海洋厌氧氨氧化研究进展魏海峰

37anaerobicammoniumoxidationANAMMOXNH4+NO2-[1]。。1941NO2-NH4+N2[2]。1977BrodaNO3-、CO2NO2-NH4+N2NO2-[3]。1995Delft《》2013-12-122014-03-105100804521377014IRT081[e-mail]*[e-mail]。EnvironmentalScience&Technology37920149Vol.37No.9Sept.2014魏海峰1，2，周集体1，乔森1*，刘远21.1160242.116023、、。3———、C3。15NDGGE、16SrRNA-PCRFISH。、、。。X834Adoi10.3969/j.issn.1003-6504.2014.04.0151003-6504(2014)04-0070-06ResearchProgressinMarineAnaerobicAmmoniumOxidationWEIHaifeng12ZHOUJiti1QIAOSen1*LIUYuan21.SchoolofEnvironmentDalianUniversityofTechnologyDalian116024China2.SchoolofOceanTechnologyandEnvironmentDalianOceanUniversityDalian116023ChinaAbstractThispaperreviewedtheresearchesathomeandabroadaboutanaerobicammoniumoxidationANAMMOXwhichisregardedasapotentialpathwayforN2formationandNremovalincoastalmarinesedimentsandinanoxicwatercolumnsofoceans.OfachievementmadeinANAMMOXresearchareawasthefindingofANAMMOXbacteriathathasamembrane-boundcompartmentinthecellknownastheanammoxosomethatcontainsladderanelipidsandcytochrome-C.ThestoichiometricstudiesofANAMMOXwerebasedontheexperimentsusinga15Nisotopelabeling16SrRNA-PCRDGGEandFISHtechniques.ItwaspointedoutthatANAMMOXresearchinChina’scoastalseashasrarelybeencarriedoutyethowevertheanoxicstateinsedimentsandwatercolumnoftheseashasbeendetectedduetheincreasedpollutionsothedistributiondiversityandabundanceofANAMMOXbacteriainmarineenvironmentoughttobestudied.KeywordsANAMMOXbacteriaANAMMOXnitrogencyclebiologicalnitrogenremoval9[4]。VandeGraaf15N[5]。。。。。11.1海洋厌氧氨氧化细菌研究1.1.1。Anammox、[6]。。KuypersCandidatusscalindua[7]。5Candidatusbrocadia、Candidatuskuenenia、Candidatusscalindua、CandidatusanammoxoglobusCandidatusjetteniaCandidatusscalindua[8]。2[9]。。1.1.2。1S。Anammoxosome。。Anammoxosome[10]。2———Ladderanelipids[11]。N2H4。JaeschkeIrishCeltic[12]。3C468nm。CCC。1.1.311d。Strouspercoll99.6%[13]。37℃-1℃Skagerrak4～6℃[14]。。、。。1.1.41977Broda[3]2NH4+N2。1994Mulder[1]DelftNH4+NH4+NO3-N2。NH4+、NO3-3NO3-+5NH4+=4N2+9H2O+2H+ΔG0=-297kJ/molBroda。1997vandeGraaf15NNO2-37NO3-ANAMMOXNO2-+NH4+=N2+2H2OΔG0=-358kJ/mol15NAnammoxNO2-NO3-。NO2-N2NO2-。NO2-NO3-[5]。NH4+NO2-NO3-NH4+∶NO2-∶NO3-=1∶1.32∶0.26。13CAnammoxA。1.2国外海洋厌氧氨氧化研究1995。2000Thamdrup[15]。2002Thamdrup24%～67%N2[16]。DalsgaardGolfoDulce200m15NN2N219%～35%[17]。Engstrom、7N2N24%～79%[18]。Trimmer[14]。WoebkenNamibian[19]。Jensen、[20]。RichChesapeakeChoptankPatuxent15%～20%0～10%NH4+NO3-[21]。JensenMariager[22]。、、。[23][24]、[8][25-26]。。DGGE[27]。16SrRNA[28]。Strous[29]。1.3海洋厌氧氨氧化的研究方法、15N[26]。O2NO3-、CO2、NO2-。315NH4+15NH4++14NO3-15NO3-。N2N215N。。、。16SrRNA-PCR、16SrRNA-PCR-DGGE、16SrRNA-Realtime-PCR、16SrRNA16S-23SrDNAFISH、、[27]。。9。。1.4厌氧氨氧化的应用研究1.4.1NO3-、NO2-NH4+[20]。。Anammox。87%1000m3800m。DalsgaardN21/3～2/3[17]。Ward30%～50%N2[6]。Ward[9]。。。。。1.4.2C/N、。C/N。。。Nakajima31a[30]。Vossenberg2cm1090%[31]。Takehiko1.6kg/m3·d[32]。Yasunori150～250d80%0.4kg/m3·d[33]。NH4+NO2-。6。1.4.31。Anammox、、anammox。anammox。2。AnammoxAnammoxScalinduaAnammoxBrocadiaKueneniaAnammox[34]。2。。。。、37、、。1、。2。3C/N。。[][1]MulderAVandeGraafAARobertsonLAetal.Anaer－obicammoniumoxidationdiscoveredinadenitrifyingflu－idizedbedreactor[J].FEMSMicrobiologicalEcology199516177-184.[2]DalsgaardTThamdrupBDonaldEetal.Anaerobicam－moniumoxidationANAMMOXinthemarineenvironment[J].ResearchinMicrobiology2005156457-464.[3]BrodaE.Twokindsoflithotorophmissinginnature[J].Zeis－chriftfurAllgemeineMikrobiologie1977176491-493.[4]VandeGraafAABruijnPDRobertsonLAetal.Au－totrophicgrowthofanaerobicammonium-oxidizingmicro-organismsinafluidizedbedreactor[J].Microbiology19961422187-2196.[5]VandeGraafAAdeBruijnPRobertsonLAetal.MetabolicpathwayofanaerobicammoniumoxidationonthebasisofN-15studiesinafluidizedbedreactor[J].Microbi－ology-UK19971432415-2421.[6]WardBB.Significanceofanaerobicammoniumoxidationintheocean[J].TrendsinMicrobiology2003119408-410.[7]KuypersMMMSliekersAOLavikGetal.AnaerobicammoniumoxidationbyANAMMOXbacteriaintheBlackSea[J].Nature2003422608-611.[8].[J].2009493281-286.HongYiguoLiMengGuJidong.Bacterialanaerobicam－moniaoxidationANAMMOXinthemarinenitrogencycle[J].ActaMicrobiologicaSinica,2009493281-286.inChinese[9]WardBBDevolAHRichJJetal.DenitrificationasthedominantnitrogenlossprocessintheArabianSea[J].Nature200946178-81.[10]KartaBRattrayJVanNiftrikLAetal.Candidatus“Anammoxoglobuspropionicus”anewpropionateoxidizingspeciesofanaerobicammoniumoxidizingbacteria[J].Sys－tematicandAppliedMicrobiology20073039-49.[11]SinningheDJSRijpstraWIGeenevasenJAetal.Struc－turalidentificationofladderaneandothermembranelipidsofplanctomycetescapableofanaerobicammoniumoxidationANAMMOX[J].FEBSJournal2005272164270-4283.[12]JaeschkeARooksCTrimmerMetal.ComparisonofladderanephospholipidandcorelipidsasindicatorsforanaerobicammoniumoxidationANAMMOXinmarinesediments[J].GeochimicaetCosmochimicaActa2009732077-2088.[13]StrousMFuerstJAKramerEHetal.Missinglithotrophidentifiedasnewplanctomycete[J].Nature1999400446-449.[14]TrimmerMNichollsJCMorleyNetal.Biphasicbehav－iorofanammoxregulatedbynitriteandnitrateinanestuarinesediment[J].AppliedEnvironmentalandMicrobiol