低浓度氨氮废水在ABR中的厌氧氨氧化研究朱月琪

4　6环境工程学报Vol.4,No.620106ChineseJournalofEnvironmentalEngineeringJun.2010ABR朱月琪1,2,3,4　张丽娟1,3,4　曾国驱1,3,4　许玫英1,3,4　孙国萍1,3,4＊(1.,510070;2.,510006;3.,510070;4.,510070)　　(anaerobicbaffledreactor,ABR)(anaerobicammoniumoxidation,anammox)。4,NH3-NNO2-N3.91g/(m3·d)3.21g/(m3·d),85.7%98.8%。(fluorescenceinsituhybridization,FISH)ABR。,HRTNH3-NNO2-N,NH3-NNO2-N65.53g/(m3·d)68.46g/(m3·d),76.3%91.3%,1.7～2.5mm。(SEM),ABR,。　　ABR　　　X703　　　A　　　1673-9108(2010)06-1224-07StudyonanammoxoflowconcentrationofammonianitrogenwastewaterinABRZhuYueqi1,2,3,4　ZhangLijuan1,3,4　ZengGuoqu1,3,4　XuMeiying1,3,4　SunGuoping1,3,4(1.GuangdongInstituteofMicrobiology,Guangzhou510070,China;2.FacultyofEnvironmentalScienceandEngineering,GuangdongUniversityofTechnology,Guangzhou510006,China;3.GuangdongProvincialKeyLaboratoryofMicrobialCultureCollectionandApplication,Guangzhou510070,China;4.GuangdongOpenLaboratoryofAppliedMicrobiology,Guangzhou510070,China)Abstract　Anaerobicammoniumoxidation(anammox)processwasstart-upinaanaerobicbaffledreactor(ABR)inoculatedwithanaerobicsludgeandriversludgeunderlowammoniaconcentrations.Thewaterqualityofeffluentwasstepwisereacheredtoastablelevelafter4months'enrichmentoperation.TheaverageremovalefficienciesofNH3-NandNO-2-Nwere85.7%and98.8%whenthevolumeloadingratewas3.91g/(m3·d)and3.21g/(m3·d)respectively.TheanaerobicsludgeinABRwasanalyzedbyfluorescenceinsituhybridiza-tion(FISH).ThentheloadingrateofthisABRsystemwasincreasedgraduallybyshorteningtheHRTorbyin-creasingNH3-NandNO-2-Nconcentrationsintheinfluent.Finally,whenthevolumeloadingrateofNH3-NandNO-2-Nwere65.53g/(m3·d)and68.46g/(m3·d),theaverageremovalefficienciesreached76.3%and91.3%,respectively.Sludgegranuleswiththediameterof1.7～2.5mmweredeveloped.Theenrichedgranu-larsludgeandtheinitialseedgranularsludgewereobseverdbyscanelectronicmicroscope(SEM),themicroor-ganismspeciesintheenrichedgranularsludgewithhighanammoxactivitybecamemuchsimplifiedcomparingtothoseoftheinitialseedgranule.Keywords　anammox;ABR;ammonianitrogen;nitritenitrogen:(863)(2006AA06Z322);(sytz2008):2009-06-16;:2009-08-06:(1983～),,,。E-mail:[e-mail]＊,E-mail:[e-mail]　　MulderVandegraff[1,2],,NH+4、NO-2,N2。,,,,。6:ABR。,,[3,4]、[5,6]、[7,8],。,(ABR)。、C/N。1　1.1　1.1.1　工艺流程和装置1。ABR16.8L。40cm,14cm,30cm,27.5cm,15.4L。4,5∶1,45°。(hydraulicretentiontime,HRT)。1　ABRFig.1　ABRexperimentdevice1.1.2　接种污泥3∶1。,[9]MLSS82g/L。1.1.3　废水水质,:NaHCO31250mg/L,MgSO4·7H2O300mg/L,CaCl2·2H2O180mg/L,CaCl2·2H2O180mg/L,KH2PO427mg/L,Fe-EDTA(EDTA5000mg/L,FeSO4·7H2O5000mg/L)1mL/L,(ZnSO4·7H2O430mg/L,CoCl2·6H2O240mg/L,MnCl2·4H2O990mg/L,CuSO4·5H2O250mg/L,NiCl2·6H2O190mg/L,H3BO314mg/L)1mL/L。pH7.7～8.4。1.2　1.2.1　水质分析方法[9],N-(1-)-[9]。pHpH(Mettlertoledo,MP225)。1.2.2　荧光原位杂交(FISH)分析[10]。CandidatusBrocadiaanammoxidansCandidatusKueneniastuttgartiensisDNAAMX820[11](),GGGCACTAAG-TAGAGGGGTTTT[11],Invitrogen。DAPI()。[10]。LEICADMRA2,LEICACW4000FISHLEICAQ500IW。1.2.3　颗粒污泥粒径分析--,:10mL;,100mL;10mL,;,;Qwin,。1.2.4　污泥样品电镜(SEM)观察:3%5h;(PBS),30%(10min/,2)、50%(10min/,2)、70%()、90%(15min/,1)、100%(15min/,1);(CaCl2)(15min/,3);(CaCl2)(15min/,2);;;。2　,4。ABR3:(1～30d)、(31～117d)、(118～129d)。,ABR,60d,(130～180d)。122542.1　,HRT48h,(NH4)2SO4NaNO270mg/L。NH3-NNO-2-N23。2.1.1　驯化初期,,;50%。N、,NH3-N,、,NH3-N,NH3-N。,,,。Chanchoi[12]3、UASB(up-flowanaerobicsludgeblan-ketreactor)SBR(sequencingbatchreactor),NH3-N。,,,,,NH+4。,NO-2-N。,,,,。。2.1.2　活性提高期1,,。,,,NO-2-N。,NO-2anammox,NO-2-N。,NH3-NNO-2-N。,,,。2.1.3　平稳期4,,NH3-NNO-2-N3.91g/(m3·d)3.21g/(m3·d)。,NH3-N96.7%,85.7%,NO-2-N99.8%,98.8%。NH3-NNO-2-N1∶0.94。4　ABRFig.4　EffectofABRinstablestageforammonianitrogenandnitritenitrogenremovalpH,pH(5)。[13]anammoxH+,pH。5　ABRpHFig.5　InfluentandeffluentpHvaluesofABRinstablestage12266:ABR,,,。,ABR。2.1.4　负荷提升期ABR4,,NH3-NNO-2-N。,(HRT)(NH3-NNO-2-N)6(1),10d,60d。1　ABRTable1　RunningconditionsofABRathigh-loadingstageHRT(h)(NH4)2SO4、NaNO2(mg/L)(g/(m3·d))(g/(m3·d))124708.178.052127015.1613.84367029.1930.6141214033.6534.9151221053.6153.5561228065.5368.46　　,HRT,NH3-NNO-2-N,。6,HRT48h24h(1)12h(2),NH3-NNO-2-N80%95%。HRT6h(3),NH3-NNO2-N,63.6%88.9%(6)。,HRT12h,。4、5、6,,,,。43,34NH3-NNO-2-N,86.3%95.1%(6)。,HRT,HRT。HRT12h,210mg/L(5),NH3-N78.3%,NO-2-N(99.0%)。280mg/L(6),NO-2-N(91.3%),NH3-N76.3%。,ABR50.00gNH3-N/(m3·d),62.50gNO-2-N/(m3·d)。8.28mgNH3-N/(mgVSS·d)。6　ABRFig.6　RunningeffectofABRunderdifferentconditions2.2　FISH,。7(DAPI100%)。7　Fig.7　Anammoxbacteriaaccountsfortotalbiomassbeforeandafterdomestication122747,4,ABR4.3%7.6%、6.0%、18.1%26.4%。。ABR、。,,、[14]。,,,,,。。,、,,。2.3　8。1.80mm。129d,ABR,1.48、1.03、1.351.27mm。,,。8　ABRFig.8　DiameterchangesofsludgegranulesinABRchambers1,60d,ABR,,1.89、2.08、1.702.47mm。Ahn[7,8]UASB(1～2mm)。、。,ABR:,ABR。[15]。,,[16]。,,、。,HRT48h12h,2.41×10-6m/s9.64×10-6m/s,1.98×10-6m/s7.92×10-6m/s,,、ABR,,。,,。,。Hu[17],38.0～98.5mmol/L5.4～12.0mmol/L,(6)2.12mmol/L4.06mmol/L,。,,、,。2.4　(SEM),,9(a)9(b),;;,。,9(c)9(d)。,、,。,12286:ABR,,;ABR,ABR。9　SEMFig.9　SEMimagesofgranularsludge3　　(1),4,ABR。、3。,3.91gNH3-N/(m3·d),85.7%;3.21gNO2-N/(m3·d),98.8%。,pH。(2)FISH。ABR4.33%7.6%、6.0%、18.1%26.4%,、,34。(3)ABR。65.53gNH3-N/(m3·d)68.46gNO2-N/(m3·d),76.3%91.3%,8.28mgNH3-N