城市生活污水SNAD生物膜脱氮特性郑照明

2017,37(4)1322~1330ChinaEnvironmentalScienceSNAD,*,,,,,(,,100124)(SNAD).SNAD.NH4+NNO2N(TIN)0.121,0.180,0.267kgN/(kgVSS·d);0.211,0.053kgNO2N/(kgVSS·d).SNADpH5~9,pH.SNADNO2NFNA.NO2N100,150mg/L,FNA70,100μg/L,NH4+N0.087,0.029kgN/(kgVSS·d).,SNAD.SNAD,.SNADpHX703.5A10006923(2017)04132209NitrogenremovalperformanceofSNADbiofilmculturedbydomesticwastewater.ZHENGZhao-ming,LIJun*,HOUAi-yue,MAJing,DUJia,ZHAOBai-hang,YANGJing-yue(NationalEngineeringLaboratoryforAdvancedMunicipalWastewaterTreatmentandReuseTechnology,EngineeringResearchCenterofBeijing,BeijingUniversityofTechnology,Beijing100124,China).ChinaEnvironmentalScience,2017,37(4)1322~1330AbstractThenitrogenremovalperformanceofsimultaneouspartialnitrification,anaerobicammoniumoxidizationanddenitrification(SNAD)biofilmwasinvestigatedinbatchtests.TheSNADbiofilmperformedgoodanammoxanddenitrificationactivities.TheNH4+N,NO2Nandtotalinorganicnitrogen(TIN)removalratesofanammoxactivitywere0.121,0.180and0.267kgN/(kgVSS·d),respectively.Thedenitrificationandnitriteoxidationactivitieswere0.211and0.053kgNO2N/(kgVSS·d),respectively.ThesuitablepHrangesforanammoxactivitywere5.0to9.0.ThetheinhibitioneffectofpHontheanammoxbacteriacouldbelargelyrelievedbybiofilm.Besides,theSNADbiofilmwasfoundtoperformhightolerancetotheinhibitionofNO2Nandfreenitrousacid(FNA).TheNH4+Nremovalratesforanammoxprocesswere0.087and0.029kgN/(kgVSS·d)withtheNO2Nconcentrationsof100mg/L(FNAconcentrationof70μg/L)and150mg/L(FNAconcentrationof100μg/L),respectively.TheSEMobservationsindicatedthatthebacteriaintheouterpartoftheSNADbiofilmweremainlyshortrod-shaped.IntheinnerpartoftheSNADbiofilm,thebacteriaweremainlycrater-shaped,whichshouldbeanammoxbacteria.KeywordsSNADbiofilmnitrogenremovalperformanceanammoxnitriteeffectofpH.,[1].SNAD(simultaneouspartialnitrification,anaerobicammoniumoxidizationanddenitrification),,[2].SNAD,SNAD[3].,20160801(2014ZX07201011);(51308010);(20131103120017);(2015ZZ10)*,,[e-mail]4SNAD1323(NOB,nitriteoxidizingbacteria),.[4]SNAD,(0.15~1.4mg/L),(AOB,ammoniaoxidizingbacteria).Ge[5],(10min/20min)NOB.SNAD.pH[69]..Strous[6]98mg/L.DapenaMora[7]Lotti[8]350400mg/L,50%.[1011].,,.SNAD,NO2NpH,SNAD.11.1SNADSNAD1.,89.5L(2.07).SBR(sequencingbatchreactor),,(K3,AnoxKaldnes,),25mm,,4mm,34L,77.7L,81%.2.,,(20cm),20cm,20mm.,500L/h,30℃.116795432181SBRFig.1TheschematicdiagramofSBRreactor1;2;3;4;5SBR;6;7DO;8pH;9;10;11PLC2SNADFig.2ThephotoofKaldnesringinSNADbiofilmreactor1.2SNAD,:CODCr200~300mg/L;NH4+N60~80mg/L;NO2N1mg/L;NO3N1mg/L;TOC50~60mg/L;TN100~140mg/L;pH7.5~8.0;300~400mg/L.:(5min),(20min/20min),(20min),(10min),(10min),(1min).1324376.,,,,.5.6,0mg/L.SNAD60d,.NH4+N,NO2N,NO3NCOD2,2,7,50mg/L,COD71%,TIN80%~90%.1.31.3.1SNAD.30.1.3.2,NH4Cl,NaNO2..Tang[12].HClNaOHpH.NO2N,pH7.0,NH4+N70mg/L,NO2N70,100,150,200,250,300mg/L.1.1(mg/L)Table1Thesyntheticwastewaterofbatchtests(mg/L)NH4+N7000NO2N707070NO3N000048001.3.31000mL,50,3.[12]:(1);(2),500r/min,,10min(99.999%);(3),30℃.:(1);(2),250mL/min(DO6mg/L),,500r/min,30℃..(1).=2460()(1):mg/L;min;g.:,NH4+NNO2N10mg/L,NH4+NNO2N10mg/L;,NH4+NNO2N10mg/L,.:kgN/(kgVSS·d).1.3.4,,,(VCX105PB),90%,30min.,,,,.1.4NH4+N:;NO2N:N(1);NO3N:;NH4+N,NO2NNO3NTIN;DOpH:WTW/Multi3420;:HitachiS4300;CODCr:;(SEM):.,PBS2~3,2.5%1.5h,PBS3,50%,70%,80%,90%100%,10~15min,,37℃.,1500nm,HitachiS−4300.4SNAD1325FNA[13].22300pH273(NON)46FNA14e10TC(2):T().22.1SNAD2.1.1SNADSNAD3..NH4+NNO2N70mg/L,,NH4+NNO2N,NO3N.NO2N/NH4+N=1.45,NO3N/NH4+N=0.29,Strous[6].NH4+NNO2NTIN0.121,0.1800.267kgN/(kgVSS·d).SNAD4.0.211kgNO2N/(kgVSS·d).0204060801001201400306090120150180210240270NH4+-N(min)NO2--NNO3--N(mg/L)TIN3SNADFig.3TheanammoxactivityofSNADbiofilmStrous[14]0.5%,..5.60mg/L.SNAD.SNAD,[15],;,AOB,[16];AOB[17];,SNAD,,[18].02040608010012014016018020022024001020304050607080NO2--N(mg/L)(min)4SNADFig.4ThedenitrificationactivityofSNADbiofilm2.1.2SNADSNAD5.SNAD,0.053kgNO2N/(kgVSS·d).SNAD(20min/20min),5.60mg/L.Jardin[19]1.0mg/L,818min,SNADNOB.Gilbert[20]NOB15~20min.SNADAOB,NOB[15,17].Morales[15]SNAD,4mg/L8mg/L132637,150m250m,0mg/L.,NOB.VanderStar[21]NO2NNOB.,SNADNOB,NOB.Gilbert[20],NOB,NOB.SNAD,NOB,NOB.02040608010012014016018020022024026028030001020304050607080NO2--N(mg/L)(min)5SNADFig.5ThenitriteoxidationactivityofSNADbiofilm2.2NO2NNH4+NNO2NNH4+N6.NH4+N7.NO2N,NH4+N.NH4+N0.0990.009kgN/(kgVSS·d).NO2N100150mg/L,NH4+N0.0870.029kgN/(kgVSS·d),NH4+N87.4%29.6%./(%)FNA501001502002503003500204060801001201400.000.050.100.150.000.050.100.150.200.250.3019.9%8.8%13%29.6%87.4%/(%)100%NO2--N(mg/L)NH4+-N[kgN/(kgVSSd)]FNA(mg/L)6NO2NNH4+NFig.6EffectofNO2NconcentrationsontheNH4+Nremovalrateofanammoxprocess02040608010012014016018020022024026028030001020304050607080200mg/L(min)100mg/L150mg/LNH4+-N(mg/L)250mg/L300mg/L70mg/L7NO2NNH4+NFig.7EvolutionofNH4+NconcentrationsofanammoxprocessunderdifferentNO2NconcentrationsStrous[6],NO2N98mg/L.Egli[9]NO2N180mg/L,3d.Tang[10],NO2N360mg/L,UASB,NO2N700mg/L,,.Kimura[11],NO2N430mg/L,37%;NO2N750mg/L,.4SNAD1327NO2N[69].SNAD,NO2N,NO2N.NO2NTang[10]Kimura[11].SNAD,NO2N10mg/L,NO2N.SNAD,,1~2mm,,SNAD.,,[22].Tan[23]