;;:R284:A:0513-4870(2012)04-0434-06ApplicationofzebrafishmodelorganismintheresearchofChinesemateriamedicaCHENLei1,LIUYi2*,LIANGSheng-wang1(1.SchoolofChinesePharmacy,GuangdongPharmaceuticalUniversity,Guangzhou510006,China;2.CollegeofTraditionalChineseMedicine,SouthernMedicalUniversity,Guangzhou510515,China)Abstract:ZebrafishhasbecomeanimportantmodelorganisminmanyfieldsofbiomedicalstudiesandbeenincreasinglyusedinChinesemateriamedicastudiesinrecentyears.ThisarticlesummarizedtheachievementsandprospectforzebrafishasapharmacologicalandtoxicologicaltoolinthestudyanddevelopmentofChinesemateriamedica.Keywords:zebrafish;modelorganism;Chinesemateriamedica,[1],2070,,[2],()():2011-10-27;:2011-12-30.:(81050019);(LYM10095).*Tel:[phone],Fax:[phone],E-mail:[e-mail],,,,,,1,;,,,,:4352070,[3],,IC50,[4]:,,24h,48h[5],,[6]3(13,1)[7],,24h,,3,Bliss(LC50),LC505.02×10−3113.21.18×103μg·mL−1,3LD50,(4,1)[8]:1μmol·L−1,234μmol·L−1,,,EC501.78μmol·L−1,[9]95%,,,,,,[10,11](5,1),60h72h,SODMDA[12]SODNa+-K+-ATPase,(6,1)LC5016.90mg·L−1,,SODNa+-K+-ATPase;SOD,Na+-K+-ATPase,[13](7,1),EC50(96hpf)51.17mg·L−1,,,,2,,,,,;,,,;87%,,,,[14],:[15][15][16][17][18][19][20][21],,——,[4]2.1,436ActaPharmaceuticaSinica2012,47(4):434−439,,,,,[22],Liang[23],(6-O-p-),;,Hong[24](95%,11),(sub-intestinalvessels),He[25],cangoronine(148,1),3:,1.2μmol·L−1Ang-2/Tie2mRNA,(50%)He[26,27](52%),(3910,1),20μmol·L−14872hpf,Ang-2/Tie2mRNAHe[28]1117:437,Liu[29][TG(fli1:EGFP)y1/+(AB)],250μg·mL−1;,,;,,(11,1),50μg·mL−1(P0.001)(Curcumawenyujin.)(),Zhong[30](12,1),,10μmol·L−1,50μmol·L−1(72h)5μmol·L−1SU5416,,Alexa[31](13,1)Chan[32]D(14,1),,[33,34],2550100mg·L−1,IC5029.6mg·L−1;,(inter-segmentalvessel,IV)(22.67±2.60),17.3%;IV(17.2±5.71),37%;,(P0.050.01)2.2,Richetti[35](1516,1)200μmol·L−1(18cm×9cm×7cm,L×W×H),1h(50mg·kg−1),(Tween-20,),,()[36],120h,Na+-K+-ATP,,Na+-K+-ATP,,,,[20]Pyun[37](17,1),,31981GeorgeStreisinger[38]Nature,20,,,[39],,,3050μm,,,,;,,,,,,,[4];,[40,41]438ActaPharmaceuticaSinica2012,47(4):434−439,,:,;,,;,,();,,,,——,,References[1]ZhaoXY,LiangSF,YaoSH,etal.IdentificationandpreliminaryfunctionstudyofXenopuslaevisDRR1gene[J].BiochemBiophysResCommun,2007,361:74−78.[2]LiuCS,MuY,DuJL.Applicationofthezebrafishintheresearchoflifesciences[J].ChinBullLifeSci(),2007,19:382−386.[3]WangJJ,XuC,TuY,etal.Experimentalresearchandapplicationofzebrafishandembryosintoxicology[J].AsianJEcotoxicol(),2007,2:123−135.[4]LiYY,HuangWJ,HuangC.Zebrafishindrugdiscovery[J].ChinBullLifeSci(),2011,23:273−379.[5]WesterfieldM.TheZebrafishBook[M].Eugene:UniversityofOregonPress,1995:16−21.[6]EnsenbachU,NagelR.Toxicityofcomplexchemicalmixtures:acuteandlongtermeffectsondifferentlifestageszebrafish(Brachydaniorerio)ecotoxicologyandenvironmental[J].EcotoxicolEnvironSafety,1995,30:151−157.[7]ShuB,WeiY,ZhangLY,etal.Acutetoxicityoftriptolide,matrineandemodinonzebrafish[J].JYunnanCollTraditChinMed(),2010,33:35−38.[8]WangSF,LiuKC,WangXM,etal.Preliminarystudyoncardiotoxicityofcelastroltozebrafishembryo[J].ChinPharmacolBull(),2009,25:634−636.[9]ZhangXM,ChenLH,ZhengRK,etal.HistopathologicalchangesofheartandbraininzebrafishexposuretoEupatoriumodoratumextract[J].JGuangdongOceanUniv(),2010,30:27−32.[10]HuangHL,LiuHG,MengY,etal.TheeffectofnitidinechlorideonSODactivityandMDAcontentofzebrafishembryo[J].JToxicol(),2011,25:243−246.[11]HuangHL,LiuHG,MengY,etal.Effectofnitidinechlorideontheheartdevelopmentofzebrafishembryos[J].GuangxiMedJ(),2011,33:546−548.[12]WangJN,HuangJ,HuangRB,etal.EffectsofcolchicineontheactivitiesofSODandNa+-K+-ATPaseinBrachyclaniorerio(Zebrafish)[J].ChinJNewDrugs(),2010,19:1247−1252.[13]TianLL,DongJY,HuangCJ.Effectofcurcumolonembryo-larvalstagesofzebrafish[J].JWenzhouMedColl(),2010,40:557−561.[14]LieschkeGJ,CurriePD.Animalmodelsofhumandisease:zebrafishswimintoview[J].NatRevGenet,2007,8:353−367.[15]LangenauDM,TraverD,FerrandoAA,etal.Myc-inducedTcellleukemiaintransgeniczebrafish[J].Science,2003,299:887−890.[16]LangheinrichU.Zebrafish:anewmodelonthepharmaceuticalcatwalk[J].Bioessays,2003,25:904−912.[17]CatherineTN,QingL,WangYB,etal.Zebrafishasamodelforcardiovasculardevelopmentanddisease[J].DrugDiscovToday,2008,5:135−141.[18]SullivanC,KimCH.Zebrafishasamodelforinfectiousdiseaseandimmunefunction[J].FishShellfishImmun,2008,8:341−350.[19]MilanDJ,MacRaeCA.Zebrafishgeneticmodelsforarrhythmia[J].ProgBiophysMolBiol,2008,98:301−308.[20]TonC,ParngC.Theuseofzebrafishforassessingototoxicandotoprotectiveagents[J].HearRes,2005,208:79−88.[21]LevinED,LmipuangthipJ,RachakondaT,etal.Timingofnicotineeffectsonlearninginzebrafish[J].Psychopharma-cology(Berl),2006,184:547−552.[22]RaghunathM,SyWongY,FarooqM,etal.Pharmacologicallyinducedangiogenesisintransgeniczebrafish[J].BiochemBiophysResCommun,2009,378:766−771.[23]LiangZT,HeMF,FongWF.Acomparable,chemicalandpharmacologicalanalysisofthetraditionalChinesemedicinalherbsOldenlandiadiffusaandO.corymbosaandanewvaluationoftheirbiologicalpotential[J].Phytomedicine,2008,15:259−267.:439[24]HongSJ,WanJB,Z