过氧化氢对铜绿微囊藻的损伤效应研究

35420154ActaScientiaeCircumstantiaeVol．35No．4Apr．2015No．021210SupportedbytheFundamentalＲesearchFundsforSichuanProvinceNo．0212101989—[e-mail]*[e-mail]1989—[e-mail]*[e-mail]10．13671/j．hjkxxb．2014．0857．2015．J．3541183-1189LiJWangYJGaoP．2015．DamagingeffectsofhydrogenperoxideonMicrocystisaeruginosaJ．ActaScientiaeCircumstantiae3541183-1189李娟1，王应军1，*，高鹏21．6111302．6100722014-06-102014-08-032014-08-03H2O2Microcysticaeruginosa．H2O2．24h、a、T-AOCMDAH2O272hMDAH2O2．H2O250mg·L－1．H2O20253-2468201504-1183-07X172ADamagingeffectsofhydrogenperoxideonMicrocystisaeruginosaLIJuan1WANGYingjun1*GAOPeng21．CollegeofＲesourcesandEnvironmentSichuanAgriculturalUniversityChengdu6111302．SichuanProvincialWaterConservancyＲesearchInstituteChengdu610072Ｒeceived10June2014receivedinrevisedform3August2014accepted3August2014AbstractThisstudyusedindoorculturemethodtoinvestigatethedamagingeffectofhydrogenperoxideH2O2onMicrocystisaeruginosa．ＲesultsshowedthatthetoxiceffectincreasedwithincreasingH2O2concentrations．ThenumberofalgalcellsthecontentofchlorophyllacarotenoidandproteinandT-AOCofMicrocystisaeruginosareducedrapidlyafter24hourswhilethecontentofMDAincreasedsignificantly．MeanwhiletheH2O2contentinculturemediumalsoreducedrapidly．Toxiceffectalsoincreasedgraduallywiththeprocessingtime．Thevalueofeachindexreducedtoaverylowlevelafter72hoursthecontentofMDAincreasedtothemaximumandtheH2O2wasconsumedanddecomposedgraduallyimplyingthatamaximumdamageeffectofalgalcellswasachieved．At50mg·L－1H2O2caneffectivelyremovealgalcellsandexertastrongdamagingeffectonthephysiologicalindexesandanti-oxidizingcapacityofalgal．KeywordshydrogenperoxideMicrocystisaeruginosadamagingeffects1Introduction、、2011．H2O2H2O2Sheldon2005．H2O2ＲOSUzildayetal．2014Mehterovetal．20122012．H2O2Freeseetal．1999Kayetal．1982Heaetal．20132011．H2O235．Microcystisaeruginosa“”．H2O2H2O2H2O2．H2O2H2O2．2Materialsandmethods2．1MicrocystisaeruginosaFACHB912、．、、、、．H2O2．2．220～50mL2/3BG111∶2．25℃2000～2500lx12h/12h3．2BG111∶5～1∶6．2．3Heaetal．201320110、25、50、75100mg·L－10mg·L－1．4500r·min－110min、pH7．5BG115×106·mL－1．H2O2H2O20、25、50、75100mg·L－1．25℃、2500lx3．2．424h、a、、T-AOC、MDAH2O2．2008650nma20062013、T-AOCMDA2005T-AOCMDATBA2010H2O2．、T-AOC、MDAH2O2．Excel2003DPSv7．05．3Ｒesultsandanalysis3．15×106·mL－1H2O23d1．1H2O2H2O2．0mg·L－1p＜0．0572h152%．H2O224h65%48h75mg·L－1100mg·L－1p＞0．0550mg·L－190%72h25mg·L－181%3H2O248h3p＞0．05．481141H2O2Table1EffectsofH2O2onthegrowthandremovalrateofMicrocystisaeruginosaH2O2/mg·L－124h/106·mL－148h/106·mL－172h/106·mL－106．103±0．071Ac－22%9．143±0．156Ab－83%12．613±0．283Aa－152%251．733±0．113Ba65%1．083±0．113Bb78%0．958±0．007Bb81%500．833±0．028Ca83%0．488±0．007Cb90%0．208±0．007Cc96%750．613±0．014Da88%0．253±0．014Db95%0．088±0．007Cc98%1000．453±0．014Ea91%0．148±0．007Db97%0．043±0．000Cc99%±LSDH2O25%H2O25%．3．2aa25．546mg·m－311．445mg·m－3．a10mg·m－3．H2O23da23．2H2O2a．H2O2aH2O2a50、75、100mg·L－1ap＞0．0524ha80%100mg·L－1ap＞0．0575mg·L－148ha50mg·L－1ap＜0．0572ha75mg·L－198%．25mg·L－1a．H2O2a72h．H2O2a．2H2O2aTable2EffectsofH2O2onthecontentsofchlorophyllaofMicrocystisaeruginosaH2O2/mg·L－124hChla/mg·m－348hChla/mg·m－372hChla/mg·m－3029．134±1．822Ac－14%47．832±3．435Ab－87%68．497±3．905Aa－168%2510．729±2．828Ba58%6．387±1．414Bab75%4．598±0．707Bb82%505．123±0．215Ca80%2．084±0．212BCb92%0．631±0．067Bc98%753．832±0．990Ca85%1．277±0．566Cb95%0．392±0．141Bb98%1002．044±1．131Ca92%0．275±0．141Ca99%0．083±0．031Ba100%．3H2O2a．H2O2p＞0．05．H2O224h25mg·L－1H2O2p＜0．0560%50、75、100mg·L－1p＞0．0548h96%．5811353H2O2Table3EffectsofH2O2onthecontentsofcarotenoidsofMicrocystisaeruginosaH2O2/mg·L－124h/mg·m－348h/mg·m－372h/mg·m－3011．852±1．131Aa－4%16．741±2．828Aa－46%15．047±0．495Aa－31%254．571±0．283Ba60%2．108±0．057Bb82%0．874±0．085Bc92%501．947±0．283Ca83%0．479±0．184Bb96%0．069±0．011Cb99%751．341±0．141Ca88%0．204±0．042Bb98%0．027±0．011Cb100%1000．613±0．156Ca95%0．036±0．007Bb100%0．004±0．001Cb100%3．3H2O21．128．654×10－7μg·cells－172h0mg·L－1H2O2p＜0．05H2O2p＜0．05H2O2．1H2O2Fig．1EffectsofH2O2onthecontentsofproteinofMicrocystisaeruginosa3．4H2O272h2．T-AOC．0mg·L－125mg·L－1H2O2T-AOCp＞0．053T-AOC25mg·L－1H2O2T-AOC24h72h5%．50、75100mg·L－13H2O2T-AOCT-AOC24h3T-AOCp＜0．053．105、2．5672．104U37℃107OD0．01U17%、34%44%48h3T-AOCp＞0．0572hT-AOC86%、97%98%．H2O2T-AOCH2O2．2H2O2T-AOCFig．2EffectsofH2O2onthetotalantioxidantcapacityT-AOCofMicrocystisaeruginosa3．5H2O272hMDA3．68114MDA．3MDA72hp＜0．05．H2O2MDAp＜0．05H2O2MDA．72h25、50、75、100mg·L－1H2O2MDA0．936×107nmol·cells－175%、92%、163%270%．H2O23H2O2MDAFig．3EffectsofH2O2onthecontentsofmalondialdehydeMDAofMicrocystisaeruginosaMDA．3．6H2O2H2O2H2O272h4．H2O2H2O2H2O2．4H2O2H2O272hH2O234%0．343mg·L－1．H2O2H2O2p＜0．05．H2O2H2O2H2O2H2O224h25mg·L－1H2O281%50、75、100mg·L－1H2O269%、65%62%72hH2O290%．50mg·L－1H2O20．975mg·L－175mg·L－1100mg·L－1p＜0．0525mg·L－1p＞0．05H2O298%．100mg·L－1H2O272hH2O290%H2O210．014mg·L－1．4H2O2Table4ContentsofH2O2inmediumH2O2/mg·L－10hH2O2/mg·L－124hH2O2/mg·L－148hH2O2/mg·L－172hH2O2/mg·L－100．000±0．000Ec0．103±0．028Ebc10%0．214±0．074Eab21%0．343±0．074Ca34%2525．000±1．078Da4．752±0．707Db－81%3．076±0．170Dc－88%0．412±0．021Cd－98%5050．000±1．565