基因芯片技术在疟疾研究中的应用

2080,;;;;TheapplicationofgenechiptechnologyinmalariaresearchZHANGGuo2qing,TANGLin2hua.Depart2mentofMalaria,NationalInstituteofParasiticDiseases,ChineseCenterforDiseaseControlandPrevention,La2boratoryofParasiteandVectorBiology,MinistryofPublicHealth,WHOCollaboratingCentreforMalaria,SchistosomiasisandFilariasis,Shanghai200025,ChinaAbstractGenechiptechnology,developedinthelate1980s,hasbeendemonstratinggreatpoweronvariousareasoflifescience.ThistechnologyhasbeengraduallyappliedintheresearchofPlasmodium,Anoph2elesandmalariaanimalmodelssincetheaccomplishmentofwholegenomesequencingofPlasmodiumfalciparumandAnophelesgambiae.ThisreviewsummarisesthebasicprincipleofDNAmicorarrayanditsapplicationinmalariaresearch.KeywordsGenechip;Malaria;Plasmodium;Anopheles;Animalmodel:200025,,,(humangenomeproject,HGP)(modelorganism),,,,,2080(genechip),,[1],[2,3],,2005100[4],2002,[5,6],1DNA(DNAchip)DNA(DNAmicroarray),,,,,[7]South2ernNorthern,,,,,,,cDNA(complementDNA);(singlenucleotidepolymorphisms,SNP),,(la2boratoryonachip)[8]08120077344IntJMedParasitDis,July2007,Vol.34,No.4,,,,:[3],,(chargecoupleddevices,CCD)[3],,,,,,,,,[9],,,,,22.12000,,Hayward[10],DNA(shotgunDNA),,,,cDNA,LeRoch[11]2BenMamounIsokpehi[12,13]Bozdech[14]Young[15],Vontas[16],Ganesan[17]Dd2HB3Hall[18]Gissot[19]3D7F12,,,[20,21][22][23][24][25][26][27]2.2,2003,Kumar[28],(reactiveoxygenspecies,ROS),,2005DavidVontas[29,30],230,S(glutathione2S2transferase,GST)314A1(CYP314A1)P450s(dichloro2diphenyl2trichloroethane,DDT),P450CYP325A32005DanaMarinotti[31,32]cDNA(expressedsequencetag,EST),,2006Dis2sanayakeStrode[33,34],1/4,18120077344IntJMedParasitDis,July2007,Vol.34,No.4[35][36][37][38],2.3,,2004Sexton[39],400600,,2005Sarfo[40]cDNA,RANTES(regulatedonactivation,normalTcellexpressedandsecreted)CCR1CCR3CCR5mRNA,2006Delahaye[41]cDNA,31,2005Page[42],(interferongamma,IFN2)(tumornecrosisfactoralpha,TNF2)2006cDNA(CDCR),CDCR,BBurkitt’s[43]3,,,[1,3,7],:(1),,,;(2),,,;(3),;(4),,SNP,SNP,,SNP;(5),DNAcDNAEST,,,,,,,;,,,,,[44],,,[1]NguyenDV,ArpatAB,WangN,etal.DNAmicroarrayexperi2ments:biologicalandtechnologicalaspects.Biometrics,2002,58(4):7012717.[2]JaresP.DNAmicroarrayapplicationsinfunctionalgenomics.Ul2trastructPathol,2006,30(3):2092219.[3]HellerMJ.DNAmicroarraytechnology:devices,systems,andapplications.AnnuRevBiomedEng,2002,4:1292153.[4]WorldHealthOrganizationandUNICEF.Worldmalariareport2005.Geneva:WorldHealthOrganization,2005.11.[5]GardnerMJ,HallN,FungE,etal.Genomesequenceofthehu2manmalariaparasitePlasmodiumfalciparum.Nature,2002,419(6906):4982511.[6]HoltRA,SubramanianGM,HalpernA,etal.Thegenomese2quenceofthemalariamosquitoAnophelesgambiae.Science,28120077344IntJMedParasitDis,July2007,Vol.34,No.42002,298(5591):1292149.[7]BednarM.DNAmicroarraytechnologyandapplication.MedSciMonit,2000,6(4):7962800.[8]KallioniemiOP.Biochiptechnologiesincancerresearch.AnnMed,2001,33(2):1422147.[9]HacklH,SanchezCaboF,SturnA,etal.AnalysisofDNAmi2croarraydata.CurrTopMedChem,2004,4(13):135721370.[10]HaywardRE,DerisiJL,AlfadhliS,etal.ShotgunDNAmi2croarraysandstage2specificgeneexpressioninPlasmodiumfalci2parummalaria.MolMicrobiol,2000,35(1):6214.[11]LeRochKG,ZhouY,BatalovS,etal.Monitoringthechromo2some2intraerythrocytictranscriptomeofPlasmodiumfalciparumusingoligonucleotidearrays.AmJTropMedHyg,2002,67(3):2332243.[12]BenMamounC,GluzmanIY,HottC,etal.Co2ordinatedpro2grammeofgeneexpressionduringasexualintraerythrocyticdevel2opmentofthehumanmalariaparasitePlasmodiumfalciparumre2vealedbymicroarrayanalysis.MolMicrobiol,2001,39(1):26236.[13]IsokpehiRD,HideWA.IntegrativeanalysisofintraerythrocyticdifferentiallyexpressedtranscriptsyieldsnovelinsightsintothebiologyofPlasmodiumfalciparum.MalarJ,2003,2(1):38.[14]BozdechZ,ZhuJ,JoachimiakMP,etal.ExpressionprofilingoftheschizontandtrophozoitestagesofPlasmodiumfalciparumwithalong2oligonucleotidemicroarray.GenomeBiol,2003,4(2):R9.[15]YounJA,FivelmanQL,BlairPL,etal.ThePlasmodiumfalci2parumsexualdevelopmenttranscriptome:amicroarrayanalysisu2singontology2basedpatternidentification.MolBiochemParasitol,2005,143(1):67279.[16]VontasJ,Siden2KiamosI,PapagiannakisG,etal.Geneexpres2sioninPlasmodiumbergheiookinetesandearlyoocystsinaco2culturesystemwithmosquitocells.MolBiochemParasitol,2005,139(1):1213.[17]GanesanK,JiangL,RathodPK.Stochasticversusstabletran2scriptionaldifferencesonPlasmodiumfalciparumDNAmicroar2rays.IntJParasitol,2002,32(13):154321550.[18]HallN,KarrasM,RaineJD,etal.AcomprehensivesurveyofthePlasmodiumlifecyclebygenomic,transcriptomic,andpro2teomicanalyses.Science,2005,307(5706):82286.[19]GissotM,RefourP,BriquetS,etal.Transcriptomeof3D7anditsgametocyte2lessderivativeF12Plasmodiumfalciparumclonesduringerythrocyticdevelopmentusingagene2specificmicroarrayassignedtogeneregulation,cellcycleandtranscriptionfactors.Gene,2004,341:2672277.[20]WuY,WangX,LiuX,etal.Data2miningapproachesrevealhiddenfamiliesofproteasesinthegenomeofmalariaparasite.GenomeRes,2003,13(4):6012616.[21]HaywardRE.Plasmodiumfalciparumphosphoenolpyruvatecar2boxykinaseisdevelopmentallyregulatedingametocytes.MolBio2chemParasitol