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当前位置:首页 > 医学/心理学 > 药学 > 雄性SD大鼠两种取血方法血清生化及内分泌系统指标变化比较_李连珍
29920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.29No.9Sep.2011SD1211111.1000292.450002SD。SDGLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACCOR、T3、T4、TSH、E2、T。GLU、COR、TP、ALB、GOT、GPT、UA、CK、CKMB、LDH、LAC。SD。R332A1673-7717201109-1984-03ComparisonofTwoBloodCollectionMethodsonBiochemistryandEndocrineinMaleSDRatsLILian-zhen12LIUXiao-qing1ZHANGQian1LINZhi-jian1ZHANGBing11.BeijingUniversityofChineseMedicineBeijing100029China2.HenanAgriculturalUniversityZhengzhou45002HenanChinaAbstractObjectiveTocomparetheeffectsoftwobloodcollectionmethodsanestheticanddecapitatedonbiochemis-tryandendocrineinmaleSDrats.MethodsMaleSDratsweredividedintotwogroups.Bloodsampleswerecollectedaf-teranesthetizedbyintraperitonealinjectionofpentobarbitalsodiumanddecapitationrespectively.SerumparametersofGLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACweredetectedbyautomaticbiochemicalana-lyzer.TheendocrinesystemindexofserumE2TCORT3T4TSHweredeterminatebyradio-immunity.ResultsSerumGLUCORTPALBGOTGPTUACKCKMBLDHLAClevelsweredifferencebetweenthesetwobloodcollectionmethods.ConclusionTherearesignificantinfluencesonbiochemistryandendocrineparametersbetweenthesetwobloodcollectionmethods.Itshouldpaymoreattentionontheeffectsofbloodcollectionmethodinanimalexperimentandchooserationalbloodcollectionmethodwiththeexperimentalrequirements.KeywordsRatPentobarbitalsodiumDecollationBloodbiochemistryEndocrinehormone2011-04-029732007CB5126051979-。1959-、。SD。。。。。1-23、、、。11.1SD20240~250gSCXK2007-0001。SPF。1.2Sigma200704152%。GLU80125L041TG71129K021TC911171ITP003051AALB001201AALT71114J811AST80125L041CRE912141KABUN489129920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.29No.9Sep.201180313B811UA80213A811LDH80318C011LAC80318C011CK9020911ACKMB71116K211。。1.3JOUANxh6080CX4。1.410。3312h2%45mg/kg。SD。1~2h3000r/min15min。1.5GLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACCX4GLUTGTCTPALBGOTGPTIFCCCREBUNUVUA-PODCKCKMBDGKCLDHLAC。COR、T3、T4、TSH、E2、T。1.6SAS8.0珋x±stP<0.05。22.11GLUP<0.01TPALBUAP<0.01TGTC。1n=10珋x±sGLUmmol/LTGmmol/LTCmmol/LTPg/LALBg/LUAμmol/L5.02±0.970.60±0.130.84±0.1764.35±2.8027.19±1.4957.37±14.753.57±0.800.89±0.451.02±0.2673.32±2.4730.31±1.2090.23±26.08P<0.01。2.22n=10珋x±sGOTU/LGPTU/LBUNμmol/LCREμmol/L177.70±33.1773.40±10.084.98±0.44125.32±46.19290.10±55.87113.10±24.344.99±0.84138.85±47.17P<0.01。2GOTGPTP<0.01BUNCRE。2.33LAC、LDH、CKCKMBP<0.01。3n=10珋x±sLDHU/LLACumol/LCKU/LCKMBU/L2060.30±406.876.61±0.83561.80±149.771078.10±287.062478.90±321.668.59±1.192491.89±266.536893.67±669.92P<0.01。4n=10珋x±sCORng/mLE2pg/mLTng/mLT4ng/mLT3ng/mLTSHuIU/mL10.84±5.8033.74±12.025.74±4.2478.51±17.970.56±0.132.83±0.385.56±3.4626.19±19.062.83±1.8191.59±18.370.58±0.192.59±0.50P<0.052.44CORP<0.05E2、T、T3、T4、TSH。3。。、、、、、、。。SD。3.1。589129920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.29No.9Sep.20114-7、、。、、GLU、TP、ALBUATGTCGOTGPTBUNCRELAC、LDH、CKCKMB。。3.2--、----。COR、E2、T、T3、T4、TSH。8-9。CORE2、T、T3、T4、TSH。3.3。①、10。。11SD、。。②12。③、。、、GLU、TP、ALB、UA、GOT、GPT、LAC、LDH、CK、CKMB、COR。。1.J.200724119-20.2.SDJ.2008272299-3023.、、SDJ.2007178496-498.4.J.200616265-68.5.J.200616269-72.6.J.200616273-77.7.J.200616278-80.8.J.1997185474—476.9.、J.200577763.10.J.200919851-54.11.SDJ.200818143-45.12BalcombeJPBarnardNDSanduskyC.LaboratoryroutinescauseanimalstressJ.LabAnimSci2004436檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪42-51.“”、。。。“”。。。。、。500。“”。。。6891
本文标题:雄性SD大鼠两种取血方法血清生化及内分泌系统指标变化比较_李连珍
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