量子点在生物医学领域的应用

:(NO.20082123)*:1007-4287(2009)06-0847-03王雅丽,张玉成,张桂珍*(,130033),,,,11.1(QuantumDots,QDs),(Nanocrystals,NCs),!∀100nm,(10nm),,[1-3]1.2QDs,:1.2.1QDs(excitationwavelengths),,(emissionwavelengths)[4,5],,1.2.2QDs,[6],,1.2.3QDs,[7],QDs1.2.4QDs[8],,QDs,,,QDs,2QDs,QDsNie[9]QDs,QDs,3QDs,[10,11],,,EDC,,,[12],QDs,,QDs,,,[13,14],,44.1,,,Wu[7],#847#20096136QDs,QDsIgG,Her2,Her2QDs,,,Her2,,Alexa488,QDsSukhanova[15,16]QDsP(Pgp),PgpMCF7r,(multidrugresistance,MDR),MCF7r,Pgp,QDs,,PgpMCF7,,,,PgpPgp,FITCAlexaFluor,,:Her2erbB/HERP2;Mortalin(70)[17,18],4.2,Gao[19]QDs,,PEG,QDs,,PEG(prostatespecificmembraneantigen,PSMA),,QDs,PSMAQDsPSMA,,PSMA,,#QDs,,QDs,,Kim[20],,,,,,,,,,4.3,Jaiswal[21]QDs,QDsHela,QDs12;QDsQDs,Hela,QDs14,12,QDs,4.4,Lidke[22]QDserbB/HER,QDsEGFerbB1,QDsEGFerbB1,erbB2erbB3,,,#848#ChinJLabDiagn,June,2009,Vol13,No.64.5QDs(DNA)Han[23]QDs,(),QDs,QDs,QDs,,610QDs106,,QDs14,3,,5QDs,QDs,,QDs,,,;QDs;QDsQDs,QDs,,:王雅丽(1978-),女,在读博士,主要研究癌基因表达与调控;张桂珍(1955-),女,教授,博士生导师,主要研究微量营养素胰岛细胞保护的分子机制:[1],,,.[J].,2006,25(5):651.[2]SmithAM,NieSM.Chemicalanalysisandcellularimagingwithquantumdots[J].Analyst,2004,129:672.[3]AlivisatosAP.SemiconductorClusters,Nanocrystals,andQuantumDots[J].Science,1996,271(5251):933.[4]ChanWCW,MaxwellDJ,GaoXH,etal.Luminescentquantumdotsformultiplexedbiologicaldetectionandimaging[J].AnalyticalBiotechnology,2002,13:40.[5]MarcelBJ,MarioM,PeterG,etal.SemiconductorNanocrystalsasFluorescentBiologicalLabels[J].Science,1998,281:2013.[6]MedintzI,UyedaHT,GoldmanER,etal.Quantumdotbioconjugatesforimaging,labellingandsensing[J].NatureMaterials,2005,4:435.[7]WuXY,LiuHJ,LiuJQ,etal.ImmunofluorescentlabelingofcancermarkerHer2andothercellulartargetswithsemiconductorquantumdots[J].NatureBiotechnology,2003,21:41.[8]TrueLD,GaoXH.QuantumDotsforMolecularPatology:TheirTimeHasArrived[J].JMolDiagn,2007,9(1):7.[9]ChanWCW,NieSM.QuantumDotBioconjugatesforUltrasensitiveNonisotopicDetection[J].Science,1998,281:2016.[10]SmithAM,RuanG,RhynerMN,etal.EngineeringLuminescentQuantumDotsforInvivomolecularandCellularImaging[J].AnnalsofBiomedicalEngineering,2006,34(1):3.[11]GoldmanER,MedintzIL,MattoussiH.Luminescentquantumdotsinimmunoassays[J].AnalBioanalChem,2006,384:560.[12]LinZB,SuXG,MuY,etal.MethodsforLabelingQuantumDotstoBiomolecules[J].JNanosciNanotech,2003,4(2):1.[13]GoldmanER,BalighianED,MattoussiH,etal.Avidin:ANaturalBridgeforQuantumDotAntibodyConjugates[J].JAMCHEMSOC,2002,124:6378.[14]LiZH,WangKM,TanWH,etal.Immunofluorescentlabelingofcancercellswithquantumdotssynthesizedinaqueoussolution[J].AnalyticalBiochemistry,2006,354:169.[15]SukhanovaA,DevyJ,VenteoL,etal.Biocompatiblefluorescentnanocrystalsforimmunolabelingofmembraneproteinsandcells[J].AnalyticalBiochemistry,2004,324:60.[16]StsiapuraV,SukhanovaA,ArtemyevM,etal.Functionalizednanocrystaltaggedfluorescentpolymerbeads:synthesis,physicochemicalcharacterization,andimmunolabelingapplication[J].AnalBiochem,2004,334(2):257.[17],,.[J].,2006,15(2):214.[18]BruchezmP.TurningalltheLightson:QuantumDotsinCellularAssays[J].CurrOpinChemBiol,2005,9(5):533.[19]GaoXH,CuiYY,LevensonRM,etal.Invivocancertargetingandimagingwithsemiconductorquantumdots[J].NatureBiotechnology,2004,22:969.[20]SungjeeK,YongTL,EdwardGS.NearinfraredfluorescenttypeIIquantumdotsforsentinellymphnodemapping[J].NatureBiotechnology,2004,22:93.[21]JaiswalJK,MattoussiH,MauroJM,etal.Longtermmultiplecolorimagingoflivecellsusingquantumdotbioconjugates[J].NatureBiotechnology,2003,21:47.[22]DianeSL,PeterN,RainerH,QuantumdotligandsprovidenewinsightsintoerbB/HERreceptormediatedsignaltransduction[J].NatureBiotechnology,2004,22:198.[23]HanMY,GaoXH,SuJZ,etal.Quantumdottaggedmicrobeadsformultiplexedopticalcodingofbiomolecules[J].NatureBiotechnology,2001,19:631.(:2008-12-09)#849#20096136