固定化反硝化菌对富营养化水体脱氮的试验研究谭佑铭

【】1谭佑铭　罗启芳　王　琳　王　萌(华中科技大学同济医学院环境医学研究所,武汉　430030)　　【　】　　,,。　PVA,,。　40d,,57.5%,,6.7%。,。NO-3-N66.1%()25.3%();(TIN)62.9%()38.8%();COD29.5%()21.9%()。　,。【】　　　　　TheExperimentalStudyontheDenitrificationofanEutrophicWaterBodybyFixedDenitrifyingBacteria　TANYoum-ing,LUOQifang,WANGLin,WANGMengAbstractObject　Tostudythefeasibilityofnitrateremovalbyfixeddinitrifyingbacteriainaneutrophiclakeandsearchforanewresolutionofinsitubioremediationofeutrophiclakes.Methods　HeterotrophicdenitrifyingbacteriaimmobilizedwithPoly-vinylalcohol(PVA)werecastintosimulatingeutrophicwaterenvironmentfordenitrificationanddegradationoforganicpolluta-nts.Results　40dayslater,57.5%oftheammoniaincontrolenvironmentweretransferredintonitratebynitrosobacteriaandni-trobacteria,howeverdenitrificationcapabilityofnativedenitrifyingbacteriawaslimited.Totalinorganicnitrogen(TIN)removalratewasabout6.7%.Fixeddenitrifyingbacteriacouldusepartoftheorganicmaterialsinlakewaterascarbonsourcestoreducenitratesuccessfully.TINremovalratesofhighandlowconcentrationenvironmentwere62.9%and38.8%,respectively.TheCODconsumptionratewere29.5%and21.9%,respectively.Conclusion　Fixeddenitrifyingbacteriamayplayanimportantroleininsituremediationofaneutrophiclake.KeyWords:eutrophication　lake　denitrification　insituremediation　Fixeddenitrifyingbacteria　:1　　(39870664)　　。,、。,。,,,,,,,。1　1.1　1.1.1　:(Polyvinylalcohol,PVA),1750±50,;,,,160;,,;。1.1.2　:。1.1.3　:UASB,PVA-,〔1〕。1.1.4　:,UV-2401,:722,;PIX-Ⅱ,;,,24cm×24cm×60cm。1.2　1.2.1　〔2〕:(DO),-;NO-3-N,;NO-2-N,N-(1-)-;NH+4-N,·65·200322　ChineseJournalofPublicHealthEngineering2003,Vol.2No.2;,-;(CODCr),;,;,。1.2.2　:、,,。1.2.2.1　:,,500ml。66g,30g。、4d,、,。250ml,,C/N=2.0,。6hNO-3-N,,。、4d,、,。1.2.2.2　:,KNO3,NO-3-N45mgL20mg/L,。,500ml,350ml,35℃。,,NO-3-N。1。1　AB(ml)350350COD(mgL)51.951.7NO-3-N(mgL)45.1721.61CODNO-3-N1.152.11pH6.836.87DO(mgL)3.13.2(g)66.066.01.2.3　1.2.3.1　:3(24cm×24cm×60cm)。:①,3,1000g,3;②3,20L,3;③,;④48h,3,CODNO-3-N;⑤3、NO-3-NNO-3-N3;⑥KNO3,,NO-3-N5mgL,NO-3-NNO-3-N。1.2.3.2　:PVA、200g2,100g,N-3-N,。1.2.3.3　:,5d1。、,。NO-x-N、NH+4-N、、CODpH。2　2.1　,A、B60h,NO-3-N,2。2　(h)ANO-3-N(mgL)　　(%)BNO-3-N(mgL)　　(%)045.1724.61440.849.5921.3713.16835.3221.8017.6328.361232.0928.9614.4641.242423.218.625.9175.982821.6352.113.7484.803218.5259.002.0991.513616.1164.331.9492.12489.3979.211.8792.40526.7285.121.7293.01564.3890.301.6893.17603.7191.791.5993.54　　2,60h,ABNO-3-N45.17mgL24.61mg/L3.71mgL1.59mg/L,91.79%93.54%。、,。,、〔3〕。,·66·200322　ChineseJournalofPublicHealthEngineering2003,Vol.2No.2。,COD1,51.9mgL。60h,COD38.7mgL(A)40.8mgL(B),COD25.4%21.6%。,,,NO-3-N。,。2.2　,40d3。3　(n=3)0d　　　　　　　　40d0d　　　　　　　　40d0d　　　　　　　　40dNO-3-N(mgL)2.11±0.513.57±0.655.58±1.021.89±0.512.29±0.471.71±0.39NO-2-N(mgL)0.430.590.640.830.580.72NH+4-N(mgL)3.54±0.681.51±0.353.91±0.771.04±0.333.68±0.971.58±0.41TIN(mgL)6.08±1.205.67±1.0410.13±1.923.76±0.856.55±1.284.01±0.76TN(mgL)6.77±0.976.62±1.0710.65±1.744.33±0.787.28±1.234.87±0.81COD(mgL)33.734.834.624.430.623.9pH6.846.676.766.556.736.68SS(mgL)0.330.360.320.270.320.34DO(mgL)4.283.654.333.174.113.72　　3,,。40d,NH+4-N1.51mgL。,,(t=3.0635,P=0.037546)。6.7%,(t=0.4472,P=0.6778)。、,。53.0%38.8%,(t=5.2545,P=0.006277;t=2.9552,P=0.04175)。COD29.5%21.9%。,,,。,〔4〕,、0.067mg(NO-3-N)/g·m3·d0.032mg(NO-3-N)/g·m3·d。,,、。30℃,,,,9～17℃。,。,5.3mlg,200ml/g,37.7。,,,,。,,1/10。,。2.3　2.3.1　,。,,,。,,,。,,。。PVA,〔1〕。6.8×105/g,1～2〔5〕。PVA,·67·200322　ChineseJournalofPublicHealthEngineering2003,Vol.2No.2,,,。,,。。,。,,。2.3.2　,。,,。。,、,,,〔6〕。,〔7〕。,40d,COD,,。3　3.1　,。3.2　。3.3　。4　〔1〕　,..,2001,18(6):371～373.〔2〕　,...:,1989.246～356.〔3〕　KoelmansA.A,HeijdeA.V,KnijffL.M.Integratedmodelingofeu-trophicationandorganiccontaminantfate&effectinaquaticecosys-tems.areviewWat.Res,2001,35(15):3517～3536.〔4〕　LiujnF.V,BoersP.C,LijklemaL,etal.Nitrogenfluxesandprocessinsandyandmuddysedimentsfromashalloweutrophiclake.Wat.Res,1999,33(1):33～42.〔5〕　,..,2001,29(11):110～112.〔6〕　,..,2002,23(3):29～35.〔7〕　SaraH,MikaelP.Metabolicpropertiesofdenitrifyingbacteriaadaptingtomethanolandethanolinactivatedsludge.Wat.Res,1998,32(1):13～18.(:2002-09-16)《》《》(U.S.CMA)。。:、、、、、、、、、、、、、、、、、、、、、、、、。:A.、、、;、、、、、,。B.(),,300,;2000,2000500(50);30。C.300400,,。D.。E.,、、、,,,。F.,,1～2;。:428,:050071,《》;:、。:0311-70456147045610;:7817145,7045614。[e-mail]。:0461502362。,。·68·200322　ChineseJournalofPublicHealthEngineering2003,Vol.2No.2