电析吸附法提取动物毛中精氨酸和赖氨酸工艺黄玉秀

　:1998-09-07　,,1938,,;:.黄玉秀　刘　静　林伦民(　　510641)　　,5%Na2SO4,pH=3.0,400cm2,,3A/㎡,24h,,,.90%.,95%,,.　电析;吸附;氨基酸　TQ151.52,70%～80%,,.[1],pH[2],、,[3],.pH,,,,pH=3～5,,-〔+〕98%～99.99%,,,,.0.40%～4.00%,95.00%.,,.1　实验部分(1):;(2):,,,,;(3):;(4):::(CH2)4H2NCH(NH2)COOH(自然科学版)272JournalofSouthChinaUniversityofTechnologyVol.27　No.219992(NaturalScience)February　1999:(CH2)4H+3NRCHH+3NCOOHKa3H+(CH2)4H+3NCHH+3NRCOO-Ka2H+(CH2)4H+3NR±CHH2NCOO-Ka1H+(CH2)4NH2R-CHH2NCOO-:Ka1=〔R〕〔H+〕〔R〕,Ka2=〔R±〕〔H+〕〔R〕,Ka3=〔R-〕〔H+〕〔R±〕:Ka1=10-2.18,Ka2=10-8.95,Ka3=10-10.50:〔R〕=Ka1〔R〕〔H+〕=10-2.18〔R〕〔H+〕〔R±〕=Ka2〔R〕Ka1〔H+〕〔H+〕=10-11.13〔R〕〔H+〕2〔R-〕=Ka3〔R±〕〔H+〕=Ka3〔H+〕·10-11.13〔R〕〔H〕2=10-21.66〔R〕〔H〕3〔R〕:〔R〕=Ka1〔R〕/〔H+〕Ka1〔R〕〔H+〕+Ka1Ka2〔R〕〔H+〕2+Ka1Ka2Ka3〔R〕〔H+〕3+〔R〕=10-2.18/〔H+〕〔R〕〔R〕(10-2.18〔H+〕+10-11.13〔H+〕2+10-21.66〔H+〕3+1)=10-2.18/〔H+〕10-2.18/〔H+〕+10-11.13/〔H+〕2+10-21.66/〔H+〕3+1A=10-2.18/〔H+〕+10-11.13/〔H+〕2+10-21.66/〔H+〕3+1〔R〕=10-2.18〔H+〕A·100%〔R±〕=10-11.13/〔H+〕2A·100%〔R-〕=10-21.66〔H+〕3A·100%〔R〕+〔R〕+〔R±〕+〔R-〕=1〔R〕+10-2.18〔H+〕〔R〕+10-11.13〔H+〕2〔R〕+10-21.66〔H+〕〔R〕=1〔R〕(1+10-2.18/〔H+〕+10-11.13/〔H+〕2+10-21.66/〔H+〕3)=1〔R〕A=1〔R〕=1A·100%,,,pH,,pH=3.0,———〔+〕:66　　　27　〔+〕=〔R〕+〔R〕〔R〕=10-2.18/〔H+〕10-2.18/〔H+〕+10-11.13/〔H+〕2+10-21.66/〔H+〕3+110-11.13/〔H+〕210-21.66/〔H+〕3.〔R〕=10-2.18/〔H+〕10-2.18/〔H+〕+1·100%〔H+〕=0.001MHCl,pH=3.0〔R〕=10-2.18/0.00110-2.18/0.001+1·100%=6.60697.6069·100%=86.85%〔R〕=1A·100%=17.6069·100%=13.14%,〔+〕=〔R〕+〔R〕=(86.85+13.14)%=99.99%,pH=3.0Na2SO4,99.99%-〔+〕,,,.,.,,95.0%,,.(5):→→→→→→→.2　结果与讨论2.1　pH值、有效正电荷与渗析时间关系,,〔R〕〔R〕pH,,pH,pH,〔R〕,〔R〕,,pH,1.1　pH、Table1　RelationofpH、chargewithdialyzsistime(g)10101010101010(g)10101010101010pH1234567〔R〕(%)　93.80　60.21　13.15　　1.49　　0.15　　0.01　　0.00001〔R〕(%)6.1939.7886.8598.5199.8499.9899.99(h)1.52.03.04.04.54.64.8(%)99.5595.0095.0092.0090.0088.5085.00(%)95.0094.5093.5091.0090.0088.5085.502.2　膜面积对渗析速度的影响,,,　2:67　　　〔R〕〔R〕,,,2.2　Table2　Influenceoffilmareafordialysespeed(g)10101010101010(g)10101010101010(m2)2040801603206401280(h)　10.00　　5.00　　2.50　　1.20　　0.56　　0.28　　0.14(%)95.0096.0096.0095.8096.0096.2096.00(%)95.1095.4095.8095.4095.9095.8096.102.3　电流密度对渗析速度的影响,,D=3A/m2,,3.3　Table3　Influenceofcurrentdensityfordialysespeed(g)10101010101010(g)10101010101010(A/cm2)　　2.0　　3.0　　4.0　　4.5　　5.0　　5.5　　6.0(h)1.501.001.651.802.102.402.70(%)95.5096.0095.8096.2096.8096.5096.00(%)95.3095.9096.0096.4096.8096.0096.302.4　赖氨酸和精氨酸浓度对渗析速度的影响,,,4.4　Table4　InfluenceofLysineandArginineconcentrationfordialysespeed(g)10101010101010(g)10101010101010(h)　　1.5　　3.0　　6.0　　12.0　　24.0　　48.0　　96.0(%)95.8095.3096.5096.1096.3096.5096.05(%)95.0095.8096.0096.6096.9096.8096.003　结　论,,5%NaSO4,pH=3.0,400m2,D=3A/m2,24h,、,,,.:0.50%～1.5%,92.00%;0.40%～4.00%,95.00%.,,,,,,.68　　　27　　　　1　,..,1996,13(3):58～602　,,.L_L_,,1984(4):1～33　..,1985(2):21～25TECHNOLOGYOFPRODUCTIONOFARGININEANDLYSINEINTHEMETHODOFELECTRODIALYSISABSORPTIONHuangYuxiu　LuJin　LinLunmin(DepartmentofAppliedChemisytry,SouthChinaUniv.ofTech.,Guangzhou,510641)Abstract　MixedAminosolutionissolutedinNa2SO4systeminelectrodialysispoolwith400cm2areofmembranelinthemethodofelectrodialysisabsorption.pH=3.0,connectpower,powersupply(3A/m2)turnintoelectricfieldaftertwohourselectrodialysissothatacidscapacityaminomovetocathode,basiscapacityaminomovetoamode,reststillstayedinsolution.90%ofbasicandacidscapacityaminocanbemoved,andthenbasiccapacityaminothroughactivecharcoal,95%ofArgininecanbeabsorpted,butLysinecannotbeabsorptedsothatthegoalofseparationandpurificationbegot.Keywords　electrodialysis;adsorption;aminoacids　2:69