复合微生态制剂和曝气对梭子蟹养殖废水的净化效果

2013-11-162014-02-272011F300172012F300222013F20001201211620131122012C230031988—。[e-mail]1971—。[e-mail]10．3969/j．issn．1007-9580．2014．02．00312111113160212316021pH、DO、COD、NH3-N、NO－2-N、NO－3-N、、、EMPortunustrituberculatus。3COD57．15%、50．56%、58．85%NH3-N86．96%、80.85%、76．09%NO－2-N54．55%、36．36%、20%57．14%、52．38%、42.86%pHCOD、NH3-N、NO－2-N、NO－3-N。。S949A1007-9580201402-012-06。。、。。1-2。3-4。5。、、、2。、、EM。11．1。、、、、、≥8×1010CFU/g。、、、、、、、≥2×109CFU/mL。EM、、、、、≥6×109CFU/mL。21《》2014412Portunustrituberculatus。1．2200L。52。1D11．0g/m32D22．4mL/m33D3EM0．75mL/m34D4D5。3。pH、DO、COD、NH3-N、NO－2-N、NO－3-N、0h24h、48h、72h、96h、120h、168h、240h。10d25．9～30．1℃。1．3CODNH3-NNO－2-NNO－3-NpHYSIpH100pHDOYSIProODO。1．40．1mLTSA28℃48h30～300CFU。0．1mLTCBS28℃48h。。22．12．1．1pH3pH24h24hpH120h1-a。H2S、CO2pHpH3pH。pHCO2pH。2．1．2DO372h4．5～5．68mg/L1-b。3。72h36mg/L。2．1．3CODCOD1-c。3COD。CODD358．85%D157．15%D250．56%D445．60%D528．77%。、、、COD6COD7。2．1．4NH3-N8-9、10。1-d。324h48h。D186．96%D280．85%D376.09%D458．70%D536．17%。31《》20144122．1．5NO－2-N11。612。D1、D2D348h1-eD4D5120h。48h3D4D5。D154.55%D336.36%D220%D445.45%D5100%。abcdef1pH、DO、COD、NH3-N、NO－2-N、NO－3-NFig．1VariationofpH、DO、COD、NH3-N、NO－2-N、NO－3-Ninexperimentalgroups2．1．6NO－3-N1-f。3120h。120h。41《》2014412DO＜0．5mg/L13-14。3。4．5mg/L。2．22．2．12。24h48h72h1。3。。2Fig．2VariationofHPCinexperimentalgroups2．2．2324h24h48h。＞＞33。2．2．3461．11%345%。33Fig．3VariationofVBCinexperimentalgroups3＜EM＜。4Fig．4Variationoftheratioofvibriotoheterotrophicbacteriainexperimentalgroups33．1、、。16。17。3COD、NH3-N、NO－2-N18、19。3NO－3-N51《》2014412NO－3-N4．5mg/LNO－3-N15。3．28-9。NO－2-NNO－3-N7。pHCOD、NH3-N、NO－2-N、NO－3-N。NO－2-N、NO－3-N。3．3、、。20。21。3。18。324h24h3。3。。43COD、NH3-NNO－2-NNO－3-NpHCOD、NH3-N、NO－2-N、NO－3-N。3。。。。□1．J．2005244790-794．2．J．2013373457-464．3．Lactobacillusspp．LHJ．2008271342-349．4．PS1J．20092114-6．5．J．201022121147-1149．6．J．2009321128-31．7．J．2006131140-145．8．J．2003361．9．pHJ．20126103553-3558．10．4J．201310255-256．11．61《》2014412J．20134035-9．12．3J．201132166-70．13．“”J．2007231173-178．14HOLLOJCZAKOL．NitrateremovalfromdrinkingwaterinafluidizedbedbiologicaldenitrificationbioreactorJ．ActaBiotechnol198775417-423．15．J．2010264275-279．16．J．200928122680-2687．17．J．2010256556-559．18．J．201132147-49．19．J．2012291265-67．20．J．1994182138-142．21．J．200310146-50．DegradationeffectsofcompoundprobioticsandaerationonwastewaterfromtheswimmingcrabPortunustrituberculatusculturepondWANGGengshen12ZHANGTao1XIEJianjun1SHIHui1XUWenjun11MarineFisheriesＲesearchInstituteofZhejiangZhoushan316021China2KeyLaboratoryofMarineCultureandEnhancementofZhejiangProvinceZhoushan316021ChinaAbstractBasedonaeratedinthenightanexperimentonbioremediationofwastewaterfromtheswimmingcrabPortunustrituberculatusculturepondwasmadebythreecomplexprobioticssuchasthelivingmicrobialpurifyingagentsmadeinCangzhouthewaterqualitypurifyingagentsmadeinJiangsuandeffectivemicroorganismsEMmadeinQingdao．ThenthelevelsofpHDOCODNH3-NNO－2-NNO－3-Nheterotrophicbacteriaandvibrioweretesteddynamicallyintheexperimentperiod．Theresultsshowedthatafter10days3compoundprobioticsgroupshadobviouslybioremediationeffectsonthewastewater．TheremovalrateofCODinthewastewaterwas57．15%50．56%58．85%theremovalrateofNH3-Nwas86.96%80．85%76．09%theremovalrateofNO－2-Nwas54．55%36．36%20%andthetotalvibriocountwere57．14%52．38%42．86%lowerthanthecontrolgroup．TheresultsalsoindicatedthatcomparedwiththecontrolgrouppHlevelcouldbeincreasedobviouslyintheaerationgroupsandthewaterqualityindexesofCODNH3-NNO－2-NandNO－3-Nweredecreasedtosomedegree．Onthebasisofthecomprehensiveanalysisthebioremediationeffectofthelivingmicrobialpurifyingagentsgroupisthebestwiththeconditionofaerationinthenight．KeywordsPortunustrituberculatusaquaculturewastewatercompoundprobioticsaerationwaterpurification71《》2014412