一种快捷小鼠心肌梗死模型的建立

··1007-3949201220-03-0212-05100029。40C57/B6251534。28。2880%100%。283.52±0.10mm4.65±0.08mmP＜0.00012.60±0.19mm4.36±0.13mmP＜0.000166.70%±1.41%29.70%±1.64%P＜0.000135.90%±1.01%14.20%±0.80%P＜0.0001。45.10%±1.53%HE、、Masson。。R5AEstablishmentofMurineModelofMyocardialInfarctionbyaRapidandEfficientProcedureSHIHong-TaoWANGYingZHANGJun-MengWANGLu-YaandDUJieKeyLaboratoryofRemodeling-RelatedCardiovascularDiseasesCapitalMedicalUniversityMinistryofEducationandBeijingInstituteofHeartLungandBloodVesselDiseasesBeijingAnzhenHospitalAffiliatedtotheCapitalMedicalUniversi-tyBeijing100029ChinaKEYWORDSMouseRapidandEfficientAnimalModelMyocardialInfarctionABSTRACTAimTointroducearapidandefficientmodelofmyocardialinfarctionMIwithouttherequirementofventilationinthemouseandcomprehensivelydescribeitsdetails．Methods40MaleBABL/C6miceweredividedinto2groupsnewmethodMI15andnewmethodSham25．Inthenewmethodheartsoftotal40miceweremanu-allyexposedthroughasmallincisionoftheleftfourthintercostalspacewithoutintubation．IngroupofMItheleftanteri-ordescendingcoronaryarterywasligated．HeartsfromgroupofShamwerewithoutligation．ThisnovelMIprocedureisrapidwithanaverageproceduretimeof60±15seconds．WecomparedthedifferencebetweenMIandShampost28dayswithanalysisofechocardiographymorphologyandpathology．ResultsSurvivalrateofgroupMIwas80%andgroupShamwas100%28daysaftersurgerywithourmethod．Theechocardiographyshowedthattheheartfunctionofthemicewith28-daypost-MIwasmarkedlyimpaired．TheLVIDdandLVIDsweresignificantlyincreasedfrom3.52±0.10mmand2.60±0.19mmto4.65±0.08mmand4.36±0.13mmP＜0.0001whileLVEFandLVFSweredecreasedsig-nificantly66.70%±1.41%and35.90%±1.01%vs29.70%±1.64%and14.20%±0.80%P＜0.0001respective-ly．Anatomicalanalysisofheartswith28-daypost-MIshowedenlargedleftventriclesandthi[e-mail]。[e-mail]。[e-mail]。212ISSN1007-3949ChinJArteriosclerVol20No32012Theinfarctsizereachedto45.10%±1.53%．HematoxylineosinHEImmunohistochemistryIHCandmassontri-chromeMassonstainingconfirmedplentyofmacrophagesinfiltrationandfibrosisformation．ConclusionThisnewrapidmethodofMIinmicerepresentsamoreefficientmodelofmyocardialischemicinjury．、。。、。1。、。2。11.1SPFBALB/C40925±5g。3R。1.2、3-06-0polypropyleneVisualSonicsVevo770XGI-8GasAnesthesiaSystemNikonMODELC-F12302%75%。1.3chamber。。。1.41。1。2。3～4341.5cm。5～63-0proline。7～8。9～103-4。。11～122mm6-0proline1mm。13～14。15～16。。1。3～512。1.5281%。MLVinternaldiametersofdiastoleLVIDdLVinternaldiametersofsystoleLVIDsLVe-jectionfractionLVEFLVfrac-tionalshorteningLVFS。1.6281%80mg/kg。。。5μm。TTCHE、MassonMac-2。1.7x±sSPSS16.0tP＜0.05。312CN43-1262/R20122031．Figure1．Capturesofprocedureofmurinemyocardialinfarctionmodel2．。。Figure2．Demonstrationoftheleftanteriordescendingcor-onaryarteryandtheligationsite22.17.5%11/4021/4031/40。12。42080%100%。2.24P＜0.0001P＜0.0001。31。1．Table1．OutcomesofcardiacfunctioninMIgroupcom-paredwithshamgroupn=15n=20LVIDdmm3.52±0.104.65±0.08aLVIDsmm2.60±0.194.36±0.13aLVEF66.70%±1.41%29.70%±1.64%aLVFS35.90%±1.01%14.20%±0.80%aaP＜0.0001。2.328。TTC。45.10%±1.53%4。HE28412ISSN1007-3949ChinJArteriosclerVol20No32012Mac-25。4Masson5。3．Figure3．Monitorofcardiacfunctionwithechocardiography4．ABTTCC。aP＜0.0001。Figure4．MorphologicchangesofheartafterMIoperation5．4AHEBMassonC。Figure5．ComparisonofsectionedtissuesstainedwithHEMassonandMac-23。、、。、、、、3。。、、、、、Ameriod。512CN43-1262/R20122034-7。8。25±5min2。。。。。＜1.5min。20。。1500。。。。3＜1.5min。。。。。。。。、。。。、1cm1～2cm。。。30s。。。。、。。。。1500。。。。1GondoYFukumuraRMurataTetal．Next-generationgenetar-getinginthemouseforfunctionalgenomicsJ．BMBRep2009426315-323．2GaoErheLeiYHShangXYetal．AnovelandefficientmodelofcoronaryarteryligationandmyocardialinfarctioninthemouseJ．CircRespublishedonline2010107121445-453．3．J．2010183196-198．4AhnDChengLMoonCetal．Inductionofmyocardialinfarctsofapredictablesizeandlocationbybranchpatternprobability-assistedcoronaryligationinC57BL/6miceJ．AmJPhysiolHeartCircPhysiol2004286H1201-207．5KlockeRTianWKuhlmannMTetal．SurgicalanimalmodelsofheartfailurerelatedtocoronaryheartdiseaseJ．CardiovascRes20077429-38．6vandenBosEJMeesBMdeWaardMCetal．Anovelmodelofcryoinjury-inducedmyocardialinfarctioninthemouseacomparisonwithcoronaryarteryligationJ．AmJPhysiolHeartCircPhysiol2005289H1291-300．7MostPSeifertHGaoEetal．CardiacS100A1proteinlevelsde-terminecontractileperformanceandpropensitytowardheartfailureaf-termyocardialinfarctionJ．Circulation20061141258-268．8．J．200616141-44．612ISSN1007-3949ChinJArteriosclerVol20No32012