铁皮石斛降血糖作用及其机制的研究

13,1,2,2(1.,310031;2.,310012)[]::,,(STZ2DM),HRP2SPA:,STZ2DM,,:STZ2DM,,[];;;;[]R285.5[]A[]100125302(2004)0220160205,,,,[1,2],,[3],,11.1,1.8g,gkg-1(STZ),89H0604,Sigma,981127,,,045C,Dako,1500,G22654,Sigma[]2002210211[]3,Tel:[phone],Fax:[phone]E2mail:[e-mail](SRP2SPA),BA1080,,15001.2NIH,,1824g;SD,,180240g22.130,3,20mLkg-10.25,0.5gkg-1,ig1,3d,2h,,,0.25,0.5gkg-1,ig(1)1(xs,n=10)/gkg-1d-1/mmolL-1/103UL-1205.21.024.25.20.254.90.91)24.55.01)0.55.00.71)23.05.11):1)P0.052.240,4,20.5,1.0gkg-1,ig,20mLkg-1,06129220042ChinaJournalofChineseMateriaMedicaVol.29,Issue2February,2004ig,1,3d70min,0.2mgkg-1,sc,50min,,sc,(P0.001)0.5,1.0gkg-1(P0.001),1.0gkg-1(P0.01,2)2(xs,n=10)/gkg-1d-1/mmolL-1/mgg-14.90.62)9.11.32)12.32.41.81.10.57.11.02)2.71.81.06.70.92)4.31.81):1)P0.01,2)P0.0012.3STZ2DM[4]16h,,ipSTZ70mgkg-1,72h,16h,,16mmolL-13,620.125,0.5gkg-1,ig;1,,2h,,,0.125,0.25gkg-1,ig,STZ(P0.001);0.25gkg-1ig(P0.05),0.125,0.25gkg-1,ig(P0.05P0.01),(3)3STZ2DM(xs,n=6)/gkg-1/mmolL-1/103UL-1/103pgL-15.80.23)24.46.53)91133)STZ19.71.714.02.9140200.1256.90.63)17.63.6102221)0.255.60.33)20.65.11)98192):STZ1)P0.05,2)P0.01,3)P0.0012.4STZ2DM,2.4.1STZ2DM,12,2,0.25gkg-110mLkg-1,ig,2,7d,51hipSTZ100mgkg-1,STZ3d2,,,4%,,,HRP2SPA[5],,Tris2HCl,,,ipSTZ100mgkg-13d,(23.82.2)mmolL-1;(14.97.5)mmolL-1,(P0.05),,,(1),STZ3d,1,2,4(2)2,8.9,5.2mmolL-1,,,(3)12400400,16129220042ChinaJournalofChineseMateriaMedicaVol.29,Issue2February,2004,,,(5),,,,(6)2,,(7)3440020056400400784004002.4.2STZ2DM,24,31,2ipSTZ100mgkg-1,3d0.25gkg-1,ig,10mLkg-1,ig,2,41,2,4,,2,,1,2,4(P0.001)24,,2(P0.05),4,(4)40.25gkg-1ig2STZ2DM(xs)/mmolL-11245.30.82)(8)6.40.52)(6)5.50.72)(4)STZ21.13.0(8)28.72.5(6)28.82.5(3)21.01.9(8)21.35.51)(6)16.68.9(3):;STZ1)P0.05,2)P0.001,1,,,;,24,,,(4);,(8)33.1,STZ2DM,,,,,,,,3.2,STZ2DM,ip100mgkg-1STZ,26129220042ChinaJournalofChineseMateriaMedicaVol.29,Issue2February,2004,,,STZ4,,[5],,,,STZ,STZ,,3.3,,,,,,,3.4,,,,,[][1],.35.,1993,(9):540.[2]..,1992,(7):1.[3],,..,1995,26(2):79.[4],,,..,1995,3(2):105.[5],,,..,1997,22(3):203.Studiesonanti2hyperglycemiceffectanditsmechanismofDendrobiumcandidumWUHao2shu1,XUJian2hua1,CHENLi2zuan2,SUNJi2jun2(1.DepartmentofPharmacologyandToxicology,CollageofPharmaceuticalSciences,ZhejiangUniversity,Hangzhou310031,China;2.ZhejiangTianHuangPharmaceuticalCO.LTD.Hangzhou310012,China)[Abstract]Objective:Tostudytheanti2hyperglycemiceffectanditsmechanismofDendrobiumcandidum(DC).Method:Normalmice,adrenaline2inducedhyperglycemicmice,streptozotocin2induceddiabetic(STZ2DM)ratswereused.Themechanismsoftheanti2hyper2glycemicactionwerestudiedwithradio2immunoassay,immunohistochemicalHRP2SPAstain,etc.Result:DCcouldnotobviouslydecreasetheserumglucoseconcentrationsandinsulinlevelsinnormalmice.Itcouldincreaseseruminsulinlevelsanddecreaseserumglucagonsconcentra2tionsinSTZ2DMrats.TheresultsofimmunohistochemicalstaindemonstratedthatthenumberofisletcellswasincreasedandthatofisletcellswasdecreasedinSTZ2DMrats.Itcouldalsodecreasetheserumglucoseconcentrationsandincreaseliverglucogencontentsinadrenaline2inducedhyperglycemicmice.Conclusion:DChasobviousanti2hyperglycemiceffectsinadrenaline2inducedhyperglycemicmiceandSTZ2DMrats.Itsmechanismsarestimulatingthesecretionofinsulinfromcellsandinhibitingthesecretionofglucagonsfromcells,anditcanprob2ablydecreasethedecompositionofliverglucogenandincreasethesynthesisofliverglucogen.[Keywords]Dendrobiumcandidum;streptozotocin;bloodsugar;insulin;glucagon[]36129220042ChinaJournalofChineseMateriaMedicaVol.29,Issue2February,2004