PCR技术在水体微生物检测中的应用

PCR!200092(PCR)PCRPCRDNAX830.2A1003-6504(2006)09-0103-03(PolymeraseChainReactionPCR)1985(ASHG)DNAPCRPCRDNA1PCRPCRDNADNAdNTPDNADNADNA[1]PCRDNADNADNADNAPCRDNADNA4dNTP94!DNADNADNA35pHTagDNADNA53PCRPCRDNA2PCRPCRPCRAtmar[2]PCRChung[3]PCR50!Brooks[4][e-mail]103299200699;990PCR90Tsen[5]16srRNAPCR091E..colicell/1OomL0[6]9PCR09,,09PCRDNA990semenova[7]RAPD-PCRRandomAmpli!fiedPolymorphicDNA-PCR9DNA-PCR09PCR090PCR909903PCR99990PCR16srDNA916srDNA90Emilie[8]PCR-DGGE(PCR-denaturinggra!dientgelelectrophoresis9PCR)099;9DNAPCRDGGE0PCR909Nico[9]PCR-DGGE990PCR0ZHANGDan[1o]OLAND(),0PCR-DGGE9OLAND090Alina[11]09904PCRPCR-DGGE0909999ssCODBOD9[1]09PCRDGGE9099[13]0[14]PCR-DGGE99091O4aOaaO[15]aaOaPCRa\\OaaOaPCRa\PCRaaOaDNAaPCRaPCROaaOaPCRaaPCRaa[16]OaaaO!PCRaOaaDNAaOaaOaaaOPCRaaO#[1]GerardMuyzer,NielsBRamsing.Molecularmethodstostudytheorganizationofmicrobialcommunities[J].Wat.Sci.Tech.,1995,39(8):1-9.[2]AtmarRL,NeillFH,RomaldeJL,etal.DetectionofNorwalkvirusandHepatitisAvirusinshellfishtissueswiththePCR[J].Appl.Environ.Microbiol.,1995,61:3014-3018.[3]ChungH,JaykusLA,SobseyMD.Detectionofhumanentericvirusinoystersbyinvivoandinvitroamplifica!tionofnucleicacids[J].Appl.Environ.Microbiol.,1996,62:3772-3778.[4]HilaryABrooks,RichardMGersberg,ArunKDhar.De!tectionandguantificationofhepatitisAvirusinseawaterviareal-timeRT-PCR[J].JournalofVirologicalMethods,2005,127(2):109-118.[5]TsenHY,LinCK,ChiWR.Developmentanduseof16SrRNAgenetargetedPCRprimersfortheidentificationofEscherichiacolicellsinwater[J].Appl.Micobiol.,1998,85:554-560.[6],,.PCR[J].,2004a20(9)193-95.[7]SemenovaSK,RomanovaEA,RyskovAP.Geneticdif!ferentiationofhelmintheswiththearbitraryprimedpoly!merasechainreaction[J].Ecol.Genetics,1996,32:304-309.[8]EmilieLyautey,BndicteLacoste,Lo#cTen-Hage,etal.Analysisofbacterialdiversityinriverbiofilmsusing16SrDNAPCR-DGGE:methodologicalsettingsandfinger!printsinterpretation[J].WaterResearch,2005,39:380-388.[9]NicoBoon,WimDeWindt,WillyVerstraete,etal.Evalua!tionofnestedPCR-DGGE(denaturinggradientgelelec!trophoresis)withgroup-specific16SrRNAprimersfortheanalysisofbacterialcommunitiesfromdifferentwastewatertreatmentplants[J].MicrobiologyEcology,2002,39:101-112.[10]ZhangDan,ZhangDe-min,LiuYao-ping,etal.Commu!nityanalysisofammoniaoxidizerintheoxygen-limitedni!tritationstageofOLANDsystembyDGGEofPCRampli!fied16SrDNAfragmentsandFISH[J].JournalofEnviron!mentalScience,2004,16(5):838-842.[11]AlinaCCole,JohnWShanahan,MichaelJSemmens,etal.Preliminarystudiesonthemicrobialcommunitystructureofmembrane-aeratedbiofilmstreatingmunicipalwastewater[J].Desalination,2002,146:421-426.[12],,.[J].,2003a22(1)116$18.[13],,,.PCR-DGGE[J].a2004a25(6)174$79.[14]SigingXia,FengWang,YigangFu,etal.Biodiversityanal!ysisofmicrobialcommunityinthechem$bioflocculationtreatmentprocess[J].BiotechnologyandBioengineering,2005,89(6):656-659.[15]SigingXia,YanShi,YigangFu,etal.DGGEanalysisof16SrDNAofammonia-oxidizingbacteriainchemical-bio!logicalandchemicalcoagulationsystems[J].EnvironmentalBiotechnology,2005,69(1):99-105.[16].PCR[J].,2004a24(7):59-64.(2005-ll-07Z2006-0l-05)PCRa105~~PCR技术在水体微生物检测中的应用作者：戴睿，夏四清，DAIRui，XIASi-qing作者单位：同济大学污染控制与资源化研究国家重点实验室,上海,200092刊名：环境科学与技术英文刊名：ENVIRONMENTALSCIENCE&TECHNOLOGY年，卷(期)：2006,29(9)被引用次数：7次参考文献(16条)1.王建龙PCR技术检测水体中大肠菌群的研究[期刊论文]-中国生物工程杂志2004(07)2.SiqingXia;YanShi;YigangFuDGGEanalysisof16SrDNAofammonia-oxidizingbacteriainchemical-biologicalandchemicalcoagulationsystems[外文期刊]2005(01)3.SiqingXia;FengWang;YigangFuBiodiversityanalysisofmicrobialcommunityinthechem-bioflocculationtreatmentprocess[外文期刊]2005(06)4.NicoBoon;WimDeWindt;WillyVerstraeteEvaluationofnestedPCR-DGGE(denaturinggradientgelelectrophoresis)withgroup-specific16SrRNAprimersfortheanalysisofbacterialcommunitiesfromdifferentwastewatertreatmentplants[外文期刊]2002(2)5.EmilieLyautey;BénédicteLacoste;LoicTen-HageAnalysisofbacterialdiversityinriverbiofilmsusing16SrDNAPCR-DGGE:methodologicalsettingsandfingerprintsinterpretation20056.SemenovaSK;RomanovaEA;RyskovAPGeneticdifferentiationofhelmintheswiththearbitraryprimedpolymerasechainreaction19967.马颖;龙腾锐;方振东PCR技术检测饮用水中大肠杆菌[期刊论文]-中国给水排水2004(09)8.TsenHY;LinCK;ChiWRDevelopmentanduseof16SrRNAgenetargetedPCRprimersfortheidentificationofEscherichiacolicellsinwater[外文期刊]1998(3)9.HilaryABrooks;RichardMGersberg;ArunKDharDetectionandquantificationofhepatitisAvirusinseawaterviareal-timeRT-PCR[外文期刊]2005(02)10.ChungH;JaykusLA;SobseyMDDetectionofhumanentericvirusinoystersbyinvivoandinvitroamplificationofnucleicacids[外文期刊]1996(10)11.AtmarRL;NeillFH;RomaldeJLDetectionofNorwalkvirusandHepatitisAvirusinshellfishtissueswiththePCR[外文期刊]199512.王峰;傅以钢;夏四清PCR-DGGE技术在城市污水化学生物絮凝处理中的特点[期刊论文]-环境科学2004(06)13.夏四清;杨殿海;高廷耀化学生物絮凝工艺处理城市污水试验研究[期刊论文]-上海环境科学2003(01)14.AlinaCCole;JohnWShanahan;MichaelJSemmensPreliminarystudiesonthemicrobialcommunitystructureofmembrane-aeratedbiofilmstreatingmunicipalwastewater[外文期刊]2002(1/3)15.ZhangDan;ZhangDe-min;LiuYao-pingCommunityanalysisofammoniaoxidizerintheoxygen-limitednitritationstageofOLANDsystemby