UASB处理高浓度硫酸盐废水启动过程污泥特性变化杨丽平

UASB1,2,2,2,2,3,2,2＊(1.,　330045;2.,　510070;3.,　510275):UASB243d..1.82mm0.99mm;、,.N2H2S,N260mL·min-1,1.51mm;N2100mL·min-1,;N2.(MLVSS),33.59g·L-149.72g·L-1,(MLVSSMLSS),0.360.50;,MLVSS(r=0.918,p=0.003),SO42-30%～95%.,,,、、;,,、.、、、N2;.:UASB;;;N2:X703.1　:A　:0250-3301(2009)12-3630-09:2009-01-14;:2009-03-16:(863)(2006AA06Z322):(1985～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]teristicinStart-upProcessofUASBTreatmentforHighlySulfate-containingWastewaterYANGLi-ping1,2,ZHENGXiao-hong2,LUOHui-dong2,ZENGGuo-qu2,3,XUMei-ying2,SUNGuo-ping2(1.CollegeofBioscience&Bioengineering,JiangxiAgriculturalUniversity,Nanchang330045,China;2.GuangdongProvincialKeyLaboratoryofMicrobialCultureCollectionandApplication,GuangdongInstituteofMicrobiology,Guangzhou510070,China;3.SchoolofEnvironmentalScienceandEngineering,SunYat-SenUniversity,Guangzhou510275,China)Abstract:Changesofsludgecharactersinasulfate-reducingUASBreactorduringthe243dayofdomesticationprocessweretrackingcomparison.Resultsshowedtheaveragediameterofgranularsludgeininitialdomesticationstagesdecreasedto0.99mmcomparedwith1.82mmofincubatingsludge,butwhichwasfoundtobeincreasingalongwiththeenhanceoftheloadingrateofreactor,theshortenofHRT,andtheaccelerationofhydraulicup-flowvelocity.N2strippingwasusedtoremoveH2Sproducedinthereactor,andtheaveragediameterofgranularsludgeincreasedto1.51mmquicklywhenN2strippingwiththefluxof60mL·min-1wasused,butitdecreasedwhenthefluxofN2improvedto100mL·min-1.Differencesinaveragediameteramonggranularsludgesindifferentheightofthereactorbecameunconspicuousthroughcontinuousstripping.ChangesintheMLVSSandtheratioof(MLVSSMLSS)displayedsimilartrends,forwhichfirstlydecreasedintheearlierperiodthenincreasedto33.59g·L-1andwentupto49.72g·L-1steadilyinthelaterstage,andthenraisedfrom0.36to0.50subsequently.Sulfatereductionefficiencyimprovedfrom30%to95%,whichwasstronglycorrelatedwiththeMLVSS(r=0.918,p=0.003).Scanningelectronmicroscopeanalysisshowedinoculatingsludgewascoarseandincompact,andwiththedominantspeciesoffilamentousbacteria,bacillus,cocci,butgranularsludgewithdensemicroorganismcommunitystructurewereobserved,andinwhichvibrioandbacilluswerethedominantspecies.Keywords:UASBreactor;sulfate;sludgecharacteristics;N2stripping　　(up-flowanaerobicsludgebed,UASB),[1].、、、、、SO2-4,.(sulphatereducingbacteria,SRB)SO2-4H2S,SRB.SO2-4SRBSO2-4、3012200912　　　　　　ENVIRONMENTALSCIENCEVol.30,No.12Dec.,2009DOI:10.13227/j.hjkx.2009.12.027[2,3].UASB[4,5],UASB,,UASB.()[6].,,0.6mm[7].,、、[8].2[9,10].,、、pH、[11～15].,UASB.UASB、、、N2,,UASB.1　1.1　UASB(1)15cm,110cm,19.4L.1　Fig.1　Schematicdiagramofthereactor1.2　　　,2∶1,(MLSS)67.67g·L-1,(MLVSS),23.36g·L-1.1.3　,1.1.4　0.51～1.8kg·(m3·d)-1,COD1.5～5.0kg·(m3·d)-1,1,pH7.(5.5～31℃),pH6.4～6.8.、COD、(),HRT80h,150d60h,180d48h,210d24h.、、3.1　Table1　Componentofsynthesiswastewatermg·L-1mg·L-15000KH2PO4250200NH4Cl100Na2SO4(SO2-4)1200～1700NaHCO32000MgSO430(NH4)2CO3100CaCl2101.5　120～140d,78%,141dH2S,N20.06L·min-1,2,2h.H2S,,,.N290%.0.68kg·(m3·d)-1,,N20.06L·min-1,2,4h.0.9kg·(m3·d)-1,0.1L·min-1,2,6h.1.8kg·(m3·d)-1UASB0.1L·min-124h,1mol·L-1NaOH,.1.6　363112:UASB,..COD(GBT11914-89),SO2-4(GBT11899-89),(HJT60-2000);MLSSMLVSS;:10mL,100mL,10mL,.Qwin,.:1L,,.,t,V=Ht(V,H,t).,20～30,.Excel.FISH[16].2　2.1　UASB2.243d,1.8kg·(m3·d)-1,95%,COD5.0kg·(m3·d)-1,COD35%..2　Table2　OperatingparametersofUASBdL·h-1hSO2-4kg·(m3·d)-1CODkg·(m3·d)-1SO2-4%COD%0～300.2425800.511.530～3513±231～1200.2425800.511.535～7813～20121～1500.2425800.511.578～9020～25151～1800.323600.68290～9525～35181～2100.404480.92.595±235±2211～2430.808241.85.095±235±22.2　MLVSSMLSS3MLVSSMLSS2、3.30d(MLVSS)(MLVSSMLSS),,.2　MLVSSFig.2　MLVSSvariationofsludgeindomesticatedprocess3　MLVSSMLSSFig.3　RatioofMLVSSMLSSvariationindomesticatedprocess　　　4、5MLVSSMLVSSMLSS.MLVSS、MLVSSMLSS,MLVSS、MLVSSMLSS0.920.78.MLVSSMLVSSMLSS0.91.3632　　　　　　304　MLVSSFig.4　RelationofsulfateeliminationrateandMLVSS　5　MLVSSMLSSFig.5　RelationofsulfateeliminationrateandMLVSSMLSS　2.3　deltaProteobacteriaSRB(Clostridiumspp.)、Desulfovibriospp.Geobacter、Desulfomonas、Desulfuromonas、Desulf-Omicrobium、Bilophila、PelobacterMethanobacteriaceae(3),6.,3　FISHTable3　Alistof16SrDNA-targetedoligonucleotideprobesusedinFISHstudy(5′-3′)SRB385deltaProteobacteriaSRB(Clostridiumspp.)CGGCGTCGCTGCGTCAGG[17,18]SRB687Desulfovibriospp.、Geobacter、Desulfomonas、Desulfuromonas、Desulfomicrobium、Bilophila、PelobacterTACGGATTTCACTCCT[19]MB1174MethanobacteriaceaeTACCGTCGTCCACTCCTTCCTC[20]6　FISHFig.6　BacterialabundancevariationofsludgedetectedbyFISH　Desulfovibriospp.、Geobacter、Desulfomonas、Desulfuromonas、Desulfomicrobium、Bilophila、PelobacterSRB6876%±0.5%30%±0.5%.SRB385,10%～20%.MB1174,2%～6%.2.4　(78).7,(7A),,,(7B).(7C、7D),,.(7E、7F),、、、,.,(8A),(8B),(8C、8D),,、.(8E、8F),.2.5　0、30、120、150、180、210240d37,9.74,0.014、0.018、0.0230.046m·h-1.,、363312:UASB7　Fig.7　Scanningelectronmicroscopechartoftheinoculatinggranularsludge　8　Fig.8　Scanningelectronmicroscopechartoftheanaerobicgranularsludgeintheendofdomesticated　、N2,.30d120d,0.6～1.0mm,49.9%59.7%,1.0～2.0mm,3634　　　　　　3043.7%39.5%;141d,150d0.6～1.0mm1.0～2