高效液相色谱法测定果汁饮料中的糖精钠

[]yDetectionofsaccharinsodiumfromfruitjuicebeveragebyusinghighperformanceliquidphasechro-matography1WANGXiao-li,YANGDa-jin,LIYan-jun1(InstituteofNutritionandFoodSafetyofCh-ineseCenterforDiseaseControlandPrevention,Beijing100021,P1R1China)王小莉,杨大进,李燕俊中图分类号:R155159文献标识码:B文章编号:1009-9727(2005)07-1528-01[1],,,,,1111HP1100,();();1+9;1g/L1154g/L;:110mg/ml,1125,12,100%1135ml,1010ml,510ml125m1,1ml(1+9),,25ml,1min,2010ml2,,10~15ml1,5mlNaHCO3(1g/L),1min,,NaHCO31010ml,40~60e,,,pH7,,0145Lm[2~3]11411411HPLCHypersilODSC18,200mm@416mm,5Lm,11412g/L+=60+40:230nm,:110ml/min11413010201060110mg/ml,20Ll,HPLC,,3,,,,114141.220Ll,,2211+1154g/L(5+95)(15+85)(40+60)3,2,,,1,,,11HPLC21201010105011001150120g/L,20Ll,,6,,:Y=53162X(g/L)-17138,r=01999901004g/L11HPLC(g/L)(n)(s)(%)010160100063161010560100192157011060100243101011560100312121012060100593128213,,2(下转第1547页)1528CHINATROPICALMEDICINEVol15No17October2005200557y作者单位:中国疾病预防控制中心营养与食品安全所,北京1000211JinghuaJia[19]ExosExosHela,JNKERK1/2P38JNKExos,HelaJNKExosERKl/2P38,JNK1/2Ccas-pase,(),MAPKsMAPKs,,.:[1]MaXL,KumarS,GaoF,etal1Inhibitionofp38mitogen-activatedproteinkinasedecreasescardiomyocyteaoptosisandimprovescardiacfunctionaftermyocardialischemiaandreperfusion[J]1Circulation,1999,99(13):1685~1691.[2]TheodosiouA,AshworthA.MAPkinasephosphatases[J]1GenomeBiol,2002,26(7):3009.1~10.[3]SethiG,SodhiA.Activationofc-JunN-terminalkinaseisrequiredforultravioletB-inducedapoptosisofmurinepertonealmacrophagesinvitro[J]1JPhotochemPhotobiolB,2004,73(3):133~140.[4]KyriakisJM,AvruchJ.Mammalianmitogen-activatedproteinkinasesignaltransductionpathwaysactivatedbystressandinflammation[J]1PhysiolRev,2001,81(2):807~809.[5]EnslenH,RaingeaudJ,DavisRJ.SelectiveactivationofP38mitogen-act-ivatedprotein(MAP)kinaseisoformsbytheMAPkinasekinasesMKK3andMKK6[J]1JBiolChem,1998,273(3):1741~1748.[6]EckertRL,EfimovaT,BalasubramanianS,etal1p38Mitogen-activatedproteinkinasesonthebodysurface-afunctionforp38delta[J]1JInvestDermatol,2003,120(5):823~828.[7]JohnsonGL,LapadatR.Mitogen-activatedproteinkinasepathwaysmediat-edbyERK,JNK,andp38proteinkinases[J]1Science,2002,298(5600):1911~1912.[8]VanderHouvenVanOordtW,Diaz-MecoMT,LozanoJ,etal1TheMKK(3/6)-p38-signalingcascadealtersthesubcellulardistributionofhnRNPA1andmodulatesalternativesplicingregulation[J]1JCellBiol,2000,149(2):307~316.[9]LeeJC,KassisS,KumarS,etal,p38mitogen-activatedproteinkinasein-hibitors-mechanismsandtherapeuticpotentials[J].PharmacolTher,1999,82(2~3):389~397.[10]MancusoG,MidiriA,BeninatiC,etal,Mitogen-activatedproteinkinas-esandNF-kappaBareinvolVedinTNF-alpharesponsestogroupBstreptococci[J]1JImmunol,2002,169(3):1401~1409.[11]DahanS,BusuttilV,ImbenV,etal,EnterohemorrhagicEscherichiaco1iinfectionindutesinterleukin-8productionviaactivationofmitogen-act-ivatedproteinkinasesandthetranscriptionfactorsNF-kappaBandAP-1inT84cells[J]1Infectlmmun,2002,70(5):2304~2310.[12]HuhJE,KangKS,ChaeC,etal,Rolesofp38andJNKmitogen-activat-edproteinkinasepathwaysduringcantharidin-inducedapoptosisinU937cells[J].BiochemPharmacol,2004,67(10):1811~1818.[13]FettucciariK,FetriconiI,BartoliA,etal,lnvolvementofmitogen-act-ivatedproteinkinasesinGroupBStreptococcus-inducedmacrophageapoptosis[J].PharmacolRes,2003,47(4):355~362.[14]EllingtonJK,ElhofyA,BostKL,etal.Involvementofmitogen-activatedproteinkinasepathwaysinStaphylococcusaureusinvasionofnormalos-teoblasts[J]1Infectlmmun,2001,69(9):5235~5242.[15]TsengHJ,ChanCC,ChanEC.SphingomyelinaseofHelicobacterpylori-inducedcytotoxicityinAGSgastricepithelialcellsviaactivationofJNKk-inase[J]1BiochemBiophySResCommun,2004,314(2):513~518.[16]SlomianyBL,SlomianyA.DisruptioningastricmucinsynthesisbyHel-icobacterpylorilipopolysaccharideinvolvesERKandp38mitogen-activat-edproteinkinaseparticipation[J]1BiochemBiophysResCommun,2002,294(2):220~224.[17]WatababeK,YilmazO,NakhjiriSF,etal,Associationofmitogen-act-ivatedproteinkinasepathwayswithgingivalepithelialcellresponsestoPor-phyromonasgingivalisinfection[J]1lnfectlmlnun,2001,69(11):6731~6737.[18]SlomianyBL,SlomianyA.Porphyromonasgingivalislipopolysaccharidein-terfereswithsalivarymucinsynthesisthroughinduciblenitricoxidesyn-thaseacbvationbyERKandp38kinase[J]1BiochemBiophysResCom-mun,2002,297(5):1149~1153.[19]JinghuaJia,MouniaAlaoui-E1-Azher,MarieChow,etal,c-JunNH2-TerminalKinase-MediatedSignalingisessentialforPseudomonasaeruginosaExos-Inducedapoptosis[J].Infectlmmun,2003,71(6):3361~3370.收稿日期:2005-06-27(上接第1528页)2(n=6)(g/L)(g/L)(g/L)(%)1011501010115685201150105011919030115011001246953,,,,[4],512,0~0105g/L:[1]1[S].GB/T5009128-1996.[2],,,1HPLC[J]1:,2005,41(1):2~41[3],.[J].,1991,1(2):100~102.[4]1[J]1,2005,15(4):4281收稿日期:2005-07-011547200557CHINATROPICALMEDICINEVol15No17October2005