ABR中厌氧颗粒污泥的微生物学特性

ABR,,(,710055):(ABR),ABR,F420,ABR,1#,,2#3#,,4#;1#,,2#3#4#,ABRF420,,F420:;;:X703.1:C:1000-4602(2004)10-0049-03:(01JK187)MicrobiologicalCharacteristicsofAnaerobicGranularSludgeinABRXUJin2lan,HUANGTing2lin,WANGZhi2ying(SchoolofEnvironmentalandMunicipalEngineering,XianUniversityofArchitectureandTechnology,Xian710055,China)Abstract:Itisimportanttounderstandmicrobiologicalcharacteristicsofanaerobicgranularsludgeinanaerobicbaffledreactor(ABR)forthesakeofculturinggranularsludge.Therefore,anobservationandanaly2sisonthecompositionofanaerobicgranularsludgeineachseparationchamberofABRwereconductedbyusingscanningelectronmicroscope,andspecificmethanogenicactivityandthecoenzymeF420contentweredeter2mined.TheresultindicatesthatthedisparityofmicrobialcompositionissignificantamongtheseseparationchambersofABR.AcidogenicbacteriaarepredominantonthesurfaceofgranularsludgewhilemethanogenicbacteriaarepredominantininteriorofgranularsludgeinchamberNo.1.AsforchamberNo.2andchamberNo.3,therearenoabsolutelypredominantbacteriaandtherearemultipleandcomplicatedbacteriacolonies.InchamberNo.4thepredominantbacteriaofgranularsludgeisSohngeniimethanogenicbacteria.InchamberNo.1themethanogenicactivityarousedbyglucoseoraceticacidislowerthanthatbypropionicacid.Conversely,inchamberNo.2,No.3,No.4,themethanogenicactivitybyusingglucoseoraceticacidallishigherthanthatbyusingpropionicacid.CoenzymeF420contentofgranularsludgeinABRincreasesprogressivelyalongsepara2tionchambers(accordingtotheorderofnumberedchambers),whichconformswiththeorderofmethanogenic942004Vol.20CHINAWATER&WASTEWATERNo.10activityincrease.Inotherwords,thecoenzymeF420alsocanbeusedforindicatingthemethanogenicactivityofgranularsludgeinABR.Keywords:ABR;anaerobicgranularsludge;microbiologicalcharacteristicABR,,[1],1111,15.84LABRABR(1)4,,(331)ABR24h,COD2500mg/L1Fig.1Schematicdiagramofexperimentalset2up112238dABR,;F420[2];[2]2211,,1#,,,,,;(0.5m,3m),H2,[3]1#,,COD2#(),,2#H2,1.5d;,,(Methanosarcina)5d,(Methanosaeta)SRT12d[4]ABR,,,3#2#,,,(1#2#),4#,ABR(pH),212,,11Tab.1MaximumspecificmethaneproductionratesofgranularsludgewithdifferentsubstratesmL/(gd)1#31745752#16937963#128241184#1355550:,,Lettinga[5](SMPA),ABRSMPA,052004Vol.20No.10,,,,,,[6]1,1#,1#,ABR2#3#,4#,213F420F420(2),F420,,4#F4201#,1#,4#,F4202F420Tab.2ContentofF420ingranularsludgeofeachchamberg/(gd)1#2#3#4#F42078256026621154103ABR(pH),1#,H2,2#3#,,4#ABR,,,ABRF420,,F420:[1].[M].:,2002.[2],,.[M].:,1990.[3],.[M].:,1986.[4]GradyJrCPleslie,GlenTDaigger,HenryCLim.[M]..:,2003.[5]LettingaG.Advancedanaerobicwastewatertreatmentinthenearfuture[J].WatSciTech,1997,35:5-12.[6],,.(ABR)[J].,2002,4:362-365.:[phone]E-mail:[e-mail]:2004-04-13,:16.7km,:DN300DN600,:PE,:2000,:20042005:,:9.5km,:DN1200DN1400,,:2100,:200452005:,:()152004Vol.20No.10