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第31卷第10期 2008年10月合肥工业大学学报(自然科学版)JOURNALOFHEFEIUNIVERSITYOFTECHNOLOGYVol.31No.10 Oct.2008 :2007-11-12;:2008-02-25:(40672154);(NCET-06-0541)(07010302165): (1968-),,,;(1968-),,,,,.DB1刘 咏1, 钱家忠2, 李云霞2, 李如忠2(1., 230009;2., 230009) :NO3--N、NO2--N,DB1;,NO3--N96.16%,NO3--N,100%;NO3--NNO2--N,NO2--N。:;;;:X523;P641 :A :1003-5060(2008)10-1565-04ExperimentalstudyonnitrateremovalfromgroundwaterbytheanaerobicstrainDB1isolatedfromthesewageLIUYong1, QIANJia-zhong2, LIYun-xia2, LIRu-zhong2(1.SchoolofBiotechnologyandFoodEngineering,HefeiUniversityofTechnology,Hefei230009,China;2.SchoolofResourcesandEnvironmentalEngineering,HefeiUniversityofTechnology,Hefei230009,China)Abstract:ThedenitrificationactivityofthestrainwasinvestigatedthroughdeterminingNO3--N,NO2--Nandconcentrationofthestraininmedium.ResultsshowthattheremovalefficiencyofNO3--Nreached96.16%inwater,andcompletedenitrificationwasachievedinsimulatedaquiferwiththeremovalefficiencyof100%;theremovalofNO3--NandaccumulationofNO2--Nmainlyhappenedintheexponentialphase.TheremovalofNO2--Nmainlyhappenedinthestableandincli-ningperiods.Keywords:denitrobacteria;anaerobicstrain;nitrateremoval;aquifer 2060。、,,(),,,[1-4]。。NO3--N,()[5],NO2-[6]。[7,8],,,,,[7],,[8],。、。、、,NO2-、NO、N2ON2[9],、[7]。,,,、、、、、[10]。,。,,[11],[12],。、,,,[13,14],,,。,,,。1 (1)。2mL100mL(10g,KNO31g,1000mL),32℃24h,5;1mL,;24h。(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g,20g,1000mL),32℃24h,3。,,2mL100mL(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g,1000mL),7d,。(2)。1000mL600mL(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g),2%,32℃,,、,3。(3)。10cm,,:1248.0g/cm3,0.33,。,,。55cm,2cm,,。,[15],,,,,,。30℃,2%。、。,。(4)。[16];N-(1-)-[17];[17];[18]。2 2.1 18,3:①、,;②、,、;③、、。4,1、2、3、4。,4,3,4。1、2、37d,1566 合肥工业大学学报(自然科学版)第31卷 75.6%、43.56%62.04%,1,DB1。2.2 DB1(1)DB1。DB1NO3--N、NO2--N,1。1 1,DB1、、。NO3--N,DB110h,34h,,NO3--N,NO2--N,DB1NO3--NNO2--N,36h,NO2--N,NO2--N,;60h,NO2--N,NO2--N,NO3--N。140hNO3--N96.16%。(2)DB1。,NO3--N、NO2--N,DB1,2。2 NO3--N72h146.3mg/L2.8mg/L,;NO2--N24h,102.1mg/L,24h,,NO3--NNO2--N100%。2NO3--N,NO3--N,,;NO2--N,;2,,140h120h,,;,,NO3-※NO2-※NO(g)※N2O(g)※N2(g),。3 (1)DB1,、、、,。(2)DB1,DB196.16%;,120h100%。(3)DB1,。[ ][1] ,.[J].,2004,(4):22-26.[2] , ,,.[J].,2004,19(2):183-191.[3] ,,.15N18O[J].,2001,20(4):95-108.[4] ,,,.1567 第10期刘 咏,等:从污水中分离出菌株DB1去除地下水硝酸盐实验[J].,2006,28(1):102-107.[5] HaugenKS,SemmensMJ,NovakPJ.Anovelinsitutechnologyforthetreatmentofnitratecontaminatedgroundwater[J].WaterResearch,2002,36:3497-3506.[6] KesseruP,KissI,BihariZ,etal.Biologicaldenitrificationinacontinuous-flowpilotbioreactorcontainingimmobilizedPseudomonasbutanovoracells[J].BioresourceTechnolo-gy,2003,87:75-80.[7] TangCY,AzumaK,IwamiY,etal.Nitratebehaviorinthegroundwaterofaheadwaterwetland,Chiba,Japan[J].HydrologicalProcesses,2004,18:3159-3168.[8] BohlkeJK,WantyR,TuttleM,etal.Denitrificationintherechargeareaanddischargeareaofatransientagricul-turalnitrateplumeinaglacialoutwashsandaquifer,Min-nesota[J].WaterResourcesResearch,2002,38(7):1105-1129.[9] OvezB,OzgenS,YukselM.BiologicaldenitrificationindrinkingwaterusingGlycyrrhizaglabraandArundadonaxasthecarbonsource[J].ProcessBiochemistry,2006,41:1539-1544.[10] ,.[M].:,2005:170-172.[11] SmithRL,BuckwaterSP,PepertDA.Small-scale,hy-drogen-oxidizing-denitrifyingbioreactorfortreatmentofnitrate-contaminateddrinkingwater[J].WaterResearch,2005,39:2014-2023.[12] RezaniaaB,OleszkiewiczaJA,CicekbN.Hydrogen-de-pendentdenitrificationofwaterinananaerobicsubmergedmembranebioreactorcoupledwithanovelhydrogendeliv-erysystem[J].WaterResearch,2007,41:1074-1080.[13] KimYJ,YoshizawaM,TakenakaS,etal.IsolationandcultureconditionsofaKlebsiellapneumoniaestrainthatcanutilizeammoniumandnitrateionssimultaneouslywithcontrolledironandmolybdateionconcentrations[J].BiosciBiotechnolBiochem,2002,66(5):996-1001.[14] SilvaSR,GingPB,LeeRW,etal.Forensicapplica-tionsofnitrogenandoxygenisotopesintracingnitratesourcesinurbanenvironments[J].EnvironForensics,2002,3(2):125-130.[15] , , .[J].,2004,24(3):504-507.[16] ,,.[M].:,2002:55-78.[17] ,, ,.[M].4.:,2003:211-274.[18] .[M].:,2005:70-72.( )(1564)(3),,。(4),,,。[ ][1] AslanS,CakiciH.Biologicaldenitrificationofdrinkingwa-terinaslowsandfilter[J].JournalofHazardousMaterials,2007,148:253-258.[2] .[M].:,1988.[3] DellaRoccaC,BelgiornoV,MeriS.Heterotrophic/auto-trophicdenitrification(HAD)ofdrinkingwater:prospec-tiveuseforpermeablereactivebarrier[J].Desalination,2007,210:194-204.[4] SchipperLA,Vojvodic-VukovicM.Nitrateremovalfromgroundwateranddenitrificationratesinaporoustreatmentwallamendedwithsawdust[J].EcologicalEngineering,2000,14:269-278.[5] , ,,./[J].,2006,22(16):16-19.[6] SchipperLA,Vojvodic-VukovicM.Fiveyearsofnitrateremoval,denitrificicationandcarbondynamicsinadenitrifi-cationwall[J].WatRes,2001,35(14):3473-3477.[7] ,,.[J].,2004,23(3):512-515.[8] SuChunming,PulsRW.Removalofaddednitrateincot-tonburrcompost,mulchcompost,andpeat:mechanismsandpotentialuseforgroundwaternitrateremediation[J].Chemosphere,2007,66:91-98.[9] .[M].4.:,2002:258-274.[10] SchipperLA,Vojvodic-VukovicM.Hydrauliccon-straintsontheperformanceofa
本文标题:从污水中分离出菌株DB1去除地下水硝酸盐实验刘咏
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