发酵种泥的投加对新鲜剩余污泥发酵产酸的影响袁悦

48820168JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.48No.8Aug．2016doi10．11918/j．issn．0367-6234．2016．08．006100124SCFAspH=1090d．2pHpHpH=7．4±0．2pHpH=10±0．2．pH4．9pH=7．4±0．216．4mg/gVSSCODpH=10±0．2、pH=10±0．2pH=7．4±0．2146．2mg/gVSS、pH、129．8mg/gVSS．SCFAs．pHX703A0367－6234201608－0037－05Effectoffermentedsludgeonshort-chainfattyacidsSCFAsproductionfromexcesssludgeYUANYuePENGYongzhenLIUYeWANGShuyingKeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentＲecoveryEngineeringEngineeringＲesearchCenterofBeijingBeijingUniversityofTechnologyBeijing100124ChinaAbstractToenhancetheproductionefficiencyofshortchainfattyacidsSCFAsinanaerobicsludgefermentationreactorsludgefromanotherstabilizedfermentationreactorover90daysunderpH10wasseededintothereactorstoexamineitseffectonSCFAsproduction．TwosetsofexperimentweredesignedtoinvestigatetheSCFAsproductionwith/withoutseedingsludgeunderpH=7．4±0．2andpH=10±0．2respectively．Experimentalresultsshowedthat1undersamepHconditionthemaximumSCFAsaccumulationwithseedingsludgeadditiveincreasedby4．9mgCOD/gVSSpH=7．4±0．2and16．4mgCOD/gVSSpH=10±0．2respectivelyincomparisonwiththecontrolreactors2withtworeactorswithseedingsludgethemaximumSCFAsaccumulationatpH10±0．2increasedby146．2mgCOD/gVSSascomparedwiththeconditionofpH=7．4±0．23themaximumSCFAsaccumulationatpH10±0．2withoutseedingsludgewas129．8mgCOD/gVSShigherthanthatofpH7．4±0．2withseedingsludge．OverallseedingsludgeimprovedSCFAsproductionefficiencymeanwhileseedingsludgeunderalkalinepHcouldplaysynergeticroleinenhancingtheSCFAsaccumulation．Keywordsfermentedsludgeexcesssludgeanaerobicfermentationshort-chainfattyacidspHvalue2016－02－18515780141988—1949—[e-mail]、60%1．．2－4．、———SCFAs5－9．、、．———SCFAs10－11．3、．12－13．SCFAs．14－15SCFAs8～10d．、．16－18．、．19HIT－01HIT－022．2SCFAs．pH．pH=10、pHSCFAsSCFAs．11．1SBＲ6m3．SBＲ112h0．25h、6h、1h、2h、2．25h、0．5h．．4℃1．1Tab．1Characteristicsofsewagesludgeaftersedimentationmg·L－1TSSVSSTCODSCODSCFAsProteinCarbohydrate7345．8±372．16603．8±333．29732．6±283．527．6±12．721．3±4．75002．8±103．1770．7±53．2TSSVSSTCODCODSCODCODSCFAs．1．21．2．15LSAFＲ．pH10±0．230±2℃SＲT6d833mL833mLSAFＲ．30d90d．1．2．2SAFＲ1200mL2．41pHpH=7．4±0．21#2pHpH=7．4±0．22#3pHpH=10±0．23#4pHpH=10±0．24#．24120r/minθ30±2℃．3min．pH10±0．22mol·L－1NaOH2mol·L－1HCl．1d4000r/min10min0．45μm．1．3DNA、PCＲMiseqSAFＲ90．4000r/min5min．0．0346gDNAFastDNASpinKitforSoilDNA．16SrＲNAV3+V4202．PCＲ20μL5×FastPfuBuffer4μL2．5mmol/LdNTPs2μLForwardPrimer5μmol/L0．4μLＲeversePrimer5μmol/L0．4μLFastPfuPolymerase0．4μLTemplateDNA10ngddH2O20μL．PCＲ95℃2min·83·4895℃30s55℃30s72℃45s2572℃10min4℃．IlluminaMiseqPE300NCBISＲA2308793．2PCＲTab．2SsequenceinformationofprimersforPCＲamplified338FACTCCTACGGGAGGCAGCA806ＲGGACTACHVGGGTWTCTAAT3IlluminaMiseqTab．3StatisticsofsampleSequencesOUTsBases/bpAveragelength/bp2712545911824091435．91OUTOperationaltaxonomicunits0．97．1．4TSS、VSS．TCOD、SCOD5B－3BCOD－Lowry-Folin．SCFAsAgilent7890AFIDAgilentdB-WAXetr30m×0．53mm×0．001mmN220mL·min－1220250℃80℃240℃2μL．SCFAs、、、、1．07、1．51、1．82、1．82、2．04、2．04COD21SCFAs．WTWpH/Oxi340ipH．22．1SAFＲSCFAs1SAFＲ30d60d1721．4±91．2mg/L．IlluminaMiseqphylum102ChloroflexiProteobacteriaBacteroidetesFirmicuteChloroflexiSAFＲ30．1%22．genusIlluminaMiseq4．———Alcaligenes3．47%Anaerolinea3．34%、———Erysipelothrix7．31%Paludibacter4．96%Tissierella3．59%22．SAFＲ．!##!###$##$######%#%&’!’(’&#&&)!)((’*#!+,!#$%+-./012$31SAFＲFig．1SCFAsproductionfromexcesssludgewiththefermentationtime!#$%&’()*+,-./01234542,.456505738’$(9,537:29,./52(9547:29,./521234542,.45650573;=.//?97@59/7:521A237:29,./52)5/@59?,.0B29,./754.,.0C/7,.7:29,./521-D7/7ED.F5!#$%&’()*+,-GH2SAFＲFig．2DistributionofbacterialcommunityinSAFＲ4SAFＲ16srＲNA＞1%Tab．4Phylogeneticclassificationofthe16srＲNAgenesequencesrelativeabundance＞1%atgenuslevelatSAFＲPhylumGenusSAFＲ/%FirmicutesErysipelothrix7．31BacteroidetesPaludibacter4．96FirmicutesTissierella3．59VerrucomicrobiaProsthecobacter3．57ProteobacteriaAlcaligenes3．47ChloroflexiAnaerolinea3．34ChloroflexiLeptolinea1．08ProteobacteriaThauera8．972．2SCFAs3SCFAs．1#1～9d、2#1～9d、3#1～10d、4#1～6d．1#、2#、4#3#．1#、2#pHSCFAs．3#4#17、22·93·8SCFAs．SＲT1#～9d、2#～9d、3#～10d、4#～6d．364#pH=10±0．2187．2mg/gVSS1#、2#、3#36．19d、41．09d170．8mg/gVSS10d．14－153～4d．pH2#1#4．9mg/gVSSP＜0．054#3#16．4mg/gVSS．SCFAs．14－15pH=10±0．2pH=7．4±0．2146．2mg/gVSS．2#3#129．8mg/gVSS．!#!##$#$####$!%&’()*$#$$$!$%$&!#$%+,-./.0$1$2,&’()34*+1!2,,’()34*+1%2,&’()34-$#1&2,,’()34-$#1!+534Fig．3SCFAsproductionfromexcesssludgeESatfourreactors2．3extracellularpolymericsubstancesEPS、SCFAs．EPS70%～80%2、、、23．．4、5．．EPSEPS24．4#3#23、．!#!##$#$##%#%####%$!&’()*%#%%%$%!%&%+,!#$-.%&/$+,’#$-.%&/!+,!#$-.(%#/&+,’#$-.(%#/)*+0,12324%/!0544Fig．4SolubleproteinreleasefromESatfourreactors!#$%&!&’%($)#*!!!!&!’!%!+,!#$-.%&/&+,’#$-.%&/’+,!#$-.(!/%+,’#$-.(!/)*0,12324!/!0554Fig．5SolublecarbohydratereleasefromESatfourreactors、NH4+－N．NH4+－N25．64NH4+－N．4NH4+－N4#＞3#＞2#＞1#．4#．SCFAs．、4#．·04·48!#$%%$#!&’()*%%%%$%#%!%+,!#$-.%&/$+,’#$-.%&/#+,!#$-.(%/!+,’#$-.(%/)*+,-0,12324%/!0564NH4+－NFig．6NH4+－NreleasefromESatfourreactors31．pH4．9mg/gVSSpH16．4mg/gVSS．．2．3．1WEIYuansongVAN-HOUTENＲTBOＲGEＲAＲetal．Minimizationofexcesssludgeproductionforbiologicalwastewate