常见载体引物表最新版(印刷版)

VectorPrimer(F)pAAV-MCSF（β-globin）pACTT7pACT25'AD（同GAL4AD）pAS2-15'BD(同GAL4BD)pB42ADpB42ADFpBACPAK8BAC1/PH-Profor/BaculoVforpBG-T(天根）T7pBI12135SpBK-CMVT7/M13FpBluescriptIISK(+/-)/pBluescriptIIKS(+/-)/pBluescriptKS(+/-)/pBluescriptSK(+/-)T3/M13RpBudCE4.1CMV-Profor/T7pBV220PBV220FpCAMBIA1301（1300）P1pCAMBIA2300RV-M/M13RpCANTAB_5EpCANTAB5-S1/pCANTAB5-S3/pCANTAB5-R1pCAT3-enhancerRVP3pcDNA3.0CMV-F/T7pcDNA3.1T7pcDNA4CMV-F/T7pcDNA5CMV-F/T7pcDNA6CMV-F/T7(优先用T7，CMV-F离MCS约200bp)pcDNAIIT7pCE2.1M13FpCEP4pCEP-FpCF-TM13FpCI（neo）17Base/T7（17Base在T7之前）pCI-neoF(暂时不要用)pCMS-EGFPT7pCMV-3Tag-4AT3/PFLAG-CMV-FpCMV5pCMV5F备注：RV-M=M13R-48，M13-47=M13F-47，我们将说法修正的专业一些。如果对本表中的引物有疑问，请跟纪义芳核实。PCMV6/PCMV6-XL4(5,6)/PCMV-ACV1.5pCMV-MCSF（β-globin）pCMV-MYC/HApCMV-FpCMV-SportM13F/T7pCMV-Tag(KAN+)T3pcoldI/pcoldIIIpcoldI-FpCR2.1-TOPOM13RpCR3.1T7pCS2SP6pCS2+(MCSI)SP6pCS2+(MCSII)T3pDBLEUpDBLeu-FpDNR-LIBM13FpDonarM13FpDONR201SeqL-ApDONR221M13FpDriveT7pDsRED1-C1/pDsRED2-C1pDsRED-ex-C1-FpDSRED1-N1CMV-ProforpEasy-bluntM13RpEasy-E1T7pEasy-E2T7pEasy-T1/pEasy-T1SimpleM13R/RV-MpEasy-T3M13F/T7pECFP-C1EGFP-CforpECFP-N1CMV-ProforpEF/myc/ERpEF-FpEGFP（5'MCS）M13R(距第一个酶切位点29bp）pEGFP（3'MCS）pEGFP-C-5’pEGFP-F/C1/C2/C3EGFP-CforpEGFP-N(KAN+)pEGFP-N-5’pENTR/D(-TOPO)(KAN+)M13F/SeqL-ApET-*(His)(KAN+)T7pET22a(b,c)(AMP+)T7pET24(28,30,49)(KAN+)T7pET32a(b,c)(AMP+)S.tagpET-43.1a(b,c)(+)S.tagpET44(AMP+)T7/s.tagpET50(AMP+)T7/s.tagpETDuet-1(MCSI)pBRrevBampETDuet-1(MCSII)DuetUP2pEYFP-C1pEGFP-C-5’/EGFP-CforpEYFP-N1pEGFP-N-5’/CMV-ProforpFastbacPH-profor/BaculoVforpFastBacDual(MCSI)BaculoVfor/PH-ProforppFastBacDual(MCSII)p10-ProforpFastBacHT_A(_B,_C)pFastBac-fw/PH-ProforpFastBac1PH-Profor/BaculoVforpFastBac1-Gus无正向引物pFastBacHT-CAT无正向引物pFLAG-ATS/MACN26forpFLAG-CMV1pFLAG-CMV-F/CMV-30pFLAG-CMV2CMV30pGAD424GAL4AD(5'AD)pGADGHGAL4AD(5'AD)pGADT75'AD/T7pGADT7-RecT7pGAPZa_A(_B,_C)a-FACTOR/GAP-DH-ProforpGBKT7(KAN+)T7/GAL4-BD-CforpGEM-T(Easy)M13F/T7pGEX-2T/pGEX-2TK/pGEX-3XpGEXfw(同PGEX-F)pGEX-4T-1(2，3）pGEXfw(同PGEX-F)pGEX-5X-1(2,3）pGEXfw(同PGEX-F)pGEX-6P-1(2，3）pGEXfw(同PGEX-F)pGEX-KGpGEXfw(同PGEX-F)pGL2GLP1pGL3-Basic(MCSI)RVprimer3pGL3-Basic(MCSII)RVprimer4pGL3-Control(MCSI)RVprimer3pGL3-Control(MCSII)RVprimer4pGL3-Enhancer(MCSI)RVprimer3pGL3-Enhancer(MCSII)RVprimer4pGL3-Promoter(MCSI)RVprimer3pGL3-Promoter(MCSII)RVprimer4pGS21aT7pinpointTMPinpointPrimerpIRES(MCSI)T7pIRES(MCSII)无正向引物pIRES2-EGFPpIRES2-EGFP.P5/CMV-ProforpIRES-hrGFP-2aCMV-Profor/T3pkk223/pkk223-3pKKfw/tac-ProforpLEXAPLEXA-F/LexAforpLNCXPLNCX-FpLNCX2 CMV-ProforpLXSNPLXSN-F/pLXSNfwpMAL-c2E/p2XmalEpMAL-C2xpMAL-C2x-FpMD18-TRV-MpMD19-TM13-47pOTB7M13F/T7pPC86PPC86-FpPCR-ScriptT3pPIC9K(AMP+、ZEO+）5’AOX/a-factorpPICZ_A(_B,_C)5'AOXpPROEXHTAtac-Profor/RV-MpQE30/40/80LpQE30-FpRK5SP6pRSETT7/XpressforpRSFDuet-1(MCSI)DuetUP1pRSFDuet-1(MCSII)DuetUP2pSHUTTLE-CMVPSHUTTLE-CMV-FpSilencer3.1-H1hygroM13-47pSilencer4.1-CMVpSilencer4.1-FpSilencer2.0-U6T7pSK-CMV(KAN+)T7pSP64/65SP6pSP72SP6pSport1SP6/M13FpSTBlue-1T7pSUPERT7pT7Blue(R)T7pTA(天为时代)T7pTA2M13F/T7(优先使用M13F）pT-AdvM13RpTARGETTMpTarget.F(在T7上游)/T7pThioHisA(B,C)TrxForwardpTO-T7T7pTRC99a-cpTRC99C-FpTriEx1.1_NeoT7pTriPLEx2TriplEx-LD-5´pUC18(19)/118(119)/pUC57-TRV-M/M13RpUC20M13FpUCm-TM13F/T7pVAX1T7/CMV-ProforpwpxlEF-LapWSK29T7/M13F/M13-47pXT7T7pYES2T7pSilencer_4.1-CMV_neoPsilencer-4.1-FPACYCDuet-1（MCSⅠ）ACYCDuetUp1PACYCDuet-1（MCSⅡ）DuetUP2PRK5-flagSP6TOPO-PENTR-SDM13FpCMV-Tag-2BT3pCMV-Tag-3BT3PGL4.10RVprimer3PmirGLOpJET1.2/bluntPJET1.2-FpEGFP-N3CMV-ProforPEarlygate20135SpEarlygate10235SpBabe-puropBabe5'PPZP111M13R/RV-MPPZP201M13R/RV-MpBindT7/GAL4BD/CMV-F/SV40/pGEXrvp3xFLAGCMV14CMV30引物名称序列(5'-3')1492RGGTTACCTTGTTACGACTT27FAGAGTTTGATCCTGGCTCA3.0revGAGTTAGCTCACTCATTAGGC35SGACGCACAATCCCACTATCC3'ADAGATGGTGCACGATGCACAG3'AOXGGCAAATGGCATTCTGACAT3'BDTAAGAGTCACTTTAAAATTTGTATAC5'AD（同GAL4AD）TACCACTACAATGGATGATG5'AOXGACTGGTTCCAATTGACAAGC96gIII（同M13-96）CCCTCATAGTTAGCGTAACGa-FACTORTACTATTGCCAGCATTGCTGCBAC1AACCATCTCGCAAATAAATABAC2ACGCACAGAATCTAGCGCTTBaculoVforTGTTTTCGTAACAGTTTTGBaculoVrevAACGCACAGAATCTAGCGCBGHTAGAAGGCACAGTCGAGGCMV-24TTAGGACAAGGCTGGTGGCMV-30ATAACCCCGCCCCGTTGCMV-ProforATGGGCGGTAGGCGTGCMV-RTCGTTGGGCGGTCAGCC-mycrevCTCTTCTGAGATGAGTTTTTGDuetDOWN1GATTATGCGGCCGTGTACAADuetUP1GATCTCGACGCTCTCCCTDuetUP2TTGTACACGGCCGCATAATCEBVrevGTGGTTTGTCCAAACTCATCEF-1aForwardTCAAGCCTCAGACAGTGGTTCEGFP-NrevCGTCGCCGTCCAGCTCEGFP-CforAGCACCCAGTCCGCCCTGAGCF（β-globin）ATTCTGAGTCCAAGCTAGGCGAL4BDTCATCGGAAGAGAGTAGGAP-DH-ProforGTCCCTATTTCAATCAATTGAAGLP1TGTATCTTATGGTACTGTAACTGGLP2CTTTATGTTTTTGGCGTCTTCCAGPDpromoter-FCGGTAGGTATTGATTGTAATTCTGIRES-RCCTCACATTGCCAAAAGACGITS1TCCGTAGGTGAACCTGCGGITS3GCATCGATGAAGAACGCAGCITS4TCCTCCGCTTATTGATATGCITS5GGAAGTAAAAGTCGTAACAAGGLexAforCGTCAGCAGAGCTTCACCATTGM13-47CGCCAGGGTTTTCCCAGTCACGACM13RCAGGAAACAGCTATGACCRV-MAGCGGATAACAATTTCACACAGGAmalEGGTCGTCAGACTGTCGATGAAGCCmU6F2（mouse）TAGTATCCGACGCCGCCATCN26forCATCATAACGGTTCTGGCNOSF-primerCACCTGATCCCAGCTTGCNOSR-primerCGTCCAGCTCCATGTTGCP1CCAGGCTTTACACTTTATGCP2GCGATTAAGTTGGGTAACGCP12584TTTTCAGTATCTACGATP17110TACCACTACAATGGATGp10-ProforCGGACCTTTAATTCAACCCpAcGP67rvTTGGTTCTTGCCGGGTCPA