CANON反应器运行稳定性及温度冲击的影响付昆明

3310201210ENVIRONMENTALSCIENCEVol．33No．10Oct．2012CANON1222221.1000442.100124CANONCANON．CANON35℃±1℃pH7.39～8.013.7～5.1h1aTN1.8kg·m3·d－1TN65.09%TN81.65%．ANAMMOXAOBANAMMOXNO－2-N．35℃CANONCANON50℃AOB1ANAMMOXCANON．CANONX703.1A0250-3301201210-3507-062011-11-072012-04-11101101402508780031981～[e-mail]CANONReactorandTemperatureImpactFUKun-ming12ZHANGJie2CAOXiang-sheng2LIDong2MENGXue-zheng21.KeyLaboratoryofUrbanStormwaterSystemandWaterEnvironmentMinistryofEducationSchoolofEnvironmentandEnergyEngineeringBeijingUniversityofCivilEngineeringandArchitectureBeijing100044China2.KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentalRecoveryEngineeringCollegeofArchitectureandCivilEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractInordertostudylong-termeffectofcompletelyautotrophicnitrogenremovalovernitriteCANONreactorperformancestabilitywasinvestigatedbyusingsyntheticinorganicammonia-richwastewaterasrawwaterwithacontinuousflowCANONreactor．Bothperformancesofshort-cutnitrificationandANAMMOXwerestableformorethanoneyear．Undertheconditionthatinnertemperatureat35℃±1℃pH7.39and8.01andhydraulicretentiontime3.7-5.1htheaveragetotalnitrogenremovalloadwas1.8kg·m3·d－1andtheaverageandmaximumtotalnitrogenremovalefficiencywere65.09%and81.65%respectively．UndersuddenlowtemperatureconditionsbothANAMMOXbacteriaandAOBwereinhibitedhowevertheANAMMOXbacteriawereinhibitedmorewhichcausedhighlyaccumulatednitrite．Whentemperatureincreasedto35℃asnormaltheperformanceofCANONreactorrecoveredsoonwhichmeanslowtemperatureimpactwillhavenosignificantinfluenceonstability．Whenthetemperaturereachedmorethan50℃theactivityofANAMMOXbacteriawascompletelydestroyedsohightemperaturemustbeavoidedthoughAOBcanrecoveredtonormalinoneweek．KeywordsCANONprocessanaerobicammoniaoxidationshort-cutnitrificationnitritestabilitytemperatureCANONcompletelyautotrophicnitrogenremovalovernitriteanaerobicammoniumoxidationANAMMOX1CANON．NH+4-NN2ammonia-oxidizingbacteriaAOBANAMMOX2CANON、、、．CANONANAMMOXNH+43CANON4、56、7NO－2-NNO－3-N8ANAMMOX．CANONANAMMOX．CANONDOI:10.13227/j.hjkx.2012.10.037332910CANON1a2CANON．11.13.85L1．2cm×2cm×2cm1g·cm－350%2～3mm．．XMT-10235℃±1℃．pHHI931700pH7.39～8.01．1.2.3.4.5.6.7.8.9.10.pH11.1Fig．1Experimentalapparatusandprocessschemechart1.2CANONCANONSBRANAMMOXANAMMOX210dTN1.22kg·m3·d－1ANAMMOXNO－2-N、NH+4-NNO－3-N1∶1.36∶0.2211ANAMMOX1∶1.32∶0.2612ANAMMOX．1.3NH4Cl、NaHCO3、NaClK2HPO4NH+4-N300～500mg·L－1NO－2-N0～10mg·L－1NH+4-NNO－3-N3～5mg·L－1PO3－4-P2mg·L－1pH7.5～8.5．1.4CANON35℃±1℃210～300d14～42m3·m3·d－118L·d－15.1h300d1.5～2.0m3·m3·d－125L·d－13.7h．245d491d21℃15℃548d50℃．1.5NH+4-N13NO－2-NN-1--13NO－3-N14pHpHTestr30pHHI2400TN．TN=NH+4-N+NO－2-N+NO－3-N2TN2NH+4-N、NO－2-N、NO－3-NTN3～6．2.12.1.1CANON23NH+4-NNH+4-N．4210～271dNO－2-N20mg·L－160mg·L－1271dNO－2-N30mg·L－15NO－3-NNO－3-N28.88mg·L－1300dNH+4-N76.72%NH+4-N96.99%NO－2-N21.81805310CANON2TNFig．2Variationsofaerationrateandnitrogenremovalloadduringoperationperiod3NH+4-NFig．3VariationsofNH+4-Nduringoperationperiod4NO－2-NFig．4VariationsofNO－2-Nduringoperationperiodmg·L－1NO－3-N27.62mg·L－1．2.1.2NO－2-N/NOx－-N5NO－3-NFig．5VariationsofNO－3-NduringoperationperiodCANONAOBANAMMOXNH+4-NANAMMOXNO－2-NNO－2-N/NOx－-N．dTNTNTNdTNANAMMOXN2dNO－3-NNO－3-N①ANAMMOXCO2NO－3-N15②nitrite-oxidizingbacteriaNOBNO－2-NNO－3-N．CANONNOBdNO－3-NANAMMOXCO2NO－3-NANAMMOX16NO－3-NN20.26∶1.02dNO－3-N/dTN=0.26/1.02×2=0.127．NOBdNO－3-NdTNNOBANAMMOXNO－2-NdNO－3-N/dTNdNO－3-N/dTN0.127CANON．0．548～571ddNO－3-N/dTN0.1270.09dNO－3-N/dTN0.1270NOB905333dNO－3-N/dTN0.127＜0．CANON35℃±1℃pH7.39～8.013.7～5.1h．2.1.3ANAMMOXANAMMOXTN．2TN17TN．218dNH+4-N96%TNNO－2-N63.41mg·L－1ANAMMOXANAMMOXNO－2-N．300dTN66%6．300dTN1.4kg·m3·d－11.9kg·m3·d－1．300dTN1.66TNFig．6VariationsofTNduringoperationperiodkg·m3·d－1TN2.32kg·m3·d－1TN1.8kg·m3·d－1ANAMMOX．TN81.65%TN65.09%TN．TN①CANONTN89%10②NH+4-NNO－2-N18TN③TN．TN．2.2ANAMMOXAOB．TNANAMMOXAOBANAMMOXNH+4-NAOBNH+4-NANAMMOXNH+4-N．ANAMMOXNH+4-N∶N21∶1.0216ANAMMOXNH+4-N=dTN/1.02×2=dTN×0.49．2.2.1CANONAOB1．1ANAMMOXAOBTable1DifferentlowtemperatureimpactonANAMMOXbacteriaandAOB/d/℃TN/mg·L－1ANAMMOX/%NH+4-N/mg·L－1ANAMMOXNH+4-N/mg·L－1AOBNH+4-N/mg·L－1AOB/%23935243．3359．24287．56119．23168．338．012452199．18203．4448．60154．8448735298．0982．99341．80146．06195．7425．624911550．72170．4424．85145．5921℃ANAMMOX59.24%AOB8.01%15℃ANAMMOX82.99%AOB25.62%．NO－2-N4．AOBANAMMOX015310CANONANAMMOXCANON19．2CANON2～6．、ANAMMOX．2.2.2548d36℃10h50℃．548dCANON．549dNH+4-N5.74%CANONTN8.74%NO－3-N2.6mg·L－1ANAMMOXNH+4-N9.97%AOB．1NO－2-N554dNO－2-N159.84mg·L－1NH+4-N45.66%1AOBNH+4-N140.06mg·L－1AOBTN0ANAMMOX571dTNANAMMOXCANON1620ANAMMOX43℃．CANON．3135℃±1℃pH7.39～8.013.6～5.1hNH+4-N300～500mg·L－1ANAMMOXCANONTN81.65%．2ANAMMOXAOBANAMMOXNO－2-N．350℃CANONANAMMOXAOB1．1ThirdKASliekersAOKuenenJGetal．TheCANONsystemcompletelyautotrophicnitrogen-removalovernitriteunderammoniumlimitationinteractionandcompetitionbetweenthreegroupsofbacteriaJ．SystematicandAppliedMicrobiology2001244588-596．2KimuraYIsakaKKazamaF．EffectsofinorganiccarbonlimitationonanaerobicammoniumoxidationanammoxactivityJ．BioresourceTechnology201110264390-4394．3V