超声波强化微生物对偶氮染料AO7的生物降解机理研究

452厦门大学学报(自然科学版)Vol.45No.220063JournalofXiamenUniversity(NaturalScience)Mar.2006AO7:20050120:(1980-),,.*:[e-mail]沈政赢,袁东星*,马剑,李猛((),,361005):SBR,AO7.:,6.4W/L;,2000mg/L;,0.4mL/min;,5min;,25kHz.AO7.,..AO7.,,,AO7.:;;AO7;:X703:A:04380479(2006)02024305,7105t.10%.,,,.,,,,.,.,.,..,pHH2O2Fenten,..,.[1~5].,,..Schlfer[1]COD.,[6].AO7,.AO7,AO7.AO7100[7],AO7,.11.11L(8cm,35cm)SBR.0.6L,8h.ACO,,,6~8mg/L.,1..1.2,1L0.2gAO7,,NH4Cl,0.1g;NaH2PO42H2O,0.15g;CaCO3,0.060g;MgCl21SBR1-2-Fig.1SchematicdiagramoftheUSassistedSBRsystem4H2O,0.011g..pH7.8.,.1.3AO7UVVIS,(DR/2010HachCo.,USA)483nm;(ABS)12(HPLC)(510,WatersCo.,USA,SPD10AUVVIS,SimutchzCo.,Japan).Nucleosil-C18,5m,4.6mm250mm,V()V()=12,0.6mL/min,234nm.pH,COD(MLSS)[8].Brodford[9].1.4..,(MLSS)3000mg/L.,1,.AO7,.(1).[A]0AO7,[A]ttAO7.1(L20544)Tab.1TheorthogonalarraytablefortheUSoptimizationexperimentsABCDE11.615000.1226.42050050.2314.4251000100.3425.6302000250.4540.0A:(W);B:(kHz);C:(mg/L);D:(min);E:(mL/min).=[A]0-[A]tt(mg/Lmin)(1)22.1,.2.R,R16.6R16.3,,AO7.AO7:.2k11k51,6.4W/L,AO7.,.2k13k43,,AO7.[6]2Tab.2TheresultoftheorthogonalexperimentsABCDE/%111111-5.021112210.5312211-6.341222210.75233139.662343423.772434410.882442332.293141215.410332218.3113433411.012321434.1134123310.21443342315444415.516421347.417513215.3185344420.9195421202052133-3.4k1j2.57.33.22.81.6k2j19.12.94.613.47.9k3j9.712.18.010.212.4k4j6.611.919.58.913.3k5j5.7R16.69.216.310.611.7244()2006t/min2AO7!;∀;#(A2B3C4D2E4)Fig.2AO7concentrationasfunctionofreactiontime.,AO7.2,AO7.,A2B3C4D2E4,6.4W/L,25kHz,2000mg/L,5min,0.4mL/min,AO7.2.2AO7,AO7.2AO7.2,,AO7.AO71.736mg/Lmin2.646mg/Lmin,AO750%.2,,AO7.,AO7.AO7COD,COD.3,1,.,.Schlfer[1].t/min3!;#Fig.3Relationshipofln([A]t/[A]0)andreactiontimeforthedegradationofglucose4AO7Fig.4ThechemicalstructuresofAO7anditsdegradationproductsk,k0.071d-1,k0.089d-1.,.2.3AO7.,.AO7(ABS)12.AO74.Gottlieb[7]AO7AO7100.AO7.Nrtemann[10]12,,.HPLC12,12.,12.AO7ABSAO7HPLC,2452:AO73AO7Tab.3DLRandProteinaseactivitywithdifferentUSintensity/WL-1/mgL-1min-1/%/%1.61.8441.63113.10.4330.39011.014.41.9681.65319.00.4520.38118.6401.6861.5439.30.5190.39039.05ABS.,ABS,.,ABS,ABS.ABS.,ABS.ABS.5,140min,ABS40mg/L,2,AO7.,.t/min5ABS!;#(A2B3C4D2E4)Fig.5ABSconcentrationasthefunctionofreactiontime2.4,.,[11].,A,AO7,3.,1.6,14.4,40W/L,,11.0%,18.6%,39.0%,,AO713.1%,19.0%,9.3%.,.3,1.6W/L,13.1%,11%.40W/L,39.0%,9.3%.,.,[5],,,,.,,.,AO7.3AO7.AO7..:[1]SchlferO,OnyecheT,BormannH,etal.Ultrasoundstimulationofmicroorganismsforenhancedbiodegradation[J].Ultrasonics,2002,(40):25-29.[2]SakakibaraM,WangD,TakahashiR,etal.Influenceofultrasoundirradiationonhydrolysisofsucrosecatalyzedbyinvertase[J].EnzymeandMicrobialTechnology,1996(18):444-448.[3]WoodBE,AldrichHC,IngramLO.Ultrasoundstimulatesethanolproductionduringthesimultaneoussaccharificationandfermentationofmixedwasteofficepaper[J].BiotechnologyProgress,1997(13):232-237.246()2006[4]MechnsnerK,WuhrmannK.Cellpermeabilityasaratelimitingfactorinthemicrobialreductionofsulfonatedazodyes[J]Eur.J.Appl.Microb.Biotech.,1982(15):123-126.[5]DengCX,SielingF,PanH,etal.Ultrasoundinducedcellmembraneporosity[J].UltrasoundinMed.&Biol.,2004(30):519-526.[6]MohammadHE.Acombinationofultrasoundandoxidativeenzyme:sonobiodegradationofsubstitutedphenols[J].UltrasonicsSonochemistry,2003(10):241-246.[7]GottliebA,ShawC,SmithA,etal.Thetoxicityoftextilereactiveazodyesafterhydrolysisanddecolorization[J].JournalofBiotechnology,2003(101):49-53.[8],∃%.[M].4.:,2002.[9]DryerRL,LataFG.ExperimentalBiochemistry[M].NewYork:OxfordUniversityPress,1989.[10]NrtemannB,BaumgartenJ,RastHG,etal.Bacterialcommunitiesdegradingaminoanddihydroxynaphthalene2sulfonates[J].Appl.Environ.Microbiol.,1986(52):1195-1202.[11],,,.[J].:,1999(38):537-539.ImprovementofAcidOrange7BiodegradationbyUltrasoundAssistedBioreactorsSHENZhengying,YUANDongxing*,MAJian,LIMeng(StateKeyLaboratoryofMarineEnvironmentalScience(XiamenUniv.),EnvironmentalScienceResearchCenter,XiamenUniv.,Xiamen361005,China)Abstract:Acombinationtechniqueofultrasound(US)exposureandaerobicactivatedbiomassinaSequencingBatchReactor(SBR)hasbeendeveloped.ThestudywascarriedouttoinvestigatetheeffectofUSonthebiologicalcolorremovaloftextileeffluentscontainingazodyeAcidOrange7(AO7).Theoptimumparameterswerefoundtobe:USintensity,6.4W/L;glucoseinflux,2000mg/L;aerateflux,0.4mL/min;USexposureinterval,5min;USfrequency,25kHz.USintensityandglucoseinfluxwerethepredominatingfactors.ThebiodecolorizationrateincreasedasUSintensityincreased,atacertainlevelitdecreasedifUSintensitycontinuallyincreased.Thedecolorizationratehadbeenimprovedwiththeincreasingofglucoseinflux.Basedontheexperimentdata,itwasfoundthattheUSexposurenotonlysignificantlyincreasedAO7biodecolorizationratebutthebiodegradationefficiencyofAO7degradationproduct.ThecellmembranepermeabilitywasimprovedbyUSexposure.Howevernolinearrelationshipbetweentheimprovementofpermeabilityandthebiodecolorizationratewasfound.Keywords:ultrasoundexposure;improveddecolorizationrate;azodyeacidorange7;activatedbiomass2472:AO7