氮杂环化合物喹啉在缺氧条件下降解的影响因素程静

37920099()JOURNALOFTONGJIUNIVERSITY(NATURALSCIENCE)Vol.37No.9　Sep.2009:0253-374X(2009)09-1207-05DOI:10.3969/j.issn.0253-374x.2009.09.013:2008-05-28:(EREH0712):　(1978—),,,,.E-mail:[e-mail]程　静1,2,蔡伊秋1,张超杰1,周　琪1(1.,200092;2.,430070):pH、,.:--;pH7～9;;;.:;;;:X703　:AInfluenceFactorsofNitrogenousHeterocyclicCompound-QuinolineDegradationUnderAnoxicConditionsCHENGJing1,2,CAIYiqiu1,ZHANGChaojie1,ZHOU,Qi1(1.StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai200092,China;2.SchoolofCivilEngineeringandArchitecture;WuhanUniversityofTechnology,Wuhan430070,China)Abstract:Astudywasmadeoftheanoxicdegradationandremovalofnitrogenousheterocycliccompound-quinoline,denitrificationofacclimatedsludgeaswellastheeffectofpH,C/Nratio,bio-ferricondegradationofquinolineunderanoxicconditionsbyflask-shakingtests.Themicrobialdegradationproductsweremeasuredandthedegradationpathwayofquinolineinanoxicconditionwasanalyzed.Theresultsindicatethatquinolineisremovedbythreesteps:biosorption,desorptionandbiodegradation.TheoptimalrangeofpHisbetween7and9.TheC/Nratiodoesnotaffectthereductionofquinolineobviously.Bio-ferricprocesscannotimprovethedegradationofquinoline,quinolinecanbeusedasthesolecarbonandnitrogensourcesforbiodegradation.Keywords:nitrogenousheterocycliccompound;quinoline;anoxic;influencefactors　　,,C9H7N.—CH,..、、,、.,、,、.,..,[1-4].CuiComamonassp,[5];Fetzner,[6].//(AAO),[7];,[8].、,,.,,pH、,.　　　()37　1　1.1　,.25cm、20cm、5L.25℃～30℃,,..1.2　,,,,,、NaNO3,,K2HPO4·3H2O,CaCl2MgSO4、,,C∶N∶P∶Ca∶Mg=100∶20∶1∶0.18∶0.18.,.,2,.3000～3500mg·L-1.1.3　,,,3%～5%.,,,;100ml;,,,,0.1mg·L-1,.,25℃,160r·min-1..,,-.VARIANProstar(HPLC),UV-VIS,20μL,0.20μm,WatersXTerraRP18(250＊4.6mm,5μm),256nmVACN∶Vwater=40∶60,1ml·min-1,3.81min,.NO3—N,NO2—N,pH,(SS)、(VSS)[9].ThermoElectronCorporationGC/MS,,.HP,:60℃,2min,15℃·min-1,250℃3min.EI,70ev,250℃.SigmaBSTFA(GC/MS).2　2.1　,.,.150mg·L-1,8[10].12NO-3—NNO-2—N.1　NO-3—NFig.1　NO-3—Nvariationindenitrificationprocessofacclimatedsludge2　NO-2—NFig.2　NO-2—Nvariationindenitrificationprocessofacclimatedsludge1208　9　,:　　　　1,8,0.5h,0.5～3hNO-3—N,.,NO-3—N.NO-3—N.12,,NO-3—NNO-2—N,NO-2—N,,NO-3—N,NO-2—N,NO-2—N,NO-2—N.,,.2.2　,.,.150mg·L-1,8,,6h.3.3　Fig.3　Anoxicdegradationofquinolineindifferentmedia　　3,.1h,.,,,,,;31h,,,,,,.(1)(2),,NO-3—N,1h,1～3h,NO-3—N,NO-3—N,,NO-3—N.2.3　pH,1g3.57g,pH,,pH,pH.100mg·L-1pH(6h),,4.4　(100mg·L-1)Fig.4　Quinoline(100mg·L-1)variationindifferentpH　　4,pH6～9,.pH7～9,,pH7,,.pH6～9,,7pH6.5～7.5,:pH;,(TKN),.pH7,,pH.,pH6.5～7.5,pH7,.pH,,1209　　　()37　pH.pH.,pH7～9.pH,.2.4　,,,.,6h,NO3—N,NO2—N,1.1　Tab.1　Relationshipbetweenoutletquinolineandm(C)/m(N)ρ()/(mg·L-1)ρ(NO-x—N)/(mg·L-1)ρ()/(mg·L-1)ρ(NO-3—N)/(mg·L-1)ρ(NO-3—N)/(mg·L-1)/%1500.2514.310.350.8290.46150223.50216.2844.812.2689.15150111.75414.1622.462.4990.5615074.50614.2415.832.3290.5115055.88813.5811.332.6790.9515044.701014.389.422.4890.4215022.352014.404.282.5590.40　　　:ρ(NO-x—N)ρ(NO-3—N)ρ(NO-3—N);ρ(COD)/ρ(NO-3—N),ρ(COD)ρ(COD)(100mg·L-1ρ(COD)298mg·L-1).　　1,,,90%.,0.25mg·L-1,0.35mg·L-1,.,,7,.,,.(=20),,.:,,,;,TKN,,.,[11].,NO-2—N.2.5　,.,、,、.FeCl3Na2CO3,Fe3+,5.5　Fig.5　Quinolinevariationindifferentconcentrationsofbio-ferric　　5,.,,,.2.6　,GC/MS1h,3h15h,,,1210　9　,:　　2,8-,8-,.3h67.6　3hGC/MSFig.6　GC/MSspectraofsupernatantafterquinolinedenitrificationfor3h7　Fig.7　Degradationpathwayofquinolineinanoxiccondition　　,,,pH,pH7～9.,,,,.3　(1),NaNO3,--,,.(2)pH,pH7～9.(3),,.(4).(5),.:[1]　,,.[J].,2003,22(1):52.CUIMingchao,LILi,CHENFanzhong.Studyprogressonbiodegradationofquinolineanditsderivatives[J].ShanghaiEnvironmentalSciences,2003,22(1):52.[2]　,.[J].,2005,25(2):32.HONGXin,YANGXianghua.Isolationcharacterizationanddieseloildenitrogenofquinoline-degradingbacterium[J].JournalofLiaoningUniversityofPetroleum&ChemicalTechnology,2005,25(2):32.[3]　,,,.[J].,2006,26(5):1390.LIUBinbin,ZHANGFeng,FENGXiaoxi,etal.Identificationoffunctionallyimportantmicroorganismsinalab-scaleanaerobicbiofilmreactorforquinoline-degradation[J].ActaEcologicaSinica,2006,26(5):1390.[4]　WANGJianlong,WUWeizhong,ZHAXuan.Microbialdegradationofquinoline:kineticsstudywithburkholderiapickttii[J].BionedicalandEnvironmentalSciences,2004,17(1):21.[5]　CUIMingchao,CHENFanzhong,FuJiamo,etal.MicrobialmetabolismofquinolinebyComamonassp[J].WorldJournalofMicrobiology&Biotechnology,2004,20(6):539.[6]　FetznerS.Bacterialdegradationofpyridine,indole,quinoline,andtheirderivativesunderdifferentredoxconditions[J].AppliedMicrobiologyandbiotechnology,1998,49(3):237.[7]　,,.A1—A2—O[J].,2002,22(1):34.LIYongmei,GUGuowei,ZHAOJianfu.StudyonbiodegradationcharacteristicsofseveralnitrogenheterocycliccompoundsincokeplantwastewaterinA1—A2—Obiofilmsystem[J].ActaScientiaeCircumstantiae,2002,22(1):34.[8]　,,.[J].:,1999,8(3):34.HEMiao,YAOJun,ZHOUGuoqiang.StudyprogressonbiodegradationofPAHsandheterocycliccompounds[J].JournalofTianJinProfessionalCollege,1999,8(3):34.[9]　.[M].4.:,2002.MinistryofEnvironmentalProtectionofthePeople'sRepublicofChina.WaterandExhaustedWaterMonitoringAnalysisMethod[M].4thed.Beijing:ChinaEnvironmentSciencePress,2002.[10]　,,.[J].,2003,22(11):734.MANa