您好,欢迎访问三七文档
当前位置:首页 > 行业资料 > 其它行业文档 > DGGE技术在环境微生物多样性研究中的应用
©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1,410004;2,410004):(denaturinggradientgelelectrophresis,DGGE),PCR2DGGE,:PCR2DGGEApplicationinResearchonMicrobialDiversityofEnvironmentbyDGGETechniqueZhangZhenni1,2WuXiaofu1ChenYonghua1,2ShiHui1(1InstituteofEnvironmentScienceandEngineering,CentralSouthUniversityofForestryandTechnology,Changsha410004;2InstituteofBiologyEnvironmentScienceandTechnology,CentralSouthUniversityofForestryandTechnology,Changsha410004)Abstract:Microorganismplaysanimportantroleinwastewatertreatment1Accountedforbyitshighlevelsofreliabilityandrepro2ducibilityaswellasitsconvenienceinoperation,thedenaturinggradientgelelectrophoresis(DGGE)hasbeenwidelyusedforcultiva2tionandidentificationofmicrobialcommunitiesinthefieldsofenvironmentalscienceswithfocusonpollutioncontrol1TheprincipleoftheDGGEtechniqueandtheconcernedkeyfactorsinitsapplicationforanalysisofmicrobialcommunitiesweredescribedinthispa2per1Incomparison,thepotentialapplicationareasoftheDGGEtechniqueanditslimitationswerealsodiscussed1Keywords:PCR2DGGEMicroorganismDiversity:2009207220:(2008ZX072122001),(20072DFA91420),(2008RS4027),(2008001A),,(2006180):(19852),,,:;E2mail:zhangzhenni_2003@1631com:(19532),,,:;E2mail:wuxiaofu530911@vip11631com,,[1,2],,1985Pace[3],1993Muyzer[4]DGGE,DGGEDNA,,DNA,,Muyzer[4]1%DGGE,011%10%,DGGE,1DGGEDGGEDNA,PCR©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(DNA),,DNA(1)1DGGE2DGGE211DNADNADGGE[5],DNA,DNADNA[6],,,DNA[7],,DNA[8],1,3,DNADNA(PEG)DNA,DNAPorteous[12],Miller[13]SDSDNA1DNA,DNA,SDS2[11]SDS2SDSDNA;SDSDNA,DNA[9]SDSDNA,[10]CTAB2SDSDNA,,[10],DNANycodenz[15]Blending[16]DNA,Nycodenz,,20%50%[14],,;Blending,,212PCRPCRPCR2DGGE16SrDNAPCR16SrDNA:(1)16SrDNA1540,,[17];(2)16SrDNA;(3)16SrDNA,,[18,19];(4)16SrDNA,GenBankRibosomalDatabaseProject(RDP),16SrDNA338f/518r(V3)341f/926r(V3V5)968f/1401r(V6V8)Yu94©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[20]V3V3V5,V3PCR2DGGE,V3V5PCR3,TaqDNADNAPCR[21,22],DNA,PCR,,PCR:941min;501min721min,33,725min:90962,,90,944060,5,4550,25303540,35,,PCR213,,DGGE,[23]DGGE,,1DNADGGE,200bp,8%DGGE,Tm(),[24]30%(Tm10),DNA5065,Tm,60[25],,MuyerSmalla[26],Sigler[23],DGGE,,3,1000V,,DGGE,(EBSYBRGreenI),SYBRGreen,;,[27]3DGGE311DGGE[28]PCR2DGGE,,,,,[29]PCR2DGGE,,16SrDNAPCR626bp,16SrDNAV3V5,5[30]PCR2DGGE,SBR,SBR,,05©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[31]PCR2DGGE,,,,,312DGGE[32]PCR2DGGE,DGGE16SrRNAV3DNA,DNA[33]DGGE,16SrDNAV3,[34]PCR2DGGE,,,Muller[35]PCR2DGGEHg,Hg,Hg,,313DGGE[36]PCR2DGGE,,,,[37]PCR2DGGE,,[38]PCR2DGGE,[39]PCR2DGGE(SBBR)SBBR,,,4DGGEDGGE:(1)(500bp),,(2)DGGE,Sehiguchi[40]DGGE,DNA,DNA(3)DGGErDNA,l%DGGEVal2laeys[41]DGGEDNA(4)16SrDNA,[42](5)DGGE,,DGGEDGGE,:(1)DNAPCR;(2)DGGE(3)DGGE[37],,,,PCRDGGEDGGE/TGGE,;PCR15©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(4),,DGGE1HoppHG,EmerickLC,GockeK1AppliedandEnvironmentalMicro2bial,1998,54:784790.2MatinCD,MoshiriGA1WaterScienceandTechnoly,1994,29(4):267272.3PaceNR.Science,1997,276:734740.4MuyzerG,DeWaalEC,UitterlindenAG1AppliedEnvironmentMi2crobiology,1993,59:695700.5OgramA,SaylerGS,BarkayT1JournalofMicrobiologicalMethods,1987,7:5766.6LauragGL,JamesR,DanaJ,etal1AppliedEnvironmentMicrobiolo2gy,1995,59:11411143.7TsaiYL,OlsonBH1AppliedEnvironmentMicrobiology,1991,57:10701074.8JacobsenCS,RasmussenOF1AppliedEnvironmentMicrobiology,1992,58:24582462.9,,,.,2005,13(3):377381.10,,.,2008,28(3):4144.11,,1,2003,43(2):276282.12PorteousLA,SeidlerR,WatrudLS1MolecularEcology,1997,6:787791.13MillerDN,BryantJE,MadsenEL,etal.AppliedEnvironmentalMi2crobiology,1999,65:47154724.14BakkenLR,LindahlV,NucleicAcidsintheEnvironment:MethodsandApplications1Berlin:Springer2VerlagBerlin,1995:927.15BerryAE,ChiocchiniC,SelbyT1FEMSMicrobiologyLetters,2003,223:1520.16GaborEM,CriesEJ,JanssenDB1FEMSMicrobiologyEcology,2003,44(2):153163.17,.:,1993.18,,,1:,2001,767763.19,,,.,2004,31(6):9094.20YuZ,MorrisonM.AppliedandEnvironmentalMicrobiology,2004,70:48004806.21,,.,2008,24(4):3336.22,,.(),2008,39(4):432436.23SiglerWV,MiniaciC,ZeyerJ1JournalofMicrobiogicalMethods,2004,57:1722.24,,1,2004,44(6):845848.25,,.,2006,27(7):14241428.26MuyerG,SmallaK1AntonievanLeeuwenhoek,1998,73:127141.27,,.,2008,33(10):3943.28,,.,2005,25(4):842847.29,,,.,2006,26(11):36113615.30,.12006,22152219.31,.(),2007,40(3):9296.32,,,.,2003,23(8):15701575.33,1,2008,14(2):253257.34TengY,LuoYM,ZhaoXW,etal1ActaPedologicaSinica,2004,41(3):343347.35MullerAK,WestergaardK,ChristensenS,etal1FEMSMicrobiologyEcology,2001,36:1119.36,,,.,2008,41(3):356361.37,,.,2007,27(3):386390.38,,.,2008,36(21):89568959.39,,.,2007,28(5):10951101.40SekiguchiH,TomiokaN,NajagaraT,etal1BiotechnologyLetters,2001,23(15):12051208.41VallaeysT,ToppE,MuyzerG,etal1FEMSMi
本文标题:DGGE技术在环境微生物多样性研究中的应用
链接地址:https://www.777doc.com/doc-6605979 .html