从亚硝酸还原厌氧氨氧化转变为硫酸盐型厌氧氨氧化刘正川

36920159ENVIＲONMENTALSCIENCEVol．36No．9Sep．2015*710055UASB．177d．130mg·L－1500mg·L－158.9%15.7%74.3mg·L·d－177.5mg·L·d－1、2pH．CandidatusbrocadiaBacillusbenzoevorans．．UASBX172X703.1A0250-3301201509-3345-07DOI10．13227/j．hjkx．2015．09．0292015-02-022015-04-062009ZX07212-0021988～[e-mail]*[e-mail]-ＲeducingAnaerobicAmmoniumOxidationＲeactorStartedwithNitrate-ＲeductingAnaerobicAmmoniumOxidationLIUZheng-chuanYUANLin-jiang*ZHOUGuo-biaoLIJingKeyLaboratoryofEnvironmentalEngineeringofShaanxiProvinceKeyLaboratoryofNorthwestWaterＲesourcesEnvironmentandEcologyMinistryofEducationSchoolofEnvironmentalandMunicipalEngineeringXi'anUniversityofArchitectureandTechnologyXi'an710055ChinaAbstractThetransformationofnitrite-reducinganaerobicammoniumoxidationtosulfate-reducinganaerobicammoniumoxidationinanUASBwasperformedandthechangesinmicrobialcommunitywerestudied．Theresultshowedthatthesulfatereducinganaerobicammoniumoxidationprocesswassuccessfullyaccomplishedafter177days'operation．Theremovalrateofammoniumnitrogenandsulfatewereupto58.9%and15.7%theremovingloadofammoniumnitrogenandsulfatewere74.3mg·L·d－1and77.5mg·L·d－1whileconcentrationofammoniumnitrogenandsulfateofinfluentwere130mg·L－1and500mg·L－1respectively．Thelostnitrogenandsulphurwasaround2inmolarratio．ThepHvalueoftheeffluentwaslowerthanthatoftheinfluent．InsteadofCandidatusbrocadiainnitritereducinganaerobicammoniumoxidationgranularsludgeBacillusbenzoevoransbecamethedominantspeciesinsulfatereducinganaerobicammoniumoxidationsludge．Thedominantbacteriuminthetwokindsofanaerobicammoniumoxidationprocessisdifferent．Ourresultsimplythatthetwoanaerobicammoniumoxidationprocessesarecarriedoutbydifferentkindofbacterium．Keywordsbiologicalnitrogenremovalnitritereducinganaerobicammoniumoxidationsulfate-reducinganaerobicammoniumoxidationUASBcommunitystructure1～4．N25～8．“”．2001Fdz-Polanco910．Zhang11Yang12SO2－4NH+4SO2－4NH+4．Liu13．1415．．Schrum16．1736．．、．．．11.110cm60cm6.3L．．35℃±1℃pH7.6±0.5HＲT=1d5．137.8mg·L－1165.7mg·L－194.8%93.2%．0.32kg·m3·d－1．1Fig．1Schematicdiagramofthereactor1.2．SS5487mg·L－1VSS4354mg·L－1MLVSS/MLSS0.79TN0.568kg·m3·d－1．NH4Cl、NaNO2Na2SO4KHCO3500mg·L－1KH2PO427mg·L－1CaCl2180mg·L－1MgCl2200mg·L－118．1.31.3.1、、19．pHMLSS、MLVSS、SEM．1.3.2DNAOMEGAE．Z．N．A．TMSoilDNAKitD5625-01．PCＲGC-968F5'-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTAC-3'1401Ｒ5'-CGGTGTGTACAAGACCC-3'．95℃5min94℃30s58℃45s72℃45s3572℃7min．PCＲ．DGGEDCodeTMUniversalMutationDetectionSystemU．S．ABio-ＲADCo．DGGE8%40%～70%100%7mol·L－140%15μLPCＲ1×TAE60℃130V10min80V12hGelＲed30min2021．PCＲ．22.1．100mg·L－170mg·L－120mg·L－1．23．14d82.4%99.6%、．．643392Fig．2Ｒemovalofammoniumnitrogenandnitritenitrogeninsulfatereducinganaerobicammoniumoxidation80mg·L－1、25mg·L－1200mg·L－1．3Fig．3Ｒemovalofsulfateinsulfatereducinganaerobicammoniumoxidation54.5%100%2.7%．29d80mg·L－1、200mg·L－119.2%61d35.5%12.7%Liu13、2、2.42．Fdz-Polanco22NO－2．．100mg·L－1、300mg·L－137d．126d48.6%18.6%、2.06．130mg·L－1500mg·L－1．48d177d74333658.9%15.7%74.3mg·L·d－177.5mg·L·d－1、2.192．N/S2∶1．2.2pHJetten23．．4．．8.36mg·L－1．NO－2．．24．105d174d8.7mg·L－1．4pHFig．4VariationofnitrateandpHinsulfatereducinganaerobicammoniumoxidationpHpHpHpH．25H+pH0～29d．44dpHpH．44～177dpHpH26．pH．2.310～29dMLVSS/MLSS0.73．61d90dMLVSS/MLSS0.01ΔG－45.35kJ·mol－1Zhang11ΔGΔG．．．177dMLVSS/MLSS0.77．1Table1Sludgecharacteristicsduringthetransformation/dMLSS/g·L－1MLVSS/g·L－1MLVSS/MLSS05.4874.3540.79295.2143.7980.73905.3723.9600.741775.2534.0520.7720.3～0.8mm．843392Table2Changeofsizeofgranularsludgeduringthetransformation/d/%＜0.3mm0.3～0.8mm0.8～1.0mm1.0～2.0mm＞2.0mm016.534.718.417.413.09018.337.915.716.211.917717.135.318.418.810.4SEM5ab177d2～3μm．．5Fig．5Sludgemorphologyofscanningelectronmicroscope2.4PCＲ-DGGE6．A、BDNADGGE．DGGEband1～band6PCＲ．GenBankBLASTband2CandidatusbrocadiasinicaAB565477100%GenBankUnculturedCandidatusbrocadiasp．cloneYLJ-1KM583866．Band1Bacillusbenzoevorans16SrＲNAgeneY14693100%GenBankUnculturedbacillussp．cloneYLJ-2KP336392．ClustalXMEGA16SrＲNA7．BLAST6band3Anaerolineaceae100%band4Bacteroidetes91%Band5Nitrospira6PCＲ-DGGEFig．6PCＲ-DGGEfingerprint96%band6Thiobacillus93%．DGGEband5band6band3、band4A、B943336716SrＲNAFig．7Molecularphylogenetictree．band1、band2BacillusCandidatusbrocadia．27Bacillus．Bacillus．Bacillus．UnculturedCandidatusbrocadiasp．cloneYLJ-1Unculturedbacillussp．cloneYLJ-216SrＲNAgene．31．177d．130mg·L－1500mg·L－158.9%15.7%74.3mg·L·d－177.5mg·L·d－1、2.19．pH．2CandidatusbrocadiaBacillusbenzoevorans．．1TsushimaIOgasawaraYKindaichiTetal．Developmentofhigh-rateanaerobicammonium-oxidizingANAMMOXbiofilmreactorsJ．WaterＲesearch20074181623-1634．2．J．20133493544-3551．3JinＲCYangGFZhangQQetal．TheeffectofsulfideinhibitionontheANAMMOXprocessJ．WaterＲesearch20134731459-1469．4．J．20143572834-2842．5VanDeGraafAAMulderADeBruijnPetal．AnaerobicoxidationofammoniumisabiologicallymediatedprocessJ．AppliedandEnvironmentalMicrobiology19956141246-1251．6SuminoTIsakaKIkutaHetal．NitrogenremovalfromwastewaterusingsimultaneousnitratereductionandanaerobicammoniumoxidationinsinglereactorJ．JournalofBioscienceandBioengineering20061024346