O工艺同步反硝化除磷脱氮研究

23220106　　　　　　JournalofZhongkaiUniversityofAgricultureandEngineering　　　　　　　　Vol.23,No.2June,2010　:2009-09-18:(2007Z3-E0061).:(1984-),,,,.　　＊:E-mail:[e-mail]2/O1,　2,2＊(1.仲恺农业工程学院农学院,广东广州510225;2.仲恺农业工程学院环境科学与工程学院,广东广州510225):,(Denitrifyingphosphatebacteria,DPB),.,6～10mg/L,30～35mg/L,25～30mg/L,(Chemicaloxygendemand,COD)150～250mg/L,、、COD0.65mg/L、12.6mg/L、3.8mg/L34mg/L,;DPB(Acinetobactor)、(Enterobacteriaceae)、(Pseudomonas)、(Staphylococcus)(Paracoccus),(A.junii);167d,DPB94%;,.:MA2/O;A2/O;;;:X78　　　　　:A　　　　　:1674-5663(2010)02-0011-06InvestigationofMA2/OprocessdenitrifyingphosphorusandnitrogenremovalsimultaneouslyGUXue-ting1,LIUHui2,ZHOUKang-qun2＊(1.CollegeofAgronomy,ZhongkaiUniversityofAgricultureandEngineering,Guangzhou510225,China;2.CollegeofEnvironmentalScienceandEngineering,ZhongkaiUniversityofAgricultureandEngineering,Guangzhou510225,China)Abstract:Theself-designedsimultaneousphosphorusandnitrogenremovalsystemnamedMA2/Owerestud-ied.Thedenitrifyingphosphatebacteria(DPB)inthesystemwasisolatedandidentified.TheresultsshowedthatwhentheconcentrationofTP,TN,NH+4-NandCODintheinfluentwere6～10mg/L,30～35mg/L,25～30mg/Land150～250mg/L,respectively,theconcentrationofTP,TN,NH+4-NandCODinfinalef-fluentintheexperimentalprocesswere0.65mg/L,12.6mg/L,3.8mg/Land34mg/L,respectively.ThemainDPBstrainsinthesystemwereisolatedandidentifiedtobeAcinetobactor,Enterobacteriaceae,Pseudo-monas,StaphylococcusandParacoccus.TheproportionofDPBwas94%onthe167thday.Testsshowedthatthedevicecouldbeappliedtohandlethestandardofwateruptothenationallevel.Theresultsofenrichmentshowedthatthetypesofbacteriawereconcentrated,withA.juniifoundasakindofDPBforthefirsttime.Keywords:MA2/Oprocess;A2/Oprocess;denitrifyingphosphateaccumulation;denitrifyingphos-phatebacteria;simultaneousremovalofphosphorusandnitrogen　　,、.--(Anaerobic-Anoxic-Oxic,A/A/OA2/O)、A/A/O,(Chemicaloxygendemand,COD).,CO2,.,[1-4].,O2,(Denitrifyingphosphatebacteria,DPB),.DPB,,,、,.(Sequen-cingbatchreactor,SBR),,A2/O[5-7].A2/O、,A2/O,、,--(Modifiedanaerobic-anoxic-oxic,MA2/O),,,.1　1.1　1.1.1　试验装置　1.A2/O、,,DPB,50%COD.,,DPB,.()(),,(Oxidation-reductionpotential,ORP)-80mV..DPB,DPB;,(Dis-solvedoxygen,DO),.2,(Suspendedsubstance,SS),DPB.1m3/d,(Hydraulicretentiontime,HRT).,,、.:①V()V()=1.51;②(Food/Microorganism,F/M)0.88～1.32KgBOD/(m3·d);③(Mixedliquorsuspendedsolids,MLSS)1800～2000mg/L;④1.0m3/d;⑤pH7～7.5;⑥8～13d.　1.Anaerobictank;2.Settletank;3.Biofilmoxidationtank;4.Facultativedeoxytank;5.Denitrifyingphosphorustank;6.Aerobictank;7.Settletank;8.Pump;9.Magneticstirrer;10.ActivatedsludgewithP;11.Backflowsludge;12.Residualsludge1　MA2/OFig1　DeviceandschematicdiagramofMA2/Oprocess1.1.2　供试材料　A2/O,,(Biochemi-caloxygendemand,BOD)80～120mg/L,COD150～250mg/L,pH7～7.5,SS100～160mg/L,NH+4-N25～30mg/L,30～35mg/L,PO3-4-P6～10mg/L.,DPB.[8],、.:5g,KH2PO40.25g,MgSO4·7H2O0.5g,CaCl2·H2O0.2g,(NH4)2SO42.0g,(FeCl31.5mg,CuSO40.03mg,KI0.18mg,MnCl20.12mg,ZnSO40.12mg,CoCl30.15mg,10mg,1000mL)1.0mL,1000mL,pH7.0,121℃15min.1.1.3　菌种来源　,.12　　23　1.2　1.2.1　同步脱氮除磷装置的启动调试　,NH+4-NNO-3-N,DPBNO-3-N.,ORP80～200mV.90%,DPB,17.HRT210min※20min※132min※12min※160min※10min※20min,2000～2500mg/L,ORP-150～-220mV,ORP-100～-120mV,6.,,DPB,.,DPB,.1.2.2　静态释/聚磷效果试验　3h,1L,,m()m()=21.1.2.3　细菌的分离与鉴定　[9],.,、、,[10].1.2.4　指标分析　NO-3-N752C();NO-2-N、NH+4-N、TPMLSSα-、、[11];COD5B-1COD;ORP()SUNTEXTS-2;pHH198127pH.2　2.1　2.1.1　启动阶段　,,3,,;NH+4-N92%;NO-2-N(NH+4-NNO-2-N)66.2%2.8%;,NO-3-N(NH+4-NNO-3-N),13.3%94.6%(1).NO-3-N.,,[12],,NO-2-N.,,NO-3-NNO-2-N,(ORP121～193mV)、,NH+4-NHNO2,DPB.1　Table1　Changeofbacteriaamountandnitrateduringstart-upstageItem　　Time/d1631466690NH+4-NNH+4-Nremoval%78.3±0.7492.5±0.2693.7±3.8389.1±1.0995.8±0.298.7±0.12NO-2-NNO-2-Ngenerationrate/%66.2±0.6273.1±0.6257.4±0.4638.9±0.6115.3±0.472.80±0.15NO-3-NNO-3-Ngenerationrate/%13.3±0.5315.7±0.3639.6±0.0651.0±2.2572.5±4.894.6±0.7Nitrobacteria/CFU/mL5.7×104±1071.6×104±2395.0×104±0.07.3×105±106141.1×106±91385.0×106±0.0Nitrosobacteria/CFU/mL1.5×106±260762.5×106±438911.7×105±286455.7×105±41544.5×105±28051.2×105±176813　2　　　　　,:MA2/O　　　2.1.2　反硝化聚磷菌的富集阶段　PO43--PNO-3-N10d12.1%48.5%64d54.7%91.5%,32d2.1×105CFU/mL75d1.2×106CFU/mL.167d,PO43--PNO-3-N95.4%96.1%,,4.3×106CFU/mL(2、2).2　PO43--PNO-3-NFig2　ChangeoftheremovalrateofPO43--PandNO-3-Nduringenrichmentstage　　3d1.1×105CFU/mL167d4.3×106CFU/mL.、,,DPB-β-(Poly-β-hydroxybu-tyrate,PHB),PHB.,(Adenosine-triphosphate,ATP)ATP,,.32d,MLSS,(2).,ORP,,.MA2/ODPB,A2/O,.2　Table2　ChangeofDPBamountandMixedliquorsuspendedsolids(MLSS)duringenrichmentstageItemTime/d33275103129167DPB/(CFU/mL)1.1×1052.1×1051.2×1062.5×1062.1×1064.3×106MLSS/(mg/L)2200～25001375～15001460～16001575～17501700～18001780～1880　　1d,0.6mg/L(),DPBA2/O,(3d1.1×105CFU/mL).PO43--P,0.6mg/L8.2mg/L,0.6mg/L11.5mg/L(3)。DPBP.2.1.3　体系的稳定阶段　40d.39d,(Totalphosphorus,TP)0.65mg/L,(Totalnitrogen,TN)12.6mg/L,NH+4-N3.8mg/L,COD34mg/L(3).TP、TN、NH+4-N、COD.,TP1mg/L,TN15mg/L,NH+4-N5mg/L,COD50mg/L,[13],./,PO43--P1.44g/3　PO43--PFig3　ChangeofPO43--Pduringenrichmentstage(kg·h).DPB94%,DPB.2.2　3h,6mg/L27.7mg/L,PO43--P2.07g/(kg·h)(4).,4.5h,0.84mg/L,14　　23　3　Table3　Changeofmai