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[J].,2006,28(6):140-141.[2]DasS.ImprovingtheperformanceofSAGD[C].SPE/PS-CIM/CHOA97921,2005SPEInternationalThermalOperationsandHeavyOilSymposium,Alberta,Canada,2005.[3]KnollR,YeungKC.DrillingengineeringchallengesincommercialSAGDwelldesigninAlberta[C].SPE62862,2000SPE/AAPGWesternRegionalMeeting,LongBeach,California,2000.[4].[J].,2005,12(5):9-13.[5].[J].,1994,13(2):33-35.[6],,,.-[J].,2007,26(1):51-54.[7].BH-1[J].,2000,19(5):33-34.[8],,.O/W[J].:,1997,21(6):82-84.[9],,,.[J].,2007,27(6):61-63.[10],,,.[J].,2008,28(6):56-58.[](1977—),2007(),,。:010-62097457,E-mail:logical_2008@163.com。[]2009-05-05()StudyonthecultureofcomplexmicrobialflocculantproducedbybacteriawithstarchwastewaterWangYoule,ZhangBaorong,FanZhiming,HouJian(CollegeofEarthandEnvironmentalScience,LanzhouUniversity,Lanzhou730000,China),,,(,730000)[]M9M17,。、。:COD1600mg/L,0.3g/L、0.04g/L,pH,M960mL/L、M1740mL/L35h。,5mL/L92.67%。COD510mg/L,93.60%,,,。[];;;[]X798;TQ314.253;Q939.9[]A[]1005-829X(2009)10-0055-05Abstract:Flocculationcharacteristicsofcomplexmicrobialflocculant(CMBF)producedbyRhizopussp.M9andM17havebeenstudied,andthecultureconditionsofthetwostrainsusingpotatostarchwastewaterasnutrientmediumareoptimized.TheCMBFischaracterizedbylowdosage,betterflocculationefficiencyandheatresistance.Theoptimizedcultureconditionsofthecomplexbacteriawithpotatostarchwastewaterareasfollows:ThewastewaterCODisabout1600mg/L,0.3g/Lofureaand0.04g/Lofpotassiumdihydrogenphosphate,withoutaddingcarbonsourceandadjustingpH.Afterinoculating60mL/LofM9and40mL/LofM17,culturefor35h.Undertheseconditions,adding5mL/Loffermentationbrothtokaolinsuspensioncanmaketheflocculatingratereach92.67%.TheCODofbacteriaculturedwastewaterisonly510mg/Lwitharemovalrateof93.60%.Itcanbeusedeitherfordirectaerobicdischarging,reachingthestandard,orforirrigatingpotatocultivationbaseaftermixedwithfreshwater.Itlowersgreatlythedifficultyoftechnologicaltreatment.Keywords:potatostarchwastewater;complexmicrobialflocculant;inexpensiveculture;flocculationrate!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!2910200910IndustrialWaterTreatmentVol.29No.10Oct.,200955——2009-10,29(10)(MBF),、、、DNA〔1〕,,、、、,,,〔2〕。,MBF(CMBF),〔3〕。。,,,〔3〕、〔4〕、〔5〕、〔6〕。〔7〕,,,,COD5000~17000mg/L。,,。11.1(1)。、、4。(2)。pH4~6,pH,,、(PDA)。:100mL,C、N、P,。(3)。,、、,1、,1h。1。1.2CH20,DZKW-D-4,YX·280B,HZQ-X100A,DHG-9145A,JJ-4A,LD5-2A。22.1、,PDA,28℃150r/min2d100mL(75μm,5g/L,),,,,5。2.25,,1mL50mLPDA(,),2d,。(1)。PDA2d,3000r/min10min,(A)、(B)、(C)(D)。(2)。,2,,3000r/min10min,,,60℃。(3)。10min。(4)。MolishMBF;、MBF、。2.3,,,COD,、、,,pH,,。2.4(1)。CODCrET99731COD;BOD5ET99724A-6BOD;pHHI9224pH;HI93703-11;1CODCr/(mg·L-1)BOD5/(mg·L-1)pH/NTU/796523606.047126456——2009-10,29(10)。(2)。100mL,1mL10%CaCl2,300r/min0.5min,150r/min5min,10min,,1cm。33.180%28,15。5,7。M9+M17,0.2mL/L,95.46%。M9,,,,;M17,,,。,,,,《》〔8〕,M9M17。3.2,1。1,,(A),,;(D);(B),,(C),。3.3,CMBF1.23g/L。3.410CMBF10min,0.2mLCMBF,,1h91%,,。3.5Molish,,;、、。CMBF,,。3.63.6.1,。,,,。,。3.6.22,、0.5mL10g/L、0.5mL10g/L,2mL50mL,28℃、150r/min2d,0.1mL,2。2,,,,、,,。3.6.3COD,、、,COD、50mL、、,2mL。2。2,COD,,:57——2009-10,29(10)2COD/(mg·L-1)/mL/mL/mL/%1796500081.43279650.50.50.273.14379651.01.00.566.14479651.51.51.060.715398300.50.580.28639830.501.062.85739831.01.5072.57839831.51.00.273.439199101.01.088.861019910.51.50.580.851119911.000.279.711219911.50.5079.0013159301.50.290.431415930.51.0081.861515931.00.51.077.711615931.500.579.88281.4341.0303.9314.9289.1298.7310.1316.7328.4296.1310.3307.2329.9293.0304.6290.1R48.548.06.426.6(M9/M17)/mL/%10h20h25h30h35h35h40h48h1.0(0.6/0.4)60.4365.2882.0086.1488.2885.8580.2887.282.0(1.2/0.8)62.1465.5782.7186.7188.6386.1480.5787.853.0(1.8/1.2)66.7167.7182.8587.8589.8689.4282.4287.574.0(2.4/1.6)69.2870.8685.5788.5791.3589.2882.8589.005.0(3.0/2.0)75.1472.1487.0090.4292.6790.7185.4290.287.5(4.5/3.0)79.0075.4388.2891.7191.5891.2885.4290.5710.0(6.0/4.0)81.7184.0091.2892.4189.7292.2886.1492.2815.0(9.0/6.0)82.8686.5791.8593.5487.2193.9786.4292.71/mL4.01.01.00.50.50.20.050.053、100mL,C、N、P,,,,,;COD,,C、NP,。R,CODC,P,N。50mLCOD1600mg/L1.5mL10g/L(0.3g/L)、0.2mL10g/L(0.04g/L),,,2d,100mL0.1mL,90.43%。3.6.45mL,,2d3。3,,;M17M9,,,。V(M9)∶V(M17)3∶2,90.41%。3.6.5pHpH,4。4,pH,4.5~7.5,85%,92.14%(pH=5.5),pHpH,pH。pH,。3.6.6、50mL,、,3。3,。,,;,,,,,,,。,58——2009-10,29(10)MBF/(g·L-1)/(·g-1)/(mL·L-1)/%PDA1.230.8620.295.460.690.0140.592.714PDA[](1955—),1981,,。:13609368936,E-mail:wangyoule@lzu.edu.cn。[]2009-05-05()pH,:(1),;(2)pH,pH;(3):,、pH,,,,,,pH。,pH,。、。()pH———,,,.CN101264961.···············································,。〔9〕,,。,5mL(M93mL,M172mL)、35h、5mL/L,,92.67%。3.6.7,,,,COD,。PDA(2d)4。,,COD510mg/L,93.60%;122NTU,82.87%。4(1)M9M17,0.2mL/L,95.46%。(2)CMBF,1.23g/L。(3)CMBF,1h,4%。(4),COD1600mg/L,0.3g/L、0.04g/L,pH,M93mL、M172mL50mL35h,5mL/L,92.67%。(5),,COD93.60%,82.87%,,。[][1],,.[J].,1995,35(2):121-129.[2],.[M].:,2005:332-410.[3],,,.[J].,2007,23(1):38-42.[4],,,.HHE-P7[J].,2006,7(3):40-44.[5],,,.[J].,2006,26(9):29-32.[6],,.[J].,2007,23(9):19-23.[7].[J].,2007():62-71.[8].[M].:,1979:62-63.[9]GongWenxin,WangShuguang,SunXuefei,etal.BioflocculantproductionbycultureofSerratiaficariaanditsapplicationinwastew
本文标题:淀粉废水培养复合型微生物絮凝剂产生菌研究王有乐
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