不同碳源对固定化反硝化菌脱氮的影响谭佑铭

32　22003　3　　　JOURNALOFHYGIENERESEARCHVol.32　No.2Mar.　2003　95:1000-8020(2003)02-0095-03··谭佑铭　罗启芳,　430030:为寻找较为经济、高效的有机碳源,分别外加葡萄糖、蔗糖、甲醇和乙酸,以研究它们对固定化反硝化菌脱氮的影响。结果表明,固定化反硝化菌能有效利用上述碳源,进行反硝化作用。其中采用葡萄糖、蔗糖和乙酸的反硝化速率较快。HRT为6h时,脱氮率达96%以上。若碳源较丰富,反硝化过程中的氨化作用并不明显,被转化成氨氮的硝态氮低于被还原总量的5%。采用固定化反硝化菌处理CODNO-3-N较低,且不外加碳源,起始浓度分别为45.2和24.6mgL的实际水样,分别经过60h和32h后,脱氮率达90%以上。固定化细菌能利用水中部分的有机物进行反硝化作用。:反硝化　碳源　固定化细菌:R123.6　　　　　　:AStudyondenitrificationusingdifferentcarbonsourcesTanYouming,LuoQifangInstituteofEnvironmentalMedicine,TongjiMedicalCollege,HuazhongUniversityofScience&Technology,Wuhan　430030,ChinaAbstract:Inthisstudy,benchscaletestswereconductedtostudythepotentialsofimmobilizeddenitrifiertoreducenitrateinthepresenceof4differentcarbonsources:glucose,canesugar,methanolandaceticacid.Theresultsshowedthatthecarbonsourcescanbeusedbytheimmobilizedbacteriaasexogenouscarbonsources.Whileusingmethanol,theaveragedenitrifyingvelocitywaslowerthantheothers.Dissimilatoryreductiontoammoniumwasnotsignificantandac-countedforlessthan5%ofreducednitrate.Bya6-hourhydraulicresidencetime,thedenitrificationrateswerehigherthan96%.ThenitrateenrichedwaterofSlakewasalsotreatedbytheimmobilizeddenitrifiertostudythecharacterofdenitrification,especiallyontheusingofnaturalcarbonsourcesaselectrondonotor.Theresultsshowedthatmorethan90%ofthenitrateinthewatercouldbereducedbytheimmobilizedbacteria,andmorethan20%ofthenaturalcarbonsourcesinthewatercouldbeusedbytheimmobilizedcells.Keywords:denitrification,carbonsources,immobilizedbacteria:(No.39870664):,,,　　,,[1]。,,,。,()。、[2～4],、,。、,,、、,。,,,。1　1.1　1.1.1　　PIX-Ⅱ()。pHS-2C();722();FA104()。1.1.2　　K2Cr2O7,KNO3、、、。1.2　[1]。1.3　,[6],、、,(non-growthmedium)。S,KNO3,NO-3-N50mg25mg,。。　Table　Conditionsofsyntheticwater(ml)COD(mgL)NO-3N(mgL)CODNO-3-NCNpHDO350130157.322.78.57.22.9350131458.122.68.87.23.5350129054.223.88.17.23.4350126256.122.58.77.23.6SA35051.945.21.15—6.83.1SB35051.924.62.11—6.83.11.4　,500ml。55g,25g。,,、,、,。(30±1)℃,,(DO)、pH、NO-3-N、NO-2-N、NH+4-N、CODCr,[7]。2　2.1　1,NO-3-N。1　NO-3-NFig.1　NO-3-Nvs.timeduringdenitrificationusingdifferentcarbonsource6h,97.2%、96.7%、86.5%98.5%。,1.5h25.3mg(L·h);1.5～3.0h,9.3mg(L·h);3.0～6.0h,1.2mg(L·h)。,4.5h,8.6mg(L·h)。1.5h,0.078mg/(L·h)。,、,。,、,;,,,;———[8],,,。,:CH3OH※HCOH※HCOOH※CO2,,,,pH[9],。,,,、。2.2　,NO-3-N。,[6]。CODNO-3-N;CODNO-3-N,,。,NO-3-N5%。,,,。2.3　DODO2.9～3.6,。PVA,200μm[10]。,PVADO,DO,。NO-2,。,NO-2-N,0.5mgL,NO-2-NN2。DO,,[11],。2.4　,,SA、B60h,NO-3-N2.712.09mgL,96%92%。2。2　NO-3-NFig.2　NO-3-Nvs.timeintrialexperiment、,。、[12]。,96　　　　　　32:(No.299065)(No.399706280):,,,。S,COD51.9mgL。60h,COD38.7mgL(A)40.8mgL(B),COD25.4%21.6%。,,,NO-3-N,,COD,。,,,。,,CODN,,N2O[13],,。3　1　,..,2001,18(6):371—3732　,..,2002,31(1):19—213　LouisAS,MajaVV.Fiveyearsofnitrateremoval,denitrificationandcarbondynamicsinadenitrificationwall.WatRes,2001,35(14):3473—34774　SaraH,MikaelP.Metabolicpropertiesofdenitrifyingbacteriaadaptingtomethanolandethanolinactivatedsludge.WatRes,1998,32(1):13—185　WuKY,KeithDW.CellimmobilizationusingPVAcrosslinkedwithbo-ricacid.BiotechnolBioeng,1992,39447—4496　AlunnaJC,BizeauC,MolettaR.Nitrateandnitritereductionswithanaerobicsludgeusingvariouscarbonsources:glucose,glycerol,aceticacid,lacticacidandmethanol.WatRes,1993,27(8):1303—13127　..3.:,1989,246—3568　..,1994,13(10):8—129　GorgeRN.Denitrificationwithmethanol:microbiologyandbiochemistry.WatRes,1980,14(2)531—53710　UemotoH,SaikiH.Behaviorofimmobilizednitrosomonaseuropaeaandparacoccusdenitrificansintubulargelfornitrogenremovalinwastewater.Prog.Biotechnol,1996,11:486—49311　BaumannB,SnozziM,MeerJR,etal.Developmentofstabledenitrify-ingculturesduringrepeatedaerobic-anaerobictransientperiods.WatRes,1997,31(8):1947—195412　KoelmansAA,HeijdeAV,KnijffLM.Integratedmodelingofeutrophica-tionandorganiccontaminantfate&effectinaquaticecosystems.WatRes,2001,35(15):3517—353613　HirokiI,KeisukeH,TomonoriM.Nitrousoxideproductioninhigh-load-ingbiologicalnitrogenremovalprocessunderlowCODNrationcondition.WatRes,2001,35(3):657—664(2002-06-19)32　22003　3　　　JOURNALOFHYGIENERESEARCHVol.32　No.2Mar.　2003　97:1000-8020(2003)02-0097-04··施玮　蒋颂辉　朱惠刚,　200032:为制定饮用水源中藻类限值,结合国内外文献调查研究毒理学试验和现场生态学调查资料,根据世界卫生组织(WHO)和卫生部推荐的饮用水中微囊藻毒素MC-LR的限值、藻类生物量和MC-LR浓度关系以及毒理学实验所得到的藻类的无作用浓度而制定饮用水源中藻类限值。结果显示,推荐饮用水源中藻类三级限值分别为:安全限值:1.0×104个升;警戒限值:2.1×105个升;危险限值:1.2×106个升。提示饮用水源中藻类限值在日常工作中有显著意义和可行性。:藻类　微囊藻毒素　饮用水　水源　限值:R123.1　　　　　　:AStudyonguidelineofalgainsourcewaterShiWei,JiangSonghui,ZhuHuigangDepartmentofEnvironmentalHealth,SchoolofPublicHealth,FudanUniversity,Shanghai　200032,ChinaAbstract:Toxicbloominthesourcesofdrinkablewaterhasbeenapublichealthproblem.Hinderedbythecompli-catedanalysisprocedure,verylimitedeffortshasbeendoneininvestigatingtoxinlevel.PatronizedbytheChineseAcademyofPreventiveMedicine,astudyonalgahygienecriteriaofdrinkablewatersourcesinChinawasconducted.Availableliter-aturewasreviewedandecologicalresearchandtoxicologicaltestswerecarriedout.Thecriteriaonalgabiomassindrink-