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pH王新为,孔庆鑫,金敏,宋农郑金来,古长庆,李君文(,,300050) :探讨硝化细菌最佳工作条件,为应用和生产提供依据。通过人工调节液体培养基的pH值和充气,检测氨氮和亚硝酸盐氮转化率。分离的亚硝酸细菌在转化氨氮为亚硝酸盐氮的过程中产生大量强酸,可使pH值下降到5.6或更低,必须不断加入碱性物质给予中和。实验表明,培养过程中不加碱组氨氮转化率为20%左右,加碱组氨氮转化率则可达到99%左右。而硝酸细菌则产酸不明显,pH维持在7.8~8.1。培养基连续充气可使氨氮在亚硝酸细菌的作用下去除率增加89%左右,亚硝酸盐氮在硝酸细菌作用下去除率增加85%左右。硝酸细菌与亚硝酸细菌均为好氧菌,不断曝气给予充足的溶解氧,pH值维持在7.5~9.0,才能更好地发挥其硝化作用。 : ; ; :R117 :A :1001-5248(2003)05-0319-04 〔1〕。,,,,,。,,,〔2〕。,,,,pH、〔3,4〕。,,,。1 1.1 (1):、、、:“”(No.2001AA214191,2002AA601240)(No.0231807111):(1956~),,,。。,0.5g。pH7.5~9.0。(2):,、、PCR。(3):。(4):METTLERTOLEDO320。(5)/:85,。1.2 :2L1750mL,30~50mL,103·mL-1。〔5〕。。2 2.1 pH 。pH7.9~9.0,6.0。1,120mg·L-1,(23℃),pH5.6。·319· 200310215 JPrevMedChinPLA October2003 Vol.21No.5DOI:10.13704/j.cnki.jyyx.2003.05.003(10%),pH,。2.2 2,pH,16d10%13mL,120mg·L-11.5mg·L-1,99%。,pH,,18%20%。1116,pH6.0,,。,,pH,。NH+4pHNH3。〔6〕,6000mg·L-1Ca(OH)2,pH10.5,20℃,。2.3 。3,;,,,。1 pH pH1d6d11d16dG-34()7.56.835.725.59G-34()7.517.487.277.55G-46()7.446.505.865.73G-46()7.457.057.007.20()7.497.096.736.262 (mg·L-1)1d6d11d16dG-34()123.28123.56103.73101.21G-34()120.34107.0851.511.62G-46()128.10121.33107.64102.35G-46()121.28104.9752.331.33()134.32143.27124.35115.843 (mg·L-1)1d6d11d16dG-34()4.059.0111.508.51G-34()4.1016.5060.02100.92G-46()4.2010.5110.508.75G-46()4.2121.0064.02105.01()0.0020.0090.010.0172.4 pH 4,,,;,24d,359~127mg·L-1。,·320· 200310215 JPrevMedChinPLA October2003 Vol.21No.5。,,pH,,,pH(5)。,,。2.5 ,。〔7〕,1mg,4.27mg。,(1750mL)(0.5L·min-1)。,,pH7.5~9.3。6,7d,G-46,8%,4 (mg·L-1)1d10d15d16d20d21d24dQ-7112.1125.010.0359.050.02127.500.04I-1488.021.830.0360.120.03115.110.0395.0380.0286.0087.0196.1096.0177.005 pH pH1d10d15d20d24dQ-78.017.787.857.847.89I-148.077.837.917.897.968.067.797.917.977.966 (mg·L-1)1d4d7d(mg·L-1)1d4d7dG-46 117.3661.673.047.2548.0190.00 125.85122.41115.637.0014.0120.51Q-7 105.1262.000.42 110.21128.0295.0013.5mg·L-1;,97%,83mg·L-1。Q-7,14%,99%。 ,pH,,,,。,。pH,。。:〔1〕 .〔M〕.:,1990.125~126·321· 200310215 JPrevMedChinPLA October2003 Vol.21No.5〔2〕 , ,,.〔M〕.:,2001.96~98.〔3〕 ,.〔J〕.,1997,18(2):18〔4〕 ,,,.〔J〕.,1999,5(1):64.〔5〕 .〔S〕.:,2001.323.〔6〕 ,.〔J〕.,2000,26(9):44.〔7〕 .〔M〕.:,1999.190~192.EFFECTOFPHANDAERATIONONNITRIFICATIONOFNITROBACTERIAWANGXin-Wei,KONGQing-Xin,JINMin,SONGNongZHENGJin-Lai,GUChang-Qing,LIJun-Wen(InstituteofHealthandEnvironmentalMedicine,AcademyofMilitaryMedicalSciences,Tianjin,300050,China) ABSTRACT:ObjectiveTostudythebestconditionsofnitrificationfornitrobacteriasoastoprovidethebasisfornitrobacteriaproductionandapplication.MethodsAmmonianitrogen(NH3-N)andnitritenitrogen(NO2-N)trans-formationratesweremeasuredbyajustingthepHandgivingaerationtotheculturemedium.ResultsAmmonia-oxidiz-ingbacteriaproducedmuchacidasitconvertedNH3-NtoNO2-N,whichcouldmakepHlowerthan5.6.Ammoniatransformationratewasabout20%withoutalkaliaddition,whereastheratewasabout99%withalkaliaddition.Nitrite-oxidizingbacteriadidnotproduceacidevidently,andtherangeofpHwas7.8~8.1.TheeliminationratesofNH3-Nbyammonia-oxidizingbacteriaandNO2-Nbynitriteoxidixingbacteriawereincreasedbyabout89%and85%respec-tivelyaftercontinuousaerationtotheculture.ConclusionAmmonia-oxidizingbacteriaandnitrite-oxidizingbacteriawereaerobicbacteria.NitrificationofnitrobacteriawouldbemoreefficientwhenaerationwascontinuouslygiventosupplysolubleoxygenandtherangeofpHwaskeptat7.5~9.0. KEYWORDS: nitrobacteria; nitrification; environmentpollution(:2002-07-25;:2003-03-07)《》2004《》2004,。 :18-105,A4,,。5.00,6, 30.00。 。,。 : 300050 1 ·322· 200310215 JPrevMedChinPLA October2003 Vol.21No.5
本文标题:PH值与曝气对硝化细菌硝化作用的影响王新为
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