微流控纸芯片的加工技术及其应用1 (1)

PＲOGＲESSINCHEMISTＲYDOI10.7536/PC130616http//．progchem．ac．cnProgressinChemistry2014261167～177*＊＊310058、、、。、、、、、、。。O652.91005-281X201401-0167-112013620131020131225*No．20890020No．2012BAI13B06TheworkwassupportedbytheNationalNaturalScienceFoundationofChinaNo．20890020andtheNationalKeyTechnologyＲ＆DProgramofChinaNo．2012BAI13B06＊＊[e-mail]fMicrofluidicPaper-BasedChipsandTheirApplications*JiangYanMaCuicuiHuXianqiaoHeQiaohong＊＊InstituteofMicroanalyticalSystemsDepartmentofChemistryZhejiangUniversityHangzhou310058ChinaAbstractMicrofluidicpaper-basedchipanalysisisaburgeoningmicrofluidictechnique．Itpossessesgreatpotentialsforapplicationinclinicaldiagnosisfoodqualitycontrolaswellasenvironmentalmonitoringduetoitsattractivefeaturessuchaslow-costeasy-to-fabricateeasy-to-useandportable．Itsimportanceandutilityarewidelyacknowledgedandextensiveresearchhasbeenconductedinthepastseveralyears．Thispapermainlyaimstoreviewthedevelopedtechniquesforfabricationofmicrofluidicpaper-basedchipsincludingUVphotolithographywaxprintingplasmatreatmentinkprintinginkjetetchingplottingscreenprintingflexographyprintingandlasertreatmentandsoon．Thedetectionmethodsforthemicrofluidicpaper-basedchipanalysisandapplicationsofmicrofluidicpaper-basedchipsarealsoreviewed．Keywordsmicrofluidicpaper-basedchipsfabricationdetectionmethodsapplicationsContents1Introduction2Paperchoices3Fabricationtechniquesofmicrofluidicpaper-basedchips3.1UVphotolithographytechnique3.2Waxprintingtechnique3.3Plasmatreatmenttechnique3.4Inkprintingtechnique3.5Inkjetetchingtechnique3.6Plottingtechnique3.7ScreenprintingandflexographyprintingＲeview·168·ProgressinChemistry2014261167～177technique3.8Waxdippingtechnique3.9Lasertreatmenttechnique3.10Othertechniques4Detectionmethodsinmicrofluidicpaper-basedanalyticaldevices4.1Colorimetricdetection4.2Electrochemicaldetection4.3Chemiluminescenceandelectrochemilumine-scencedetection5Applicationsofmicrofluidicpaper-basedanalyticaldevices5.1Clinicaldiagnosis5.2Foodqualitycontrol5.3Environmentalmonitoring6Conclusionandperspective1。、、/“”lab-on-papermicrofluidicpaper-basedanalyticaldevicesμPADs。2007Whitesides1μPADs。、、1。、/、1～3、4～6、7、892D10113D。2、。0.07～1mm。3。1、412DNA13。4。。5。。、14～16μPADs。。2、。、、。。、Whatman117。4DNA13。Lu4。Al-Tamimi18、Kleenex。Kleenex。、Whatman1。。31～3101119～30、4～61731～48、4950、751、5253、89、3954、5556、5758592014261167～177·169·2D。11103D。2D、。、、、、、、。3.1、/。SU-8。Whitesides1SU-8“”。。。1。SU-8。WhitesidesSC21SU-8、PGMEA23。。Haller2。PoNBMA。。PoNBMApH=8。1SU-81Fig．1ProcedureforUVphotolithographyfabricationofmicrofluidicpaper-basedchipwithSU-81self-assemblingmonolayersSAMs60、61。He3。OTSOTSOTS130°。254、185nmUV/O3/2。、、。3.2Lin45Whitesides6。Lin45、150°C。Whitesides6。、Ｒeview·170·ProgressinChemistry2014261167～17723Fig．2Schematicdiagramforthefabricationofmicrofluidicpaper-baseddevicebyOTSself-assemblingandUV/O3-patterning3。3.3AKD。Shen4950AKD。AKD100℃5min。AKDAKD。。3.42010Shen7AKD。AKD100℃8minAKD。。3.5Abe5253。311.0%～1.8%2h210～203。、。。352Fig．3Fabricationprocessoftheinkjet-printedmicrofluidicimmunosensingstrip523.6PDMS。2008Whitesides8PDMS。PDMSPDMS。。2012Li9。。、、、、。3.7394654。Henry54。2014261167～177·171·。。。Olkkonen55。/。、。3.8Laiwattanapaisal5859waxdipping。4“---”120～130℃。。。458Fig．4SchematicdiagramforfabricationprocessoftheμPADusingthewaxdippingmethod583.92011Ziaie57。CO2。SiO2。。3.10Zhang62。。2D1。2D11203135361044463D。Whitesides112D3D。Crooks102D3D。44、、。4.15～1117192022272833～35464853555658596263。。、Photoshop、ImageJ。Martinez22。。pH。、、。、、。KlasnerＲeview·172·ProgressinChemistry2014261167～1771Table1Summaryandcomparisonoftechniquesforfabricating2Dmicrofluidicpaper-basedchipsfabricationtech-niquespatterningagentspatterningprinciplespatterningapproachesadvantagesdisadvantagesrefphotolithographySU-8physicalblockingofporesinpapertwo-stepfabricationhighresolutionofhydro-philic-hydrophobicpatternrequiresexpensiveequip-mentfabricationprocessistediousdevicesarevulner-abletobending1101119～30PoNBMAphysicaldepositionofreagentonfibersur-facetwo-stepfabricationhighresolutionofhydro-philic-hydrophobicpatternrequiresexpensiveequip-mentdevicesarevulnera-bletobending2OTSchemicalmodificationoffibersurfacetwo-stepfabricationhighresolutionofhydro-philic-hydrophobicpatternpatterningreagentwithlowcostdevicesareflexiblerequiresexpensiveequip-ment3waxprintingwaxphysicaldepositionofreagentonfibersur-faceone-stepfabricationproducesmassivedeviceswithsimpleandfastfabri-cationprocesslow-costrequiresexpensivewaxprinterslowresolutionofhydrophilic-hydrophobicpattern4～61731～48plasmatreat-mentAKDchemicalmodificationoffibersurfacetwo-stepfabricationpatterningagentwithlowcostfabricationprocessissimplerequiresdifferentmasksforcreatingdifferentmicroflu-idicpatternssubstrateiseasilytobeoveretched4950inkjetprintingAKDchemicalmodificationoffibersurfaceone-stepfabricationmassproductionwithsimpleandfastfabricationprocessrequiresexpensivewaxprinters751inkjetetchingpolystyrenephysicaldepositionofreagentonfibersur-facetwo-stepfabricationprintingbio/chemicalsens-ingreagentsdirectlycreationofmicrofluid