1株铜绿微囊藻降解菌的分离鉴定及其溶藻特征

:042727104(2010)0120094205:2009209228:(08ZZ03);(20060246024);(08ZR1401300);:(1957),,,E2mail:[e-mail];,,E2mail:[e-mail].11,1,2,1,1(1.,200433;2.,250101):,1H1.H116SrDNA.H1:,1d71.6%,7d80.48%.,.:;;;:X131.2:A,,,[1]..[223],,[425].(Algicidalbacteria),[6],,,.1924,Geitler,[7].,,[8].1(Alexandriumcatenella)(Alexandriumtamarense)[9].Mitsutani,Chattonellaantiqua[10].[11].[12].1H1,NP,H1.11.11.1.1(Microcystisaeruginosa),FACHB905.,25,3000lx,14h/10h.1.1.2BG11:1000mLNaNO3:1.500g;MgSO47H2O:49120102()JournalofFudanUniversity(NaturalScience)Vol.49No.1Feb.20100.075g;Na2SiO39H2O:0.058g;Ferricammoniumcitrate:0.006g;Na2CO3:0.02g;K2HPO4:0.04g;CaCl22H2O:0.036g;Citricacid:0.006g;EDTA:0.001g;A5solution+Co:0.001g.A5solution:1000mLH3BO3:2.86g;ZnSO47H2O:0.22g;Na2MoO42H2O:0.039g;MnCl24H2O:1.81g;CuSO45H2O:0.079g.LBLB.LB:950mL10g,5g,NaCl10g,5mol/LNaOH(0.2mL)pH7.0.1L.0.1MPa20min;:100mLLB1.5g,pH7.0,0.1MPa20min.1.1.3,FACHB905,11,chla10001200g/L,106107cells/mL,.1.21.2.1,2,1,.,27F(5p2AGAGTTTGATCMTGG2CTCAG23p)1512R(5p2ACGGCTACCTTGTTACGACT23p),16SrDNA,().1.2.2H1H1LB,OD4201.0(109mL-1),50mL400mL(chla),5,,50mLLB,250r/min,35.chla1Tab.1Themonitoringmethods(chla)/(gL-1)[13](T2N)/(mgL-1)[14](T2P)/(mgL-1)[14]TNTP(4000r/min,30min)TNTP.chla=100%(chla-chla)/chla,T2N=100%(T2N-T2N)/T2N,T2P=100%(T2P-T2P)/T2P.1.22.1H1H1LB,,,,,;LB.H1,L2A2L2L27,L216,.H1PCR1.5kb,:591:1GenebankEU594550.16SrDNA99.7%.,H1.2.2H1H1(chla)(T2N),(T2P)(T2N),(T2P)13.1,H1,chla2d,24h,,chla,71.6%,2d,chla,80.48%.2,(P0.01),24h,(P0.05).1d,(P0.05),24d,,100%.4d.,,.3,(P0.05).,.69()4933.1,,[15].,,.,,2,chla,,.,..,,,,.,,.3.2[16],.rRNA,.rRNA,,,,16SrRNA,5SrRNA,,23SrRNA(Mr2.9kb),16SrRNA,.16SrDNA.16SrDNA,,.3.3H1,,,,H1,2448h,.,,.,,H1,.:[1]BettinaC,StefanH,DietrichDRCyanobaterialtoxins:removeduringdrinkingwatertriatmint,andhumenrishaeeseement[J].EnvironmentalHealthPefepectives,2000,108(Suppl):1132120.[2],,.[J].,2001,4:14216.[3]LambertTW,HolmesCFB,HrudeySE.Adsorptionofmicrocystinbyactivecarboninfullscalewatertreatment[J].WaterResearch,1995,29(8):184521854.[4]LeeS,KatoJ,TakiguchiN,etal.InvolvementofanextracellularproteaseinalgicidalactivityofthemarinebacteriumPseudoalteromonassp.strainA28[J].AppliedandEnvironmentalMicrobiology,2000,66(1):433424339.[5]ImamuraN,MotoikeI,ShimadaN,etal.Anefficientscreeningapproachforanti2Microcystiscompoundsbasedonknowledgeofaquaticmicrobialecosystem[J].JournalofAntibiotic,2001,54(6):5822587.[6].[D].:,2006,5.[7]CaiolaMG,PellegriniS.LysisofMicrocystisaeruginosabyBdellovibrio2likebacteria[J].JPhycol,1984,20(1):4712475.[8],.[J].,1981,7(3):3772384.[9],,.(Pseudoalteromonassp.)[J].,2008,27(3):56260.[10]MitsutaniA,YamasakiI,KitaguchiH,etal.Isolationofanalgicidalmarinebacteriumwithactivity791:1againsttheharmfuldinoflagellateHeterocapsacircularisquama(Dinophyceae)[J].Phycologia,2001,40(3):28622911.[11],,.[J].,2008,35(2):1712177.[12],,.T5[J].:,2007,46(3):3082311.[13],,.a[J].,2006,18(5):5502552.[14].[M].4.:,2002,10.[15],,,.DC2L14[J].,2009,15(1):1062109.[16],,.[J].,2002,15(5):43246.Isolation,IdentificationandLyticCharacterizationofaBacteriumThatLyseMicrocystisaeruginosaWANGXiang2rong1,HUHuan1,MURui2min2,XUBo1,FANZheng2qiu1(1.DepartmentofEnvironmentalScienceandEngineering,FudanUniversity,Shanghai200433,China;2.SchoolofMunicipal&EnvironmentalEngineering,ShandongJianzhuUniversity,Jinan,250101,China)Abstract:OnestrainofalgicidalbacteriaH1wasisolatedfromtheetiolatedMicrocystisaeruginosa.Itsphysiologicalbiochemistrycharacteristicandtheresultsof16SrDNAsequenceanalyzestronglysuggestedthatalgicidalbacteriaH1belongstoBrevibacillussp.ItsalgicidalcharacteristiconMicrocystisaeruginosawastested.ResultsshowedthatthealgicidalbacteriaH1hadagoodremovaleffectonMicrocystisaeruginosa.Theremovalratestochlorophyllareached71.6%of1dand80.48%of7d.Duringalgicidalprocess,thephenomenonthatnitrogenandphosphorweretransferredfrominsidetooutsideofalgaecellwasalsonoticed.Keyword:Microcystisaeruginosa;algicidalbacteria;algicidalcharacteristic;chlorophyll89()49