辅酶Q10的波谱学特征和结构确证牛瑞红

化学工程师Sum154No.7ChemicalEngineer20087:2008-04-01:(1966-),,,,:1002-1124(2008)07-0021-03Q101,2,3(1.,453000;2.,453002;3.,453003):Q10(IR)(UV)(MS)(1H-1HCOSY)(13CNMR,DEPT)(HMQC)(HMBC),1HNMR13CNMR;:Q10;;;;:TQ464.8:ASpectraldataanalysisandidentificationofcoenzymeQ10NIURui-hong1,HAOEr-jun2,ZHOUYong3(1.TeachersFurtherEducationSchoolofXinxiangCounty,Xinxiang453000,China;2.CollegeofChemistryandEnvironmentalScience,HenanNormalUniversity,Xinxiang453002,China;3.DepartmentofChemistryandEnvironmentEngineeringofXinxiangUniversity,Xinxiang453003,China)Abstract:Theinfraredspectrum,ultravioletspectrum,massspectrum,1H-1Hcorrelatedspectroscopy(1H-1HCOSY),(1Hdetected)heteronuclearmultiple-quantumcoherence(HMQC),(1Hdetected)hetero-nuclearmultiple-coherence(HMBC)ofcoenzymeQ10werereportedandinterpreted.Allthe1Hnuclearmagne-ticresonance(NMR)and13CNMRchemicalshiftwereassignedbymeansof1H-1HCOSY,HMQCandHM-BC.Thevibrationsoffunctionalgroupsofthiscompoundwerediscussed.Keywords:coenzymeQ10;nuclearmagneticresonance;assignment;infraredspectrum;ultravioletspec-trumQ10(CoenzymeQ10),,,,[1,2]2-(3,7,11,15,19,23,27,31,35,39--2,6,10,14,18,22,26,30,34,38-)-5,6--3--P-,,,Q10,(IR)(UV)(MS)(1H-1HCOSY)(13CNMR,DEPT)(HMQC)(HMBC),1HNMR13CNMR;Q10[3],11.1PE-2400;PERKIN-ELMERLAMBDA17;BIO-RADFTS-40,KBr();DT-40();D8AD-VANCEX-(BRUKER);Ag-ilentHP1100()1HNMR13CNMRHMQCHMBC(BrukerDPX-400M)D2O,,1HNMR400MHz;13CNMR100MHz1.2Q10牛瑞红等:辅酶Q10的波谱学特征和结构确证20087HPLC,99.5%,(CHO)(%,):C82.02(82.04),H10.50(10.43),O7.48(7.53),22.1Q10Q1059C,904O(CDCl3)11,90,13CNMR18C,Q1059C,Q10H11,1.65710-6,3H,19H1.71710-6,3H,18H1.94510-6,18H,14HH1211H1.99210-6,3H,9H2.05010-6,18H,10H,H1411H3.16310-6,2H,15HH1416H3.95810-6,3H,7H3.97210-6,3H,8H4.91610-6,1H,16H,H151819H5.07510-6,9H,11H,H1013H16.1610-613,9C26.6610-610,9C39.7210-614,9C61.0910-67,8C124.1510-611C,9C135.0810-612C,9C1Q101(CDCl3)/10-61.56727d13-H1.6573s19-H1.7173s18-H1.94518m14-H1.9923s9-H2.05018m10-H3.1632d15-H3.9583s7-H3.9723s8-H4.9161m16-H5.0759m11-H2(CDCl3)/10-6CC11.9099-C16.16113-C17.65118-C25.29115-C25.65919-C26.66910-C39.72214-C61.0917,8-C118.85916-C124.15111-C131.20917-C135.08112-C137.6111-C138.8414-C141.6816-C144.3215-C183.8812-C184.7413-C2.2Q10296529452612cm-1C-H2848cm-1C-O,1649cm-1C=OC=C;C=O,1649cm-11612cm-1,1288126412051153cm-1C-O1447cm-1C-H,1384751cm-111041024cm-1C-O-C,877796cm-1C-H,2220087牛瑞红等:辅酶Q10的波谱学特征和结构确证32.3Q10:(ESI):863,:767640509389258102:2.4Q10,DTA,100~240,DTA48.6TG,243,243,3,(IR)(UV)(MS),Q10[1],,,.Q10[J].,2003,36(4):314.[2],,,.Q10[J].,2004,35(1):73-76.[3].()[M].:,2000.187-199;212-217.Clermont-Ferrand,METabolicEXplorer,,20087(GlycolicAcid)METabolicEXplorer,,,2006,METabolicEXplorerRoquette,,Bio-Hub-Roquette,,,,201050t,23