SBR中反硝化聚磷菌的培养驯化研究杨文婷

32SBR1212*1.2.215011、。-----40dPO43--P0.2mg/L95%NH4+-N8mg/L90%。NO3--NPO43--P。SBRX172X703.1A1003-6504(2009)08-0006-03EnrichmentandCultivationofDenitrifyingPhosphorusRemovingBacteriainSequencingBatchReactorYANGWen-ting12SHENYao-liang12*1.JiangsuProvincialKeyLaboratoryofEnvironmentalScienceandEngineering2.SchoolofEnvironmentalScienceandEngineeringSuzhouUniversityofScienceandTechnologySuzhou215011ChinaAbstractDenitrifyingphosphorusremovingbacteriawasenrichedandculturedusingactivatedsludgefromawastewatertreatmentplantwithdomesticwastewaterasrawwaterinasetofsequencingbatchreactorSBR.Resultsshowedafterthesystemwasoperatedundertheconditionofinfluent-idle-anoxic-settling-dischargefor40daysremovalofPO43--PandNH4+-Nwas95%and90%respectivelywhentheeffluentconcentrationwasbelow0.2mg/Land8mg/Lrespectively.TherelationshipbetweenNO4+-NconsumptionandPO43--PremovalwaslinearwhichindicatedthatenrichmentandcultivationofdenitrifyingphosphorusremovingbacteriainasetofSBRwasfeasible.KeywordsmunicipalwastewatersequencingbatchreactorSBRdenitrifyingphosphorusremovingbacteriaremovalofnitrogenandphosphorus、。。O2。O2NO3--NO2。[1]。“”[2]。SBR。11.1SBR20cm50cm14L。、35℃。8h。1。1.2SBR1。1.3COD、NH4+-N、PO43--P、NO3--N、pH。2008-06-272008－08－3150678107(B)[e-mail]*[e-mail]。EnvironmentalScience&Technology32820098Vol.32No.8Aug.2009822.1-38h4min--210min-180min-60min-。6LMLSS3000~4000mg/L5d。3d。20d95%。2.2-2h。Kuba[3]C/N3.4100%。C/N5.93.4。2min--120min-210min－60min-。38h4LDPB[4]KNO325mg/L30min。30dPO43--P90%NO3--NNO2--N、[5]。33.1PO43--P2--PO43--P。210d90%15d95%PO43--P0.11mg/L。PAOPHBPAOPHB[6]。-PO43--P45%15d90%PO43--P0.17mg/L。-PO43--P-PHA。PO43--P3。PO43--PPO43--P。。VFAPHANO3--N1Table1RawwaterqualityindexCOD(mg/L)185.91~324.86NH4+-N(mg/L)31.39~54.98PO43--P(mg/L)2.59~4.84pH6.8~7.2NO3--N(mg/L)0.35~0.55SBR32。3.2NH4+-N4NH4+-N95%NH4+-N48%。KNO3N2。18dNH4+-N85%40d90%。NO3--NNH4+-N。3.3NO3--NPO43--PNO3--NPO43--P5。NO3--NPO43--PDPB[7]5KNO3PO43--PPO43--P=1.2138NO3--NR2=0.98281mg/LNO3--N1.2138mg/LPO43--PNO3--NPO43--P。41--DPB。2SBR35℃PO43--P0.2mg/L95%NH4+-N8mg/L90%DPB。3NO3--NPO43--P。[][1].[J].,20056920-24.ZhaoQingYuanLin-jiangWangZhi-yingetal.Studyoncharacteristicsofdenitrifyingdephosphorization[J].Tech－niquesandEquipmentforEnvironmentalPollutionControl20056920-24.inChinese[2].SBR[J].2007298608-611.LvJuanChenYin-guangGuGuo-wei.Studyofnitrogenandphosphorusremovalbyasequencingbatchreactorwithintermittentaeration[J].EnvironmentalPollution＆Control2007298608-611.inChinese[3]KubaTvanLoosdrechtMCMHeijnenJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyin－tegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WatRes19963071702-1710.[4].[J].200629714-15.LiuHuiZhouKang-qunLiuKai-qietal.Denitrifyingphosphorusremovalbacteriawithnitriteaselectionacceptoranditsaffectingfactors[J].EnvioronmentScience＆Technol－ogy200629714-15.inChinese12322A1DO2.011.2mg/LA124h90%DOA1。3C/NA1C/N＞5。A1。4A1。[][1]RobertsonLAKuenenJG.Aerobicdenitrificationacontr-oversyrevived[J].ArchMicrobiol19841395351-356.[2]LARobertsonJGKuenen,RKleijintjens.Aerobicdeni－trificationandheterotrophicnitrificationbyThiosphaerapan－totropha[J].AntonievanLeeuwenhoek1985514445-448.[3]DPatureauNBernetJPDelgenès,etal.Effectofdis－solvedoxygenandcarbon-nitrogenloadsondenitrificationbyanaerobicconsortium[J].AppliedMicrobiologyandBiotechnology2000544535-542.[4].[J].200425175-78.ZhangYa-guangFangBai-shanMinHangetal.Char-acteristicsandphylogeneticanalysisofastrainofaerobicdenitrifier[J].JournalofHuaqiaoUniversity200425175-78.inChinese[5].N2O[J].200831526-29.LiuJing-jingWangPingMaJie-feng.Anaerobicdenitrifierwithlowlevelsproductionofnitrousoxide[J].Environ-mentalScience&Technology200831526-29.inChinese[6].X31[J].200533742-46.MaFangWangHong-yuZhouDan-danetal.Denitrifica-tioncharacteristicsofanaerobicdenitrifyingbacteriumpeudomonaschloritidismutansstrainX31[J].JournalofSouthChinaUniversityofTechnology200533742-46.inChinese[7].[J].200026417-20.LvXi-wuLiFengDaosenYoupingetal.Astudyofaerobicdenitrificationindenitrificationofammonianitrogenwastewater[J].Water&WastewaterEngineering200026417-20.inChinese[8].[J].200525377-81.YuAi-rongLiYouYuJi-an.DenitrificationofanewlyisolatedBacillusstrainW2anditsapplicationinaquaculture[J].JournalofMicrobiology200525377-81.inChinese[9].[J].2008355679-684.FanLi-rongHuangShao-binYangJunetal.Separatingandstudyingoftheaerobicdenitrifyingbacteriafrombiofilter[J].Microbiology2008355679-684.inChinese[10]WilsonLPBouwerEJ.Biodegradationofaromaticcom－poundsundermixedoxygen/denitrifyingconditionsare－view[J].JournalofIndustrialMicrobiology&Biotechnolo－gy199718116-130.8[5].———[J].2005282106-109.YangGuo-jingLiXiao-mingZengGuang-mingetal.Simultaneousbiologicalphosphorusandnitrogenremovingtechnology———denitrifyingdephosphatation[J].Envioron－mentScience＆Technology2005282106-109.inChi－nese[6].———[M]..2006.ShenYao-liangWangBao-zhen.NewTechnologiesforBiologicalWastewaterTreatment———TheoryandApplica－tion[M].SecondEdition.BeijingChinaEnvironmentalSci－encePress2006.inChinese[7].