A2O工艺强化反硝化除磷体系中微生物特性分析王晓莲

　2007,13(3):401～407　　ChinJApplEnvironBiol=ISSN1006-687X　2007-06-25　:2005-09-02　　:2006-09-05＊(No.50628808)(No.50521140075)　SupportedbyOverseasYouthScholarCooperationProjectofNSFC(50628808)andtheKeyInternationalCooperationProjectoftheNationalNaturalScienceFoundationofChina(No.50521140075)＊＊　Correspondingauthor(E-mail:[e-mail])A2/O＊王晓莲　王淑莹　彭永臻＊＊(　　100022)　　,A2/O,-β(PHB)(Poly-P),,.,,PHB,,,1～1.5μm、;,PHB,,,0.5～1μm1.0～1.5μm;,PHB,,,1.0～1.5μm,,.,.812　A2/O;;;PHB;CLC　X703CharacteristicsofSludgeCommunityinA2/OProcesswithDenitrifyingPhosphateRemovalPerformance＊WANGXiaolian,WANGShuyingPENGYongzhen＊＊(KeyLaboratoryofBeijingWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100022,China)Abstract　Inordertowellunderstandthemechanismofnitrogenandphosphorusremovalandoperationalstateindenitrifyingphosphorusremovalsystem,thisstudyaimedtoanalyzethecharacteristicsofactivatedsludgeinquasi-stateA2/OprocessbymeansofdirectstainingtogetherwithSEManalysis,andobservethevariationofPHBandpolyphosphategranuleinthecellsatdifferentphases.Theresultsshowedthatattheanaerobicphase,thePHBconcentrationinthecellandorthophosphateconcen-trationoftheanaerobiceffluentobviouslyincreased,thepoly-Pconcentrationinthecelldecreased,andthecucciandbacilliwithasizeof1.0～1.5μmweredominantandformedgrapycellularcluster.Attheanoxicphase,thePHBconcentrationandorthophosphateconcentrationdecreasedsignificantly,theamountofpoly-Pincreased,andtheellipticalcucciof0.5～1μmandspheralbacilliof1.0～1.5μmweredominant.Attheaerobicphase,thePHBandorthophosphateconcentrationalmostreducedtozero,thepoly-Pincreasedsignificantly,cucciappearedsingleorinpair,andthecontourofspherewasblurandal-mostdisappeared.Incontrast,onlynitrogenremovalsystemhadnosuchchanges.Fig8,Ref12Keywords　anaerobic-anoxic-oxicprocess;gramstaining;SudanBlackstaining;PHBstaining;scanningelectronicmicroscopyCLC　X703　　,、,,.A2/O,、,,.,(BPR).,“”,(PAOs)(VFA)(Glycogen)-β(PHB),(PP)(ATP);,PHB,.(1%～3%),12%,.[1,2],,,,.,30%50%,,VFA,,.1　A2OFig.1　SchematicdiagramofA2Oprocess1.;2.;3.;4.;5.1;6.2;7.1;8.2;9.1;10.2;11.3;12.4;13.;14.;15.;16.;17.;18.;19.;20.1.Feed;2.Tank;3.Peristalticpump;4.Stirrer;5.Anerobic1;6.Anerobic2;7.Anoxic1;8.Anoxic2;9.Aerobic1;10.Aerobic2;11.Aerobic3;12.Aerobic4;13.Settlingtank;14.Airflowmeter;15.Airpressure;16.Effluent;17.Diffuser;18.Recirculationflow;19.Recycleflow;20.Ex-cesssludge　　(EBPR)SBR,,EBPR,[3,4].,,PHB,,,A2/O,、.1　A2/O1.1　A2/O1,——.,COD、NH+4-NPO3-4300mgL-1、50mgL-110mgL-1,NaHCO3pH6.5～7.5.,,..1.2　1.2.1　　　4:(1);(2)(,PP);(3)(β-,PHB);(4),.2,2,.1.2.2　　　[5],TCOD、SCOD、NH-4-N、NOa-3-N、NO-2-N、TN、PO3-4-P、pH、TP、、DO、ORP、MLSS、MLVSS,.PHBSmolders[6].1.2.3　　　(1)PHB():①,0.3%,10min,,.②,.③,0.5%1～2min,,.,.(2)():①,3～5min,.②,1min,.③.,.(3):①,1min,.②1min,,.③95%,30s,,,.④,30s,.⑤,,.(4):①,4℃1.5h;②,3,10min;③,50%,70%,80%,90%,10～15min;100%3,10～15min;④,100%:=1∶1,,15min;⑤HCP-2(HITACHI);⑥,;⑦,IB-5(Giko)1500nm();⑧.2　、,,,,.EBPR,,.,PHB.2.1　2,TIN、PHBPO4402　　　　　　　　　　ChinJApplEnvironBiol　　　　　　　　　　　　　　　　　13——,,,.,、PHB2.6～2.8、50%～60%2.8～3.2,93.4%97.5%,,,.2　TIN,PO4PHBFig.2　TIN,PO4,PHBprofilesatdifferentphasesANA2:2;ANX2:2;AER5:5ANA2:Anaerobic2;ANX2:Anoxic2;AER5:Aerobic52.2　Kortstee[7],,.,,,.3,a、b、c、d、、.PP,.3-a,,,,,3,-300mV.(3-b),、,、,,,,,,-100～-150mV.(3-c),100mV,,,,,0,3　Fig.3　Poly-Pstainingatdifferentphases403　3:A2/O　　.3,,,,45%,.,,,[8].(3-d),,.2.3　PHB,,.4,a、b、c、d、、(PHB).PHB,.4-a,,PHB,.PHB,,.4-b,,,,,,,;PHB,,,,2PHB.4-c,,、、,,PHB,PHB,,,,[9],.4-dPHB,PHB,,,PHB[10].4　PHBFig.4　PHBstainingatdifferentphases2.4　,.EBPR(PAOs,polyphosphateaccumulatingorganisms)(GAOs,glycogenaccumulatingorganisms).Seviour[11],PHB,PAOs,404　　　　　　　　　　ChinJApplEnvironBiol　　　　　　　　　　　　　　　　　13GAOs,FISH,5-a5-b.6a、b、c、d、、.6-a,、1.0～1.5μm,5　(a)(b)EBPRGAOmixPAOmixFig.5　FishimageofbiomassfromapoorlyperformingEBPR(a)andalab-scaleEBPRreactor(b)(a)FISH,GAOmix,EUBmix,7μm;(b)FISH,PAOmix,EUBmix,10μm(a)Competibacterappearyellow(GAOmix)andotherbacteriaappeargreen(EUBmix).Bar=7μm.(b)Accumulibacterappearmagenta(PAOmix)andotherbacteriaappearblue(EUBmix).Bar=10μm6　Fig.6　Imageofelectronicscanningmicroscopyatdifferentphases405　3:A2/O　　PHB,.、.[3],γ-,2.5～3.5μm,.Kong[12],、312,GAOs,;PAOs,GAOs,.6-b,,0.5～1μm1.0～1.5μm,,,.6-c,,,1.0～1.5μm,,,,,PHB.1.0～1.5μm,0.5～1μm,PHB,7-a7-b.(8),2.6-d,,6-c,0.3μm,0.5～0.8μm,1.6～2.0μm.,.,,.7　Fig.7　TypicalgranulesludgestainingintheA2/Oprocess8　Fig.8　DistributionofgranulesludgeintheA2/Oprocess3　EBPR,,,A2/O.,,,PHB,1～1.5μm,3;,,,PHB,,0.5～1μm1.0～1.5μm,45%;,,,,PHB,.,、PHB,0.3～0.8μm、1.6～2.0μm.,,,.References1　WangHY(),ZhouYX(),JiangJY().Bac-teriacommunityanditscharacteristicsinenhancedbiologicalphosphorusremoval.ActaMicrobiol(),2005,32(1):118～1222　RobertJS,TakashiM,MotoharuO.Themicrobiologyofbiologicalphosphorusremovalinactivatedsludgesystems.FEMSMicrobiolRev,2003(27):99～1273　RenSY(),XiaoT().Stainingmethodsofmultipolyerin406　　　　　　　　　　ChinJApplEnvironBiol　　　　　　　　　　　　　　　　　13polyphosphateaccumulatingorganisms.SciOcean(),2005,29(1):59～634　,..:,1998.224～2285　APHA.StandardMethodsfortheExaminationofWaterandWastewater.19thed.WashingtonDC,USA:AmericanPublicHealthAssociation/Ameri