滇池水华束丝藻Aphanizomenonflosaquae对低氮的生理响应

J．LakeSci．2014261131-136http//[e-mail]2014byJournalofLakeSciencesAphanizomenonflos-aquae*1211311＊＊143007221000493430010Aphanizomenonflos-aquae．．0．5mg/L．BG-113d50%72%．7d43d30mg/L．PhysiologicalresponsetolownitrogenlevelinAphanizomenonflos-aquaefromLakeDi-anchiWUYanlong12ZHENGLingling1LILin1YINDacong3DAIGongyuan1＆SONGLirong11StateKeyLaboratoryofFreshwaterEcologyandBiotechnologyInstituteofHydrobiologyChineseAcademyofSciencesWuhan430072P．Ｒ．China2UniversityofChineseAcademyofSciencesBeijing100049P．Ｒ．China3WaterＲesourcesDepartmentYangtzeＲiverScientificＲesearchInstituteWuhan430010P．Ｒ．ChinaAbstractTwostrainsofAphanizomenonflos-aquaewereisolatedfromspringcyanobacterialbloomsinHaigengBaywhichlocatedinthenorthernpartofLakeDianchi．BoththegrowthcharacteristicsandheterocystformationaswellasnitrogenfixationabilityofA．flos-aquaewereinvestigatedunderdifferentlowconcentrationsofsodiumnitrateandinnitrogen-freeculturemediumrespective-ly．TheresultsshowedthatA．flos-aquaestrainswereabletogrowintheBG-11mediumwithvariouslowconcentrationsofnitrateandthebiomassreachedaveryhighlevel．Thebiomassincreasedwiththenitrate-nitrogenconcentrationincreasingbuttherewasnosignificantdifferencewhennitrate-nitrogenconcentrationwasabove0．5mg/L．InductionofheterocystdifferentiationwasrapidwhenA．flos-aquaewasculturedinN-deficientBG-11mediumandafterreachingamaximumof72%approximatelythefilamentwithheterocystbegantoswinglower．Thetotalnitrogeninnitrogen-freemediumincreasedgraduallyasnitrogenfixationproceedandnitrogenconcentrationwascloseto30mg/Latday43．KeywordsLakeDianchiAphanizomenonflos-aquaenitrate-nitrogenheterocystnitrogenfixation1．、Microcystis2．＊＊＊NSFCU-0833604、2013ZX07102-00551279011．2013-02-252013-04-22．1984～[e-mail][e-mail]．2014261Aphanizomenonflos-aquae、、3-6．．1982121983147．198167．73×107cells/L98%“”8．1998340．5m9．2002———4m10．2001200911-12．、、、、、、4613-15．、16-20．、、．、．．11．1Aph120104Aph920113．1L30ml50ml．OlympusCX315CT213CT12．20±1℃、12h∶12h、20μE/m2·s．1150mlCT．．24°56'43″N102°38'55″E15．1±0．36℃、pH8．92±0．42、7．65±0．14mg/L、28．5±2．0cm、a121．2±39．6μg/L、0．027±0．009mg/L、0．037±0．010mg/L、0．332±0．056mg/L、2．933±0．178mg/L、4．360±1．594mg/L．1．2CT．3BG-1122、20±1℃、12h∶12h、30μE/m2·s．．90011001800680nm．OlympusCX31．．1．3pH、YSI550AYSIUSA23．1+948h．Aphanizomenonflos-aquae1331．4Origin8．0SPSS18．0One-wayANOVAP＜0．05．22．1Aph1Aph90、0．5、2、5、10、20mg/LBG-11274mg/LBG-110．1．．．Aph140d680nm1．35420、10、52mg/L45dBG-11P＞0．050．5mg/LP＜0．05．0．9．Aph1Aph945dP＜0．05．1Fig．1Theeffectsofdifferentnitrate-nitrogenconcentrationsonthegrowthofA．flos-aquaeisolatedfromLakeDianchi2Fig．2HeterocystcharacteristicsofA．flos-aquaeinducedinN-freestatus2．2．Aph93d50%7d72%．Aph1Aph950%．BG-1110d2．3134J．LakeSci．20142613Aph9Fig．3LightmicroscopeofheterocystformationatdifferentstagesandautofluorescenceofA．flos-aquaestrainAph94Fig．4NitrogenfixationcharacteristicsofthetwostrainsofA．flos-aquae．Aph93A～DBG-113E．Aph93F．2．3Aph1、Aph9．4．1Aphanizomenonflos-aquae1357d．．25mg/L．3、aDNA．a2425-26．160、、、、．．NH3α-Ca2+．N2NH3NH3-27-29．BG-113d50%．BG-110．5mg/LBG-11．0．5mg/L．30．Schindler31-34、、、30．、、35．30mg/L．--．、、．．41．．2008322286-289．2．———．20093361140-1144．3CarmichaelWWDrapeauCAndersonDM．HarvestingofAphanizomenonflos-aquaeＲalfsexBorn．＆Flah．var．flos-aquaeCyanobacteriafromKlamathLakeforhumandietaryuse．JournalofAppliedPhycology200012585-595．4TsujimuraSIshikawaKTsukadaH．EffectoftemperatureongrowthofthecyanobacteriumAphanizomenonflos-aquaeinLakeBiwaandLakeYogo．PhycologicalＲesearch2001494275-280．5ＲückerJStükenbANixdorfaBetal．Concentrationsofparticulateanddissolvedcylindrospermopsinin21Aphanizome-non-dominatedtemperatelakes．Toxicon200750800-809．6vegesVTapolczaiKKrienitzLetal．PhotosyntheticcharacteristicsandphysiologicalplasticityofanAphanizomenonflos-aquaeCyanobacteriaNostocaceaewinterbloominadeepoligo-mesotrophiclakeLakeStechlinGermany．Hydrobiologia20126981263-272．136J．LakeSci．20142617．、、．198594359-364．8．．2001216540-544．9．19802006．20072535-10．10．．201035243-46．11LiuYMChenWLiDHetal．FirstreportofaphantoxinsinChina-waterbloomsoftoxigenicAphanizomenonflos-aquaeinLakeDianchi．EcotoxicologyandEnvironmentalSafety20066584-92．12．．2005272172-176．13YamamotoYNakaharaH．TheformationanddegradationofcyanobacteriumAphanizomenonflos-aquaebloomstheim-portanceofpHwatertemperatureanddaylength．Limnology200561-6．14SuikkanenSKaartokallioHHllforsSetal．Lifecyclestrategiesofbloom-formingfilamentouscyanobacteriaintheBal-ticSea．DeepSeaＲesearchPartⅡTopicalStudiesinOceanography2010573/4199-209．15YamamotoYNakaharaH．LifecycleofcyanobacteriumAphanizomenonflos-aquae．Taiwania2009542113-117．16VahteraEConleyDJGustafssonBGetal．Internalecosystemfeedbacksenhancenitrogen-fixingcyanobacteriabloomsandcomplicatemanagementintheBalticSea．AMBIO2007362186-194．17ConleyDJPaerlHWHowarthＲWetal．Controllingeutrophicationnitrogenandphosphorus．Science20093231014-1015．18OliverＲLGanfGG．Freshwaterblooms-Theecologyofcyanobacteria．NetherlandsKluwerAcademicPublishers2000149-194．19WoodSAPrenticeMJSmithKetal．Lowdissolvedinorganicnitrogenandincreasedheter