胞外聚合物EPS构成的影响因素分析

33EnvironmentalScience&Technology3312F201012Vol.33No.12FDec.2010EPS100044EPS。EPS。EPS、、EPS。EPS。EPSX131.2Adoi10.3969/j.issn.1003-6504.2010.12F.1111003-6504(2010)12F-0420-05AnalysisofInfluencingFactorontheComponentofExtracellularPolymericSubstance(EPS)CAOXiu-qin,ZHAOZhi-lingBeijingUniversityofCivitEngineeringandArchitectuee,Beijing100044,ChinaAbstractExtracellularpolymericsubstance(EPS)inbiologicalwastewatertreatmentsystem,incarnatingthefunctionandcompositionofmicroorganisms,wouldcontributeobviouslytosludgecharacteristicsandtheperformanceofthesystem.However,standardmethodformeasuringEPShasbeennotdeterminedbecauseofitsnumerousinfluencingfactors.Therefore,thefactorofEPSwasanalyzedsyntheticallyinthispaper,suchastheinfluentcomponentsandoperatingconditions,whichincludingsludgeloading,dissolvedoxygenandhydraulicdetentiontime.Differentextractingmodeandmeasuringmethodwerealsocomparativelyanalyzed.ItwouldacceleratethecontroleffectonthetreatmentefficiencyofbiologicaltreatmentsystembyfurtherstudyonEPSanditsfunctionKeywordsextracellularpolymericsubstance(EPS);influentcomponents;operatingconditions;extractingmode2010-09-062010－10－25MOC06-k4-28BOP-06(1965-)()010-68322128()[e-mail]。EPS(Extracellularpolymericsub－stances)[1][2-4]。EPS。。[2,5-7]EPS70%~80%20%~30%、。EPS、EPS。,(Frolundetal,1996;Liuetal.,2002;,20042008Nietal.,2009)。EPS10000,、。EPS、,Folin、。EPSEPS12F。1EPSEPSEPS。Sponza[8-9]EPS70~71mg/gMLVSS6.2~6.5mg/gMLVSS、、324~48mg/gMLVSS11~12mg/gMLVSS。[10]EPSEPS。EPS。EPS。[11](、)EPS、。、EPS、。2EPS2.1EPSEPS[12]。EPSEPS。EPS。[13]EPS0.3kgBOD5/(kgMLSS·d)3kgBOD5/(kgMLSS·d)EPS23.4mg/gVSS14.5mg/gVSS11.6mg/gVSS6.92mg/gVSS。[14-15]EPSEPS。2.2DOEPS。Sponza[9]DO0.5～2mg/L,、DODO0.1mg/L,。DO04.2mg/LEPS、。DOEPS。DO0EPSEPS。Shin[16],DO,DO、EPS。DOEPS。[17]EPSDO,、EPS,2～3DO4.5mg/LDO4.5mg/LEPSDO。DOEPSDOEPSEPSDODO4.5mg/LDOEPS。2.3Wilèn[18]EPS。[19]、、3EPS-20℃EPS4℃。EPS-20℃EPS9%、20%20%EPS3(85%,80%4℃80%70%-20℃30%60%)。[13]EPS10℃EPSEPS42133DNAEPS10℃15℃EPSDNA15℃30℃EPS14%DNA。EPS。2.4pH[20]EPSpH。(pH=3)EPSpH=750%30%65%~70%(pH=11)EPSpH=720%~30%15%20%~50%。[13]pHEPSpH=5~6EPSpH=7pH=8EPSpH=9EPS。pHEPS。[17]pH7EPSpH5.58.5EPSEPS。EPS,pH。2.5SRTLiao[21]SBRSRT4～20dEPSEPSSRTGulas[22]1979。LiaoSRT4~9d16~20d。SRTEPS。2.6EPS、[23]。。[24]Mn2+、Mo6+Zn2+lmg/LMn2+、Mo6+Zn2+EPS(0.05g/L)Mn2+EPS、Zn2+EPSMo6+EPS。[13]Ca2+01mmol/LEPS。EPS60%DNA10%7%6%。[25]MnPbCd。。3EPS3.1EPSEPSEPS。EPS。(Murthyetal,[26])、(Jorandetal,[27])(Morgan,etal,[28])、、、EDTA(CER)EPS。LiuFang[29]20000r/30minEPS7.3mgEPS6.5mg0.8mg。80℃EPS8(57.9mg)、18、78.63.5。[30]5-NaOHEPS232.0、159.7mg/g7.6～9.4mg/gEPSEPS50%～80%DNA。[31]4(、、、)EPS42212F(80℃、30min)(320W、40s)、30.3、8.1mg/gVSS24.3、10.8mg/gVSS。EPS3.72.3。EPSEPS。。[32]EPS35%DNA。Liu[29]-、-、EDTA、CER5-EPS。-EDTA、CER-。3.2EPSEPS、、DNAEPSEPS。EPSEPS。、。3.2.1[33]-。Frolund[1]-EPS。Benetti[34]－50g、、。-108%82%-69%61%。-。3.2.2Folin-Lowry。Frolund[1]、Folin-Lowry、Folin-LowryEPSNN6.25Folin-LowryNFolin-LowryEPS。Folin-LowryFolin-LowryFolin-LowryFolin-LowryFolin-Lowry。EPS。[35]-,,Folin,。Ni[36]EPS。EPS。EPS61%、21%18%、EPS。4EPSEPS。EPSEPSEPS。EPSEPSEPS、。[][1]FrolundB,PalmgrenR,KeidingKetal.Extractionofex－tracellularpolymersfromactivatedsludgeusingacationex－changresin[J].WatSciTech,1996,30(8):1749-1758.[2]UrbainV,BlockJC,ManemJ.Bioflocculationinactivatedsludge:ananalyticapproach[J].WatRes,1993,27(5):829-838.[3]DignacMF,UrbainV,RybackiD,etal.Chemicaldescrip－tionofextracellularpolymersimplicationonactivatedEPS42333sludgeflocstructure[J].WatSciTech,1998,38(8):45-53.[4]NwyenysE,BaeyensJ,DewilR,etal.Advancedsludgetreatmentaffectsextracellularpolymericsubstancestoim－proveactivatedsludgedewatering[J].HazardMat,2004,106(B):83-92.[5]KeidingK,NielsenPH.Desorptionoforganicmacro－moleculesfromactivatedsludgeeffectofioniccomposition[J].WatRes,1997,31(7):1665-1672.[6]ForsterCF,ClarkeAR.Theproductionofpolymerfromactivatedsludgebyethanolicextractionanditsrelationtotreatmentplantoperation[J].WaterPollut,Control,1983,82:430-433.[7]LiuH,FangHHP.ExtractionofextracellularpolymericsubstancesEPSofsludge[J].JournalofBiotechnology,2002,95:249-256.[8]SponzaD.T.Extracellularpolymersubstancesandphysico-chemicalpropertiesofflocsinsteadyandunsteady-stateactivatedsludgesystems[J].ProcessBiochemistry,2002,37:983-998.[9]SponzaD.T.Investigationofextracellularpolymersub-stances(EPS)andphysicochemicalpropertiesofdifferentactivatedsludgeflocsundersteady-stateconditions[J].En－zymeandMicrobialTechnology,2003,32:375-385.[10],,,.[J].,2007,30(2):8-12.GeLi-yun,WangHong-wu,MaLu-ming,etal.Influenceofextra-cellularpolymericsubstancesinaerobicactivatedsludge[J].EnvironmentalScience&Technology,2007,30(2):8-12.(inChinese)[11].(EPS)[D].:,2006.WuZhi-gao.StudyontheEffectofExtracellularPolymerSubstancesontheSettleabilityoftheSludgeandItsRoleinBiologicalRemovalofPhosphorus[D].Changqing:ChongqingUniversity,2006.(inChinese)[12]SheintuchM.Steady-statemodellingofreactor-settlerin－teraction[J].WatRes,1987,21:1463-1472.(inChinese)[13],,.EPS[J].,2004,24(4):614-620.ZhouJian,LongTeng-rui,MiaoLi-li.Effectofextracellu－larpolymericsubstances(EPS)onsedimentationofactivat－edsludge[J].ActaScientiaeCircumstan