发酵液中三油酸甘油酯污染超滤膜的清洗研究祁伟力

发酵液中三油酸甘油酯污染超滤膜的清洗研究祁伟力1,曹广祥2,钟传青2,姬胜利1＊(1.,250012;2.,276000)　:为解决发酵液的超滤过程中,超滤膜通量随着使用时间延长急剧下降的问题,通过加入亚硫酸氢钠,改变三油酸甘油酯性质,然后再加入EDTA+NaOH+SDS联合清洗,通量恢复率为93.68%.改进了传统的洗涤方法,提高了生产效率、降低了生产成本.:超滤膜污染;三油酸甘油酯;亚硫酸氢钠;清洗方法:TQ028.8　　:B　　:1007-8924(2009)02-0087-03　　,,.,,.[1].,、、,[2,3].,、.,、、EDTA(SDS),.,.SDS1.5%,16.93%.,,,,.1　实验部分1.1　:KOCH,,8088,:3000,:1000m2/×2.:.:、、EDTA、SDS,,.1.2　KOCH.(28±2)℃、0.2MPa,30min,Jwi52.6L/(m2·h).,,,Jwf8.3L/(m2·h).,Jwc.,FR(FluxRecovery)[4]:FR=(Jwc-Jwf)/(Jwi-Jwf).1[5].,3.24,DSDS1.5%E,F,0.6%NaHSO3,30min,E30min.　　3,0.4MPa,:2007-10-21;:2008-02-22:(04C26213701255):(1977-),,,,.＊〈[e-mail]〉29　2　　　　　Vol.29　No.220094MEMBRANESCIENCEANDTECHNOLOGYApr.2009DOI:10.16159/j.cnki.issn1007-8924.2009.02.006表1　清洗剂使用说明Table1　UsageofthecleaningagentNaOHEDTASDS/℃28404040表2　清洗剂编组配比Table2　ThecomposingofcleaningagentABCD1.5%0.8%NaOH0.8%NaOH+0.5%EDTA0.8%NaOH+0.5%EDTA+0.3%SDS1,30min,,.2　实验结果与讨论2.1　13,,,.DA,B,C3,,16.48%.4、,,,.表3　A,B,C和D4组清洗剂清洗的效果Table3　EffectofcleaningagentA,B,CandDonflux(n=5,x±s)ABCD/(L·m-2·h-1)9.60±1.6511.87±1.8713.50±1.9215.60±2.04FR/%2.938.0611.7416.482.2　2,,,,.:,885.5,,.,,1.5%,1.16.93%,D0.45%,.2.3　3,.SDS,,,,.,,,.NaHSO3.NaHSO3,,,.F0.6%NaHSO340℃30min,E,1.NaHSO3EDTA+NaOH+SDS,49.80L/(m2·h),93.68%,.,,,.1　D,EFFig.1　EffectofcleaningagentD,EandFonflux3　结论,,,,.,.,.NaHSO3,SDS,　·88　　　·　　　　　29　93.68%.[1],,.[J].,2006,26(5):1-15.[2].[J].,1999,2:28-30.[3],,,.[J].,2004,4(4):25-27.[4]SayedRazaviSK,HarrisJL,SherkatF.Foulingandcleaningofmembranesintheultrafiltrationoftheaqueousextractofsoyflour[J].JMembrSci,1996,114:93-104.[5].Ultra-floC[J].,2006,25(9):566-567.CleaningmethodforultrafitrationmembranefouledbyglyceroltrioleateinfermentationsolutionQIWeili1,CAOGuangxiang2,ZHONGQuanqing2,JIShengli1(1.SchoolofPharmacy,ShandongUniversity,Jinan250012,China;2.DepartmentofResearchandDevelopment,LunanPharmaceuticalGroupCorporation,Linyi276000,China)Abstract:Tosolvetherapiddeclineoffluxintheprocessoffermentationsolutionultrafiltration,sodiumbisul-phitewasappliedtochangethepropertyofglyceroltrioleateonmembrane,andthenthemembranewascleanedwithEDTA+NAOH+SDS,thefluxrecoveryisupto93.68%,comparingthe9%～16%fluxrecoveryfortraditionalcleaningmethod.Theproductionefficiencyincreasedandtheproductioncostdecreased.Keywords:ultrafiltrationmembranefouling;glyceroltrioleate;sodiumbisulphite;cleaningmethod(80)Studyonmembranediffusivityofchitosan/alginatemicrocapsulesZHANGHongliang,XUEWeiming,ZHAOBinran,LIUBo,SHIMei(CollegeofChemicalEngineering,NorthwestUniversity,Xi'an710069,China)Abstract:Basedonthree-dimensionalculturesystemofcellmicroencapsulation,usingchitosanandsodiumal-ginateasthemicro-carrierpreparationmaterials,thealginate-Cagelbeadsarepreparedbytheimpulsiveelec-trostaticpreparationprocess,thenthealginate-Cagelbeadsreactwiththesolutionofchitosanbypolyelec-trolyteinterconnection,sotheselectivepermeabilitymembraneofmicrocapsuleismade.Glucoseandcitricacidarethenecessarycarbonsourceforthecellculture,andL-glutamicacidisthenitrogensource.Inthiswork,usingthesesubjectsasthelowmoleculemodels,andaseriesofpoly(ethyleneglycol)s(PEG)ofdifferentmolecularmassasthehighmoleculemodelstostudythemolecularmasscut-off(MMCO)thediffusivityofchi-tosan/alginatemicrocapsuleswereinvestigated.Experimentalresultsshowthatglucose,citricacidandL-glu-tamicacidcandiffusethroughthemembranefreely,andthegelstructureinthemicrocapsulesisdepolymer-izatedbycompetitivelychelatingCa2+withtheingressofcitricacidandL-glutamicacid.Themolecularmasscut-off(MMCO)ofmicrocapsulespreparedwith20mg/mLsodiumalginateandfiftythousandmolecularmass,2mg/mLchitosanis1500.Keywords:sodiumalginate;chitosan;microcapsules;diffusivity;molecularmasscut-off(MMCO)　2:·89　　　·