好氧反硝化细菌的筛选及其在鸡粪发酵氮素转化中的作用王立群

２００8,２7(6):2494-2498ＪｏｕｒｎａｌｏｆＡｇｒｏ-ＥｎｖｉｒｏｎｍｅｎｔＳｃｉｅｎｃｅ、。、。3SF35-1、JF45-2JF35-1、。3。SF35-1JF45-2。。X172A1672-2043(2008)06-2494-052008-03-07C0203051956—。[e-mail]150030ScreeningofAerobicDenitrifiersandTheirFunctioninNitrogenTransformationofAerobicCompostingofChickenManureWANGLi-qun,XIAOWei-wei,CAOLi-qun,YUQi-lin,SUNWen,GUWen-jieCollegeofLifeSciences,NortheastAgriculturalUniversity,Haerbin150030,ChinaAbstractAerobicdenitrifyingbacteriawereisolatedatdifferenttemperaturestagesincompostingofchickenfeceswithstrawasconditioner.Thedominantstrainswerescreenedbymeasuringde-nitrificationability.Thenthedominantstrainswerere-culturedintothecompostingofchickenfecesandthecontentsofammonianitrogen,nitratenitrogenandtotalnitrogenweremeasured.Theresultswereasfollowstherewereaerobicde-nitrifiersinthecompostingofchickenfeces.Threedominantstrains,namedSF35-1,SF45-2andJF35-1,werescreenedbyde－terminingactivityofnitrogenremoval,andidentifiedasBacillussp.,Derxiasp.andKlebsiellasp.respectively.Thethreestrainswerealliso－latedfrommid-temperaturestage.Itshowedthatde-nitrificationwasaffectedremarkablybytemperatureandwasstrongerinmid-tempera－turestagethanotherstages.TheSF35-1Bacillussp.andJF45-2Derxiasp.alsohadtheabilityofnitrification,whichwasweakerthantheabilityofde-nitrification.Thedominantaerobicdenitrifyingbacteriainoculatedintocompostresultedinreductionofnitratenitrogenandtotalnitrogencontents,andsuggestedthatdenitrifyingbacteriahadde-nitrificationinchickenfecesfermentation.Itwouldreducethenitro－genlossandairpollutionbyeffectivelyregulatingtheactivityofthisgroupofbacteria.Keywordsaerobicdenitrifiers;compostingofchickenmanure;nitrate。。pHN2、N2O[1-2]。[3][4]。２76。Patureau[5]、[6][7]、、。。。11.11.1.1[8]。1.1.2∶∶1∶1.6∶2.1C/N2565％。1.1.3、FOSS2100、ZHWY211B。1.21.2.18mg·L-165℃。10℃25、3545℃5565℃4535℃7。1.2.2。1.2.34%50mL250mL3、130r·min-1。[9]、α-[10]。1.2.41∶12%。、、。[11]-[11]-[11]。1.3《》8[12]。、、、、、。22.11296h1。SF35-1、JF45-2JF35-182mg·L-1P＜0.01。SF25-3、JF55-1、JF35-2。35、45℃55℃SF55-132.5mg·L-165℃。。2.22SF35-10～24h2495２００811105.0mg·L-172h96h33.5%48h。JF45-20～24hSF35-1160.4mg·L-148h96h44.4%0～48h。JF35-10～48h96h30.6%。3。SF35-1、JF45-224h①24h②。RobertsonScholten[13-14]。。2.3316d0～6d12d0.15g·kg-10.24g·kg-1。。4。。。3。56d0.11%。。23Figure2Nitrogenremovalofthethreedominantdenitrifyingbacteria2496２7613Table1ResultsofphysiologicalandbiochemistryexperimentsofthethreedominantdenitrifyingbacteriaSF35-1JF45-2JF35-1SF35-1JF45-2JF35-1M.R.++-+--V.P.+-++--0.5%NaCl+++--+2%NaCl+++---7%NaCl+-+---10%NaCl-----+++++++---+++--+--+---+-+-----+-+---+-----+。2.4、1《》83SF35-1、JF45-2、JF35-1Bacillus、DerxiaKlebsiella。31、3SF35-1、JF45-2JF35-1Bacillus、DerxiaKlebsiella。SF35-1JF45-2。2。3。。[1]TiquiaSM,TamNFY.Fateofnitrogenduringcompostingofchickenlitter[J].EnvironmentalPollution,2000,110535-541.[2]EklindY,KirchmannH.CompostingandstorageoforganichouseholdwastewithdifferentlitteramendmentsNitrogenturnoverandloss－es[J].BioresourceTechnology,2000,74125-133.[3]OslisoA,LewandowskiZ.Inhibitionofbyselectedorganiccompounds[J].WaterResearch,1985,194230-4236.[4],,.[J].,2005,241165-168.ZHANGYu-qin,LIUKai-qi,WANGGe.Screeningofdenitrifyingbac－teriaanddeterminationofthecultureconditions[J].JournalofAgro-en－vironmentalScience,2005,241165-168.[5]PatureauD,BernetN,etal.Effectofdissolvedoxygenandcarbonnitro－genloadsondenitrificationbyanaerobicconsortium[J].AppliedMicro－biologyandBiotechnology,2000,54535-542.[6],,,.[J].,2007,111-13.ZHAIQian,WANGPing,LIXiu-ting,etal.Selection,enrichmentandidentificationofaerobicdenitrifiersinactivatedsludgesystem[J].Envi－ronmentalScience&Technology,2007,111-13.[7]ZhouLi-xiang,HuangFeng-yuan,WangShi-mei.Isolationofaerobicdenitrifiersandtheirrolesinsoilnitrogentransformation[J].ActaPedo－logicaSinica,2006,433430-435.[8],,.[J].,2005,20280-83.MAFang,WANGHong-yu,ZHOUDan-dan.Selectionandenrichment“+”“-”。2497２００811ofaerobicdenitrifierinactivatedsludgesystem[J].JournalofHunanScienceandTechnologyUniversity,2005,20280-83.[9],.[J].,2002,226107-110.BAILan,DUJi-yu.Determinationofnitratenitrogeninvegetable[J].Agriculture&Technology,2002,226107-110.[10]..[D].2004.CAOXi-tao.Nitrogenlossesandinoculationwithheterotrophicnitrifi－erduringthecompostoflivestockandpoultrymanure.MasteralDis－sertationofNanjingUniversityofAgriculture[D].NanjingNanjingUni－versityofAgriculture2004.[11],.[M].,2000.XUJing-an,PANZhen-yu.Analysisandmeasurementmethods[M].BeijingChemicalIndustryPress,2000.[12].[M].,1978.InstituteoftheChineseAcademyofSciencesMicrobialBacteriaClas－sificationunit.Themethodsfornormalbacteria[M].BeijingSci－encePress,1978.[13]RobertsonLA,KuenenJG.Combinedheterotrophicnitrificationandaer－obicdenitrificationinthiosphaerapantotrophaandotherbacterial[J].An.VanLeeuwenhoek,1990,56289-299.[14],,.[J].,2001,27122-24.LICong-na,LVXi-wu,etal.Studyonnitrogenremovalbysimultane－ousnitrificationanddenitrification[J].Water&WastewaterEngineer－ing,2001,27122-24.《》、。、、500《》。,《》CA《》AJ《、、、》7。、。、、、、。《》1621640.00480.00。6－64。。3001913102223674336,[e-mail]《》
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