☆RT-PCR常用的内参引物

一、RT－PCR常用的内参引物内参基因名称引物引物最佳退火扩增基因库序列号引物名称序列位置Tm温度C长度HumanactinbetaF305ctgggacgacatggagaaaa305-32452.3BC002409R868aaggaaggctggaagagtgc868-84952.659.4564F1379agcgagcatcccccaaagtt1379-139857.3R1663gggcacgaaggctcatcatt1663-164456.354285RatactinbetaF18cacccgcgagtacaaccttc18-3754.5NM_031144R224cccatacccaccatcacacc224-20554.460.4207F694gagagggaaatcgtgcgtgac694-71454R1146catctgctggaaggtggaca1146-112753.257.1452MouseactinbetaF91atatcgctgcgctggtcgtc91-11057.5NM_007393R607aggatggcgtgagggagagc607-58857.860.4517F1566gtccctcaccctcccaaaag1566-158554.5F1831gctgcctcaacacctcaaccc1831-181154.455.7266humanGAPDHF369agaaggctggggctcatttg369-38855.6BC004109R626aggggccatccacagtcttc626-60755.157.5258F66aggtcggagtcaacggatttg66-8652.5R597gtgatggcatggactgtggt597-57852.657532RatGAPDHF50cagtgccagcctcgtctcat50-6955BC059110R644aggggccatccacagtcttc644-62555.158.3595F1029acagcaacagggtggtggac1029-104854.4R1280tttgagggtgcagcgaactt1280-126154.857.7252MouseGAPDHF306aggccggtgctgagtatgtc306-32554M32599R835tgcctgcttcaccaccttct835-81654.658.7530F49ggtgaaggtcggtgtgaacg49-6855.1R281ctcgctcctggaagatggtg281-26254.556.8233Rat18SrRNAF1865tgcggaaggatcattaacgga1865-188658.6M11188R2164agtaggagaggagcgagcgacc2164-21435959.3300human18SrRNAF1565cagccacccgagattgagca1565-158458.3M10098R1816tagtagcgacgggcggtgtg1816-179758.359253mouse18SrRNAF56aggggagagcgggtaagaga56-7554.4K013364R296ggacaggactaggcggaaca296-2775462241二、常用的beta-actin内参引物大鼠432bpA001ratactinfTCAGGTCATCACTATCGGCAATA002ratactinrAAAGAAAGGGTGTAAAACGCA大鼠150bpA003ratactinfCCCATCTATGAGGGTTACGCA004ratactinrTTTAATGTCACGCACGATTTC人205bpA005hactinfTGACGTGGACATCCGCAAAGA006hactinrCTGGAAGGTGGACAGCGAGG人434bpA007hactinfCGGGAAATCGTGCGTGACA008hactinrTGGAAGGTGGACAGCGAGG小鼠263bpA009mactinfGAGACCTTCAACACCCCAGCA010mactinrATGTCACGCACGATTTCCC小鼠446bpA011mactinfGAGACCTTCAACACCCCAGCA012mactinrCCACAGGATTCCATACCCAA上海闪晶分子生物科技有限公司地址：邮编：201109联系：市场部电话：[phone]E-mail:[e-mail]网址：