低温等离子体杀菌的实验研究倪盈

3　11环境工程学报Vol.3,No.11200911ChineseJournalofEnvironmentalEngineeringNov.2009倪　盈1　陈永昌1　王明连2　俞　坚1(1.,100124;2.,100124)　　,。,,、、。,,、、,。　　　　X703.1　　　A　　　1673-9108(2009)11-1951-05Experimentalstudyofnon-thermalplasmasterilizationNiYing1　ChenYongchang1　WangMinglian2　YuJian1(1.KeyLaboratoryofEnhancedHeatTransferandEnergyConservation,MinistryofEducation,KeyLaboratoryofHeatTransferandEnergyConversion,BeijingEducationCommission,BeijingUniversityofTechnology,Beijing100124,China;2.VirusandPharmacologyLaboratory,BeijingUniversityofTechnology,Beijing100124,China)Abstract　Owingtotheproblemofresidualsofchemicalcompositionandenvironmentalpollutioncausedbytraditionalchemicalwatertreatment,newwatertreatmenttechnologiesshouldbedevelopedimminently.Inpres-entstudy,non-thermalplasmatechnologywasintroducedtoinvestigatesterilizationeffectagainstE.coliinwater.Experimentshadbeenperformedwithdifferentelectricalvoltage,waterdepth,gasesandelectrodefins.There-sultsshowedthatnon-thermalplasmahadgoodsterilizationeffectandcanbeusedconveniently,fast,safelyandwithoutresidualleft.Thusthenon-thermalplasmatechnologyissuitableforsterilizingdrinkingwaterorsewage.Keywords　watertreatment;non-thermalplasma;sterilization:(50676001);2008:2008-11-19;:2009-01-09:(1984～),,,。E-mail:[e-mail]　　,。。,80%1/3。[1]。、。,,。,[2]。,,。,、、,。,,。1　1.1　(、)(、),,。“、、”,[3]。,、[4]。:3(1):,、、。:、。(2):α、β、γ、X。(3):,,。(4):[5]。(5):,。(6):,,,[6]。1.2　,。,::,,。,,,。:,。,,,。:。DNA。:,、,[7]。1.3　[8],、。,,30min99.9%。,2h70%,。Kelly[9](OAUG-DP),30s,6,。,。,。Marsili[10]、,,。,、、。28kV,。100%。10,,。,20,、、。,、、。2　,,。、(12cm×12cm×14cm)(、),1。,,2mm,2。1　Fig.1　Experimentaldeviceofplasmasterilization195211　:2　Fig.2　Structureofpositiveelectrode,,0.5×1～3μm。,,。E.coli285。,LB,37℃,16h。,,。,,100μL。,,,。cfu(colonyformationunit)。,。:(%)=C0-CC0×100%:C0———(cfu/mL);C———(cfu/mL)。,,。3　3.1　,5min,。3。,5mm5cm。,,。,3　Fig.3　Influenceofvoltageonsterilizationrate,,,,。。,,4。30kV,5mm。4(a),,4(b)10min。。4　Fig.4　Pictureofplasmaonbactericidaleffect,,5,5(a),5(b)10min。,。5　Fig.5　PictureofE.coliwithouttreatment19533,,,。3.2　,,,。,30kV,,。6。6　Fig.6　Changesofsterilizationrateofplasmawiththicknessofwater,,。,,,。3.3　,。。4(7),8。,4。,,,,。3.4　,,。917kV,5mm,。9　Fig.9　Influenceofdifferencegasesonsterilizationeffect,。,195411　:。[11,12]3s23p6,15.755eV。2s22p3,14.545eV(1),,,(946kJ/mol),。24.3123eV,,,,,。,,,,,。1　Table1　Basicconfigurationandionizationenergyofcommonatoms(eV)(eV)N2s22p314.54529.602Ar3s23p615.75527.630O2s22p413.61835.1183.5　,,。,。,1℃,,、。,,。,1.5W。,。:、,,。,,,,,。4　　,,,。30kV、5mm15min99%。,、。[1],..:,2002.19～20[2],,..:,2003.109～120[3],,..,2006,25(3):23～25[4],..,2006,(1):56～67[5],,.().,2000,(21):4～19[6]RansomC.J.,WallaceThornhill.Plasma-Generatedcra-tersandspherules.IEEETransactiononPlasmaScience,2007,35(4):828～831[7]DustinL.Sullivan,MarkA.Kemp,ScottD.Kovaleski.Characterizationofaferroelectricatmosphericpressureplasmasource.IEEETransactiononPlasmaScience,2007,35(5):1480～1485[8],,..,1998,8(2):78～80[9]KellyW.K.,MentieT.C.,BrickmanC.,etal.Roomtem-peraturesterilizationofsurfacesandfabricswithaoneat-mosphereuniformglowdischargeplasma.Ind.Microbiol.Biotechnol.,1998,20:61～69[10]MarsiliL.,EspieS.,AndersonJ.G.,etal.Plasmainac-tivationoffood-relatedmicroorganismsinliquid.RadiationPhysicsandChemistry,2002,65(2):507～513[11]..:,2000.285～286[12]RadzigA.A.,SimovB.M.Referencedataonatoms,molecules,andions.SpringerSeriesinChemicalPhys-ics,1985,31:88～1231955